July 2020 - BNC-all - ecn.purdue.edu

REMINDER: BNC and Bindley Joint Virtual Seminar: Dr. Nate Cermak - TODAY, July 31st @ 11:00am via WebEx
by Turner, Jaime J 31 Jul '20

31 Jul '20

BNC and Bindley Joint Virtual Seminar: Dr. Nate Cermak JULY 31, 2020 11:00 AM - 12:00 PM WEBEX<https://purdue.webex.com/purdue/j.php?MTID=md6feecfc0952c8b05271026b0fe633a6> Title: Building new instruments to study biological processes, from single cell growth to whole-organism behavioral coordination Abstract: I will talk about two research projects. In the first, I'll discuss a microfluidic method I developed during my PhD to make precise, high-throughput measurements of single-cell biomass accumulation. This method utilizes an array of suspended microchannel resonators (SMRs), which are silicon microcantilevers with an interior fluidic channel. As cells in suspension flow through the interior fluidic channel, they transiently change the mass of the microcantilever, changing its resonant frequency, which we measure. To measure cell growth, We developed a "serial SMR array", consisting of 10-12 SMRs interspersed along a single long microfluidic channel. Cells are periodically weighed over a span of minutes as they flow through this channel. The change in a cell's mass between when it enters and exits the channel tells us how fast it is growing. Importantly, an entire queue of cells can transit this array nearly simultaneously, yielding growth rate measurements of hundreds of cells per hour. These measurements are significantly more precise than is achievable by microscopy or other existing methods, enabling us to quantify growth rates during a fraction of the cell cycle and see rapid responses to perturbations. In the second part, I'll discuss some of my postdoc work to understand behavioral coordination and its generation by small nervous systems. The hermaphrodite of the nematode C. elegans has only 302 neurons and 143 muscle cells, yet displays a range of motor behaviors including changes in body posture (typically coupled to locomotion), feeding, egg-laying and defecation. Because C. elegans is small and transparent, it is feasible to observe all of its motor behaviors by microscopy, yet long-term behavioral studies have been hampered by an inability to perform long-term tracking and automated behavioral analysis. I'll discuss our development of a new low-cost (scalable) microscopy platform and software suite for analyzing C. elegans behavior. With this platform, we identified a previously unknown role for the neurotransmitter dopamine in regulating egg-laying, that likely had not been previously identified because it is locomotor-state-dependent. Bio: My background is a mixture of biology and hardware and software engineering, and I am especially interested in developing new instruments for biological research. I am currently a neuroscientist/engineer working at Neuralink, a startup developing a high-bandwidth brain machine interface. In my postdocs, I studied neuronal information processing using multiphoton microscopy, as well as neuronal mechanisms of behavioral coordination in C. elegans by building a custom tracking microscope and image processing suite. Prior to that, my PhD work focused on developing microfluidic instruments for ultra-precise measurements of single-cell mass, growth rates, and mechanical properties. WebEx Link: HERE<https://purdue.webex.com/purdue/j.php?MTID=md6feecfc0952c8b05271026b0fe633a6> Meeting number (access code): 120 054 9023 Meeting password: j3G7KvTbp5R Video address: Dial 1200549023(a)purdue.webex.com<mailto:1200549023@purdue.webex.com> You can also dial 173.243.2.68 and enter your meeting number. Audio connection: +1-855-282-6330 US TOLL FREE +1-415-655-0003 US TOLL Show toll-free dialing restrictions<https://www.webex.com/pdf/tollfree_restrictions.pdf> Access code: 120 054 9023 Thank you! Jaime Turner Lead Administrative Assistant to the Director | Birck Nanotechnology Center BRK | 1205 W State Street | West Lafayette, IN 47907 o: 765-494-3509<tel:7654943509> | m: 765-491-3064<tel:7654913064> | jjturner(a)purdue.edu<mailto:jjturner@purdue.edu> [83324AA6]<https://www.purdue.edu/>

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BNC and Bindley Joint Virtual Seminar: Dr. Nate Cermak - July 31st @ 11:00am via WebEx
by Turner, Jaime J 28 Jul '20

