- BNC-all - ecn.purdue.edu

REMINDER -PQC Seminar Friday, Nov. 11th featuring Prof. Alberto Marino
by Anthrop, Heather L 11 Nov '16

11 Nov '16

[cid:image003.jpg@01D238DF.FDEC25E0]

1 0

3D3C Newsletter - October 2016
by Kwok, Tim 11 Nov '16

11 Nov '16

Dear All, Below please find the sixth issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center. The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news) The four sections in the newsletter are: 3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue 3D in focus – this section presents the current work on a specific 3D cell culture model or technique 3D in publications – this section brings a collection of recent publications on 3D cell culture 3D in meetings – this section includes a list of upcoming meetings related to 3D cell culture The newsletter will be available every two months. If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe. Please contact me if you have questions. Yours Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/3e8e050f-67f…] Volume 6, October 2016 [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/10f15e72-d4d…] 3D at Purdue 3D in Focus 3D in Publications 3D in Meetings 3D at Purdue New Thermogels for Culturing Cells in 3D Structures Kinam Park and Bumsoo Han Culturing cells in 3D structures provides many advantages over 2D models. Cells in 3D structures often behave differently from those in 2D cultures. For example, cancer cells organized in 3D spheroids are often more resistant to anticancer drugs than cells in 2D cultures. Several types of commercially available products allow researchers to produce multicellular spheroids. The products are classified into (i) extracellular matrices based on cellular extracts, (ii) natural polymers, and (iii) cell culture plates with non-cell adherent surface or with no surface for the cells to attach. Each method has its own advantages and limitations. Recently, we developed simple polymeric systems that allow cells to form 3D structures. The polymers are named “3DCellMaker” for its ability to culture various types of cells and cell mixtures in 3D (Fig. 1). 3DCellMakers are inverse themosensitive gels (thermogels), i.e., they remain in liquid form at room temperature or lower and become a gel at 37 °C. These thermogels are poloxamers-poly(ester-urethane) copolymers or stearate-modified methyl cellulose. For the culture process, the cells can be placed on top of the formed gels, or they can be mixed with the thermogels at low temperature before incubating at 37 °C. The simplicity, and low cost, of using 3DCellMaker allows culturing of various cell types in 3D for high throughput screening. The synthetic nature of the 3DCellMaker makes it easy to control the gel’s properties. The 3DCellMaker also allows culture of cells with defined additives, under serum-free conditions (Fig. 2). 3DCellMaker thermogels are available for free for researchers at Purdue University. [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/0a41633e-758…] Fig. 1. Co-culture of MCF-7 breast cancer cells and CCD-1068SK fibroblasts in a 3DCellMaker for five days. (Scale bar is 100 •m). [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/f8395e2f-639…] Fig. 2. Culture of T4-2 breast cancer cells after two days in a 3DCellMaker under a serum-free condition (20X objective; courtey picture from the Lelièvre laboratory). ________________________________ 3D in Focus Angiogenesis is the process for new blood vessel formation through sprouting of endothelial cells from existing vessels. This process is regulated by biochemical and mechanical pathways. However, the effects of mechanical stimuli remain poorly appreciated and understood. Recent studies using microfabricated 3D culture systems have highlighted critical roles for flow-induced mechanical stimuli in angiogenesis. In this newsletter we are highlighting the work of Kim and colleagues on the role of mechanical stimuli in angiogenic sprouting using microfluidics. Sudong Kim, Minhwan Chung, Jungho Ahn, Somin Leeb and Noo Li Jeon “Interstitial flow regulates the angiogenic response and phenotype of endothelial cells in a 3D culture model” Lab Chip, 2016, 16, 4189 - 4199 The authors have designed a microfluidic system with six parallel microchannels for the investigation of individual or combined effects of biochemical and mechanical factors during the initiation and outgrowth of angiogenic sprouts within the extracellular matrix. One of the two central channels is loaded with a fibrin matrix in which endothelial cells (ECs) are embedded (channel N); the other central channel contains an acellular fibrin matrix (channel S). The two outermost channels are used for the culture of stromal fibroblasts in the presence of fibrin. Fluidic channels located in-between the central and outermost channels are filled with culture medium with a flow at physiological level 0.1 – 4 μm S-1. The intent is for the soluble proangiogenic factors secreted by the fibroblasts to act on the ECs via the fluidic channels. The initial phase of angiogenesis includes angiogenic sprout, vacuole formation within endothelial cells, and formation of a vascular network. Under static conditions in the device, angiogenic sprout extended from the cytoplasm of ECs (channel N), and these cells initiated vacuole formation that progressively developed into complex vascular networks. The sprouts extended first near the interface of channels N and S, and further into adjacent channel S where the ECs organized multicellular structures characterized by abundant actin-rich filopodia at their edges distal from the N channels thus, morphologically replicating the