June 2018 - BNC-all - ecn.purdue.edu

Effective immediately: solvent/acid hood protocol enforcement
by Weinstein, Dana 29 Jun '18

29 Jun '18

Dear all, For the safety of our users and staff, as well as for the smooth operation of our cleanroom and labs, we will increase enforcement of solvent/hood protocols effective immediately. Users have all been trained on how to correctly use the hoods, including Personal Protective Equipment (PPE) and labeling their beakers/chemicals, but if you have any questions or concerns, please ask any of the staff. PPE instructions are on each hood. Labels are available at every hood, and from now on, labeling your beakers with name, chemical, and time initiated will be strictly enforced. Please do so when first setting up your glassware before pouring any chemicals. Violations will be elevated to the Disciplinary Committee and may result in restrictions on cleanroom/lab use. Signs have been posted on the hoods to remind users of some of the more critical protocols, including: • All samples and solutions must be labeled with user name, contents and date in the standard label. Unlabeled items will be discarded. • Do not block the perforated plate with your sample or wipes. They will block the air flow to protect you from fumes. • No heads in the hood. (If you need something you can’t reach, get assistance from the staff). • No cell phones in the hood. Do not touch cell phones with solvent or acid gloves. • Do not use the N2 gun to dry samples outside of the hood. There will be a grace period until Monday morning on beakers/samples currently in the hoods. If you have something unlabeled, please go fix that immediately. It will be discarded (and your glassware forfeited) on Monday. Please feel free to address any questions or concerns on this escalation in policy enforcement to me. Thank you, Dana ——————————————— Dana Weinstein Associate Professor, Electrical and Computer Engineering Associate Director of Operations, Birck Nanotechnology Center, Discovery Park Purdue University https://engineering.purdue.edu/hybridmems

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BNC E-news for the week of June 25th
by Turner, Jaime J 25 Jun '18

25 Jun '18

[cid:image001.jpg@01D40C8B.3731B920] Seminars/Workshops Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php Dry Boxes Periodically we receive requests from research groups who need dry box storage in the cleanroom. We have tried to reasonably honor all these requests but now with the addition of many new instruments and pieces of processing equipment the cleanroom is becoming more crowded and space is at a premium. From now on please address any requests for dry-boxes or other items that require cleanroom or chase space to Ron. We will be implementing a simple review procedure as part of our Engineering Excellence initiatives to control use of critical cleanroom space. Thanks for your cooperation. Ron Reger Floor Cleaning Schedule 6/26/2018 - West entrance hallway (Please use main entrance to enter Birck until 10:30AM) 6/27/2018 - BRK 1217 - 1239 (offices closed until 10:30AM) 6/28/2018 - BRK 1072 - 1092 (offices closed until 10:30AM) 7/10/2018 - East Entrance hallway (you will not have access to the color copier until 10:30AM) 7/11/2018 - BRK 1031 - 1049 July 12 BRK 1014 Business Office July 17 BRK 1072 Kitchen/Break Room July 18 BRK 1220 Mailroom ALL CLOSURES ON THIS LIST ARE FROM 5:00am TO 10:30am Please note that you can use the galley entrance to the labs Aspire Four Week Look Ahead Schedule Attached is the Aspire Four Week Look Ahead Schedule. It is the projection of vibration and/or noise issues with the construction outside of Birck.

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BNC E-news for the week of June 18th
by Turner, Jaime J 15 Jun '18