28 Jul '20

BNC and Bindley Joint Virtual Seminar: Dr. Nate Cermak JULY 31, 2020 11:00 AM - 12:00 PM WEBEX<https://purdue.webex.com/purdue/j.php?MTID=md6feecfc0952c8b05271026b0fe633a6> Title: Building new instruments to study biological processes, from single cell growth to whole-organism behavioral coordination Abstract: I will talk about two research projects. In the first, I'll discuss a microfluidic method I developed during my PhD to make precise, high-throughput measurements of single-cell biomass accumulation. This method utilizes an array of suspended microchannel resonators (SMRs), which are silicon microcantilevers with an interior fluidic channel. As cells in suspension flow through the interior fluidic channel, they transiently change the mass of the microcantilever, changing its resonant frequency, which we measure. To measure cell growth, We developed a "serial SMR array", consisting of 10-12 SMRs interspersed along a single long microfluidic channel. Cells are periodically weighed over a span of minutes as they flow through this channel. The change in a cell's mass between when it enters and exits the channel tells us how fast it is growing. Importantly, an entire queue of cells can transit this array nearly simultaneously, yielding growth rate measurements of hundreds of cells per hour. These measurements are significantly more precise than is achievable by microscopy or other existing methods, enabling us to quantify growth rates during a fraction of the cell cycle and see rapid responses to perturbations. In the second part, I'll discuss some of my postdoc work to understand behavioral coordination and its generation by small nervous systems. The hermaphrodite of the nematode C. elegans has only 302 neurons and 143 muscle cells, yet displays a range of motor behaviors including changes in body posture (typically coupled to locomotion), feeding, egg-laying and defecation. Because C. elegans is small and transparent, it is feasible to observe all of its motor behaviors by microscopy, yet long-term behavioral studies have been hampered by an inability to perform long-term tracking and automated behavioral analysis. I'll discuss our development of a new low-cost (scalable) microscopy platform and software suite for analyzing C. elegans behavior. With this platform, we identified a previously unknown role for the neurotransmitter dopamine in regulating egg-laying, that likely had not been previously identified because it is locomotor-state-dependent. Bio: My background is a mixture of biology and hardware and software engineering, and I am especially interested in developing new instruments for biological research. I am currently a neuroscientist/engineer working at Neuralink, a startup developing a high-bandwidth brain machine interface. In my postdocs, I studied neuronal information processing using multiphoton microscopy, as well as neuronal mechanisms of behavioral coordination in C. elegans by building a custom tracking microscope and image processing suite. Prior to that, my PhD work focused on developing microfluidic instruments for ultra-precise measurements of single-cell mass, growth rates, and mechanical properties. WebEx Link: HERE<https://purdue.webex.com/purdue/j.php?MTID=md6feecfc0952c8b05271026b0fe633a6> Meeting number (access code): 120 054 9023 Meeting password: j3G7KvTbp5R Video address: Dial 1200549023(a)purdue.webex.com You can also dial 173.243.2.68 and enter your meeting number. Audio connection: +1-855-282-6330 US TOLL FREE +1-415-655-0003 US TOLL Show toll-free dialing restrictions<https://www.webex.com/pdf/tollfree_restrictions.pdf> Access code: 120 054 9023 Thank you! Jaime Turner Lead Administrative Assistant to the Director | Birck Nanotechnology Center BRK | 1205 W State Street | West Lafayette, IN 47907 o: 765-494-3509<tel:7654943509> | m: 765-491-3064<tel:7654913064> | jjturner(a)purdue.edu<mailto:jjturner@purdue.edu> [83324AA6]<https://www.purdue.edu/>

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BNC FY21 Recharge Rates
by Reger, Ronald K 23 Jul '20