sprout tip of endothelial cells in vivo. Moreover, the interstitial flow in the S to N direction in the fluidic channels promoted the formation of sprouts with a significantly greater number of filopodia projections compared to those formed under static conditions. In contrast, interstitial flow in the N to S direction completely inhibited the angiogenic sprouting. Unlike angiogenic sprouts, vacuole formation and the development of a vascular structure in channel N were not affected by the direction of the flow. Additional experiments confirmed that the interstitial flow controlled the initiation and sustained the growth of angiogenic sprouting. Reversing the direction of the interstitial flow to S-N abolished the suppressor effect of N-S flow on sprouting. On the other hand, reversing the flow direction from S-N to N-S had no effect on the existing vascular structure, but it induced less filopodia projections and the retraction of the forefront of the vasculature. The model was subsequently examined for its capability to evaluate endothelial responses (i.e., sprout initiation, formation of vacuoles, vascular network formation) to extrinsic proangiogenic factors, such as VEGF, as well as anti-angiogenic compounds such as axitinib. Angiogenesis is fundamental to many physiologic and pathologic processes such as cancer, ischemic diseases, and chronic inflammation. By acting as a proangiogenic stimulant, the interstitial flow in blood vessels can modulate the endothelial responses triggered by the biochemical stimuli such as VEGF. The authors propose that the device can help elucidate the relevant mechanisms and thus, may lead to the development of effective therapeutics for diseases associated with angiogenesis. The model designed here is interesting because of the addition of fibroblasts compared to the usual stand-alone endothelial cells to study angiogenesis within a microfluidics platform. The fibroblasts provide the angiogenic factors, making the model more physiologically relevant. ________________________________ 3D in Publications Recent publications on 3D culture (please click to access the list on our web page https://nanohub.org/groups/3d3cfacility) Review<https://nanohub.org/groups/3d3cfacility/news#reviews> The research articles and reviews are arranged in the following categories: Scaffold free/Scaffold Organ/Tissue/Cell Others Spheroids<https://nanohub.org/groups/3d3cfacility/news#spheroids> Organoid<https://nanohub.org/groups/3d3cfacility/news#organoid> Scaffold<https://nanohub.org/groups/3d3cfacility/news#scaffold> Hydrogel<https://nanohub.org/groups/3d3cfacility/news#hydrogel> Matrix<https://nanohub.org/groups/3d3cfacility/news#matrix> Microfluidics<https://nanohub.org/groups/3d3cfacility/news#microfluidic> Microfabrication<https://nanohub.org/groups/3d3cfacility/news#microfabrication> Bone<https://nanohub.org/groups/3d3cfacility/news#bone> Bone Marrow<https://nanohub.org/groups/3d3cfacility/news#bonemarrow> Breast<https://nanohub.org/groups/3d3cfacility/news#breast> Colon<https://nanohub.org/groups/3d3cfacility/news#colon> Heart<https://nanohub.org/groups/3d3cfacility/news#heart> Liver<https://nanohub.org/groups/3d3cfacility/news#liver> Lung<https://nanohub.org/groups/3d3cfacility/news#lung> Muscle<https://nanohub.org/groups/3d3cfacility/news#muscle> Nerve<https://nanohub.org/groups/3d3cfacility/news#nerve> Prostate<https://nanohub.org/groups/3d3cfacility/news#prostate> Endothelial cells<https://nanohub.org/groups/3d3cfacility/news#endothelialcells> Fibroblast<https://nanohub.org/groups/3d3cfacility/news#fibroblast> Stem Cells<https://nanohub.org/groups/3d3cfacility/news#stemcells> Stromal Cells<https://nanohub.org/groups/3d3cfacility/news#stromalcells> Cancer/Tumor<https://nanohub.org/groups/3d3cfacility/news#cancer> Screening<https://nanohub.org/groups/3d3cfacility/news#screening> 3D bioprinting<https://nanohub.org/groups/3d3cfacility/news#3dbioprinting> Imaging<https://nanohub.org/groups/3d3cfacility/news#imaging> ________________________________ 3D in Meetings 14th Annual High-Content Analysis & 3D Screening Date: 31st October to 2nd November 2016 Location: Cambridge, MA, USA Website: http://www.highcontentanalysis.com/ Contact person: Jaime Hodges Event will deliver the latest advancements in HCA applications and technologies, and the next steps for physiologically relevant complex models, ultra-high resolution and high-throughput imaging, and more advanced image analysis and data management. Organized by: Cambridge Healthtech Institute SPIE BiOS 2017 - Part of SPIE Photonics West 2017 Date: 28th January to 2nd February 2017 Location: San Francisco, CA, USA Website: http://spie.org/SPIE-BiOS-conference Contact person: Customer Service BiOS 2017, part of SPIE Photonics West 2017, is the world’s largest biomedical optics and biophotonics conference. Topics range from biomedical optics, photonic diagnostic and therapeutic tools and systems, nano/biophotonics, and more! Organized by: SPIE - The international society for optics and photonics Deadline for abstracts/proposals: 18th July 2016 3D Cell Culture 2017 Date: 22nd to 23rd February 2017 Location: London, United Kingdom Website: https://go.evvnt.com/69495-0 Contact person: Honey de Gracia Organized by: SMi Group Deadline for abstracts/proposals: 21st February 2017 Keystone Symposia - Engineered Cells and Tissues as Platforms for Discovery and Therapy Date: 9th to12th March 2017 Location: Boston, Massachusetts, United States Website: https://www.keystonesymposia.org/17K1 Contact: Phone: 1 800-253-0685; Email: info(a)keystonesymposia.org<mailto:info@keystonesymposia.org> Organized by: Keystone Symposium