15 Jun '18

[cid:image003.jpg@01D404C4.4DF48A80] Seminars/Workshops Konstantin L. Vodopyanov Seminar "Massively parallel sensing of molecules with mid-infrared frequency combs" June 21st @ 11:00am in BRK 2001 https://www.purdue.edu/discoverypark/birck/events/index.php?view=1570 Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php Dry Boxes Periodically we receive requests from research groups who need dry box storage in the cleanroom. We have tried to reasonably honor all these requests but now with the addition of many new instruments and pieces of processing equipment the cleanroom is becoming more crowded and space is at a premium. From now on please address any requests for dry-boxes or other items that require cleanroom or chase space to Ron. We will be implementing a simple review procedure as part of our Engineering Excellence initiatives to control use of critical cleanroom space. Thanks for your cooperation. Ron Reger Floor Cleaning Schedule 6/19/2018 - The hallway on 1st floor around stairway (stairway & men's restroom closed until 10:30AM) 6/20/2018 -BRK 1099-1201 (both conference rooms closed until 10:30AM) 6/21/2018 - BRK 1261 - 1276 (offices closed until 10:30AM) 6/26/2018 - West entrance hallway (Please use main entrance to enter Birck until 10:30AM) 6/27/2018 - BRK 1217 - 1239 (offices closed until 10:30AM) 6/28/2018 - BRK 1072 - 1092 (offices closed until 10:30AM) 7/10/2018 - East Entrance hallway (you will not have access to the color copier until 10:30AM) 7/11/2018 - BRK 1031 - 1049 July 12 BRK 1014 Business Office July 17 BRK 1072 Kitchen/Break Room July 18 BRK 1220 Mailroom ALL CLOSURES ON THIS LIST ARE FROM 5:00am TO 10:30am Please note that you can use the galley entrance to the labs Aspire Four Week Look Ahead Schedule Attached is the Aspire Four Week Look Ahead Schedule. It is the projection of vibration and/or noise issues with the construction outside of Birck.

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BNC E-news for the week of June 4th
by Turner, Jaime J 01 Jun '18

01 Jun '18

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Seminars/Workshops Purdue's Discovery Park and the Institute for Global Security and Defense Innovation (i-GSDI) will host a Defense Research Information and Strategy Session for Purdue faculty, staff and students. This free event will take place from 10 a.m. to 2:30 p.m. on June 6 at the Burton D. Morgan Center, Room 129. (Light lunch provided.) The purpose of the sessions is to update Purdue faculty, staff, and students on the myriad of opportunities recently released (or expected soon) from the United States Department of Defense (DoD) and related federal agencies. This session will focus on the following DOD priority research areas: * Advanced Computing- Opportunities in quantum, nuero-morphic, and other architectures that are seen as potential enablers of radical new defense and intelligence capabilities * AI+ (AI , Autonomy)-Convergence of novel AI algorithms with enabling technologies in Autonomy/Control, Machine Learning, Efficient Sensing, etc. for a variety of missions * Hypersonics- The ability to understand, develop and field effective and reliable high-speed systems as well as strategies for defense The sessions will include an introduction to the opportunities on where Purdue can contribute and a brief introduction of the top ten technology priorities in the U.S. Defense/Security area. More focused sessions will dive into the top three topic areas with an overview of the landscape in accordance with high level themes/challenges and a discussion on mapping Purdue expertise into the opportunities and strategy for pursuit. Please distribute this information freely to your respective affiliates. Cheers! Tomás Díaz de la Rubia, Ph.D. Chief Scientist and Executive Director, Discovery Park Professor of Materials Science and Engineering, College of Engineering Professor of Strategic Management (by courtesy), Krannert School of Management Purdue University Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php Aspire Four Week Look Ahead Schedule Attached is the Aspire Four Week Look Ahead Schedule. It is the projection of vibration and/or noise issues with the construction outside of Birck.

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3D3C Newsletter - April 2018
by Kwok, Tim 01 Jun '18

01 Jun '18

Dear All, Below please find the fifteen issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center. The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news) The five sections in the newsletter are: 3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue 3D in action - this section highlights recent activity in 3D3C 3D in focus – this section presents the current work on a specific 3D cell culture model or technique 3D in publications – this section brings a collection of recent publications on 3D cell culture 3D in meetings – this section includes a list of upcoming meetings related to 3D cell culture The newsletter will be available every two months. If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe. Please contact me if you have questions. Yours Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/3e8e050f-67f…] Volume 15, April 2018 [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/10f15e72-d4d…] 3D at Purdue 3D in Action 3D in Focus 3D in Publications 3D in Meetings 3D at Purdue Gradient-On-a-Chip to