23 Jul '20

Dear Birck Research Community, As you know we have been working for several weeks to study and re-do our recharge rates which hadn't been updated for several years. This effort has been led by Justin Wirth, a member of the Birck engineering staff, and he has done an exceptional job in simplifying the approach and in making it much easier to update rates in the future. Many kudos to Justin, the entire Business office management staff, and Purdue Cost Accounting as this approach will likely become a go-to model going forward. Please see attached for Birck's FY21 recharge rates, effective July 1, 2020. Here are some important changes but please read the attached for more information on all of these topics: - Tools are now grouped into "services". The cost for each service is listed in the attached. Individual tools are now grouped in iLab under their service name. - All equipment is now charged hourly, with the exception of metallization systems (evaporators, sputterers, electroplaters, and pulsed laser deposition systems are charged per use) and much of the recharge equipment in the Bio Labs (BASi, Bio Confocal, Gamry, Laser Capture Microdissection System, Vacuum Oven, Zetasizer Nano, KOH Bath System), which are now covered under the daily Bio Lab rate. - Cleanroom optical microscopes (Nikons, Keyence) are now included in the "General Metrology" service. Previously they were included in the cost of cleanroom access. Interlocks will be added in the near future, along with tablet kiosks for easy enabling via the iLab Kiosk interface. - Cap changes: Caps, which apply to internal users only, have been changed. The Monthly Lab Access Cap has been increased from 8 days/month to 15 days/month. The Scifres Equipment Service Cap is now set at a hard cap of $12,000, and is uncapped after a user receives $12,000 (100% the cap level) through the cap. - Lab Access Fees: Entry into any BRK lab/galley (this includes the cleanroom as well) is now charged the Birck Lab Fee ($10/day for internals), and depending on the lab type, lab surcharges are additionally charged. Each type of fee/surcharge is only charged once per day, and it may be possible to be charged the Birck Lab Fee and all four surcharges for one day of access. All labs/galleys have an associated surcharge on top of the Birck Lab Fee. - Cleanroom Surcharge (BRK 2100A): $27/day for internals - Bio Labs Surcharge (BSL1/2 Labs): $6/day for internals - General Labs Surcharge (any other labs/galleys): $2/day for internals - 3D3C Surcharge (BRK 2087): $18/day for internals, charged in addition to the Bio Labs Surcharge. - Due to some minor technical reasons related to how staff time can be distributed to each service, a few of the service prices have changed slightly from when they were previously shared with faculty. The only ones that have changed more than one or two dollars per hour/use are (internal rates): - Advanced Packaging and Assembly: $37/hr now instead of $40/hr - General Roll to Roll: $112/hr vs. $120/hr - Maxwell Roll to Roll: $175/hr vs. $200/hr - Engineering Staff Time: $66/hr vs. 61 /hr - Prices for external users are rounded up to the nearest dollar, and as before, include F&A costs in the listed price. The teamwork among the engineering staff, Birck management, the Business Office, and Cost Accounting was exceptional! Thanks much for your support of this effort. Please let us know if you have any questions. Ron Reger and the Birck Management Team

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FW: PPE Supplies for Laboratories
by Turner, Jaime J 14 Jul '20

14 Jul '20

Forwarded on behalf of Steve Jurss All, PPE supplies are no longer ordered through the EVPRP weekly ordering system. Birck will supply any needed items at no cost. Please see Lisa Strauser lstrause(a)purdue.edu<mailto:lstrause@purdue.edu> for any PPE needs you may have. Stephen Birck Safety Officer

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EM Core Open for Business
by Rosa Diaz 04 Jul '20

04 Jul '20

Dear all, We sent an email to all EM users via iLab a couple of weeks ago asking for you to read the EM SOP, sign it, and send it back to us for approval. The SOP document has specific instructions on how to send this file back to us. If you send the document in the wrong format, this is telling us you havent read the whole document completely. It is really important that you guys read the instruction of the SOP, not only to send the file properly, but also to learn all the protocols we are implementing for your safety and the safety of our staff. I'm attaching the document here as well for those who need it. In the future, please read, or check your SPAM email, for communications from the EM core sent via iLab. Hope you are having a great weekend and happy 4th of July! Rosa *─────────**─────────**─────────**───────**─* *Rosa E. Diaz, PhD* *Electron Microscopy Research Scientist * *Birck Nanotechnology Center* *Purdue University* *1205 W. State Street, West Lafayette, IN 47907-2057 * *Office: BRK-1272 Tel :**765-496-1075* *─────────**─────────**─────────**───────**─*

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New ticketing system for Birck/Scifres equipment support
by Turner, Jaime J 01 Jul '20

01 Jul '20

Sent on behalf of Rich Hosler Today the engineering staff would like to open up the new equipment support ticketing system to the general community. The goal of this system is to reduce downtime and improve communication regarding tool and equipment availability. In general we envision this tool to be the first point of contact for any support requests regarding equipment or services inside BNC. I would ask that if you have any issues or questions regarding equipment in the Scifres Nanofabrication facility or with equipment residing in the BNC, please use the webpage below to submit a ticket. You do not have to create an account to put a ticket in, but it will help you track your tickets better if you do. https://bircknano.freshdesk.com/support/home All iLabs-tracked equipment should be represented in the list by the iLabs name. If you see a piece of equipment missing either put in a ticket for 'other' or email hosler0(a)purdue.edu<mailto:hosler0@purdue.edu> and I'll get the issue resolved. If you're unsure of which equipment to report under you can either select the general core that best represents the system or 'unknown/other'. Under the 'group' tab please select 'research engineering' if this is regarding equipment operation, otherwise there are categories for administration, iLabs billing, and building/installation issues. If you are a tool/process owner and a ticket comes in for your equipment, I'll work to get you set up with an agent account so that you can respond to the ticket from Freshdesk when your first ticket comes in. Any other questions you may have can be reported to hosler0(a)purdue.edu<mailto:hosler0@purdue.edu> and I'll do my best to get you an answer quickly. Thanks for your assistance, Rich Hosler

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