1 0

NSAC Coffee Hour Announcement
by Anthrop, Heather L 11 Nov '16

11 Nov '16

[Inline image 1] NSAC Coffee Hour Speaker: Tejasvi Parupudi Title of the Talk: Implantable fluidic-flushable electrodes for electrophoretically-mediated drug delivery Venue: BNC Attrium Date: 10/28/2016 Time: 3:30pm

1 0

PQC seminar on Nov. 11(Fri): Quantum Enhanced Plasmonic Sensors
by Mikhail Shalaginov 09 Nov '16

09 Nov '16

Friendly reminder that this week there will be a PQC seminar: "Towards Quantum Enhanced Plasmonic Sensors" given by Prof. Marino Time & place: 12-1pm in BRK 2001 Please see the flier attached. Regards, -- Mikhail Y. Shalaginov PhD candidate Research assistant Purdue University Birck Nanotechnology Center 1205 West State Street West Lafayette, IN 47907-2057 tel: +1 765 496 2067; fax: +1 765 496 2018 email: shalaginov(a)purdue.edu https://engineering.purdue.edu/~mshalagi/

1 0

Physics Colloquium Thursday, November 10
by Turner, Jaime J 09 Nov '16

09 Nov '16

[cid:image001.png@01D23A89.623CFBA0]

1 0

2nd floor men's bathroom closed tomorrow until 10:00AM
by Totten, Mary J 09 Nov '16

09 Nov '16

Everyone, Tomorrow, the 2nd floor men's restroom will be closed for a thorough cleaning until 10:00AM. The 1st floor restroom will be open for use. Please let me know if you have any questions. Thank you, Mary Jo Totten Birck Nanotechnology Center Discovery Park/Purdue University 1205 West State Street BRK 1220 West Lafayette, IN 47907-2057 (765) 496-1173 Our greatest weakness lies in giving up. The most certain way to succeed is always to try just one more time. Thomas A. Edison

1 0

Ganesh Hegde Seminar - Tues., Nov. 8th at 10:30am in BRK 1001
by Turner, Jaime J 07 Nov '16

07 Nov '16

[cid:image001.png@01D238F7.2B3755D0]

1 0

PQC Seminar Friday, Nov. 11th featuring Prof. Alberto Marino
by Anthrop, Heather L 07 Nov '16

07 Nov '16

[cid:image003.jpg@01D238DF.FDEC25E0]

1 0

Condensed Matter Seminar, Friday, November 4, 2016, PHYS Room 203, 3:30 PM Today
by Black, Nancy Lee 04 Nov '16

04 Nov '16

Professor Claudio Chamon Department of Physics, Boston University Braiding Light Today Friday, November 4, 2016 3:30 PM, Physics, Room 203 (Refreshments 3:00 PM, Physics, Room 242) Thanks Mike Manfra

1 0

BNC E-news for the week of Nov. 7th
by Anthrop, Heather L 04 Nov '16

04 Nov '16

Characterization & Measurements Methods A user suggested we create a database of available characterization and measurement methods & tools. This is an excellent idea, and should also be applied to other sorts of processing tools. We have two changes coming very soon that may provide this information: 1) our new iLabs scheduling tool is scheduled to be deployed in December and January; and 2) a new Birck website will be launched prior to Thanksgiving. Please look at these two enhancements in our operation, and give us feedback about this suggestion. Another longer-term project is creating a Birck Wiki, but this will be later in 2017. CTSI-Purdue Retreat This message is being sent on behalf of Dr. Thomas Sors, Chief Scientific Liaison, Bindley Bioscience Center, Purdue University The Indiana CTSI-Purdue Retreat is being held at Purdue on Tuesday, December 13 in the Discovery Learning Research Center. We have a very exciting program featuring new investments in the life sciences at Purdue and Notre Dame, with presentations by Donna Fekete, Richard Kuhn and Paul Bohn. We will also highlight cancer prevention research at Purdue, the Indiana University School of Medicine and at IU-Bloomington with speakers Jim Fleet, Roberto Pili and James Klaunig. Afternoon breakout sessions have been organized into highly interactive meetings that are geared to maximize investigator connections to stimulate collaborations. Please see the attached agenda for further details and forward this invitation to other colleagues that may be interested in participating. If you plan to attend, please register by Friday, December 2 at the following link http://j.mp/2d5xGuW. We look forward to seeing you there. Tommy Thomas G. Sors, PhD Chief Scientific Liaison Bindley Bioscience Center Discovery Park, Purdue University Office: BIND 208 Telephone: 765-494-1678<tel:765-494-1678> Purchasing Reminder - You MUST use the attached from when making a credit card purchase (i.e. Amazon). All orders (ariba or credit card) MUST have an explanation of how the purchase benefits the project. Reminder About Lab Glove Policy Users have been observed touching door handles and other 'community' surfaces with gloves on. Please remember our 'one glove' rule that states to never touch door handles or phones in labs with a gloved hand because the surface may become contaminated with some dangerous material that could be on the glove. Rather take your glove off to answer phones and touch door handles with your bare hand to eliminate this possibility. Thanks for your cooperation in making Birck as safe as possible.

1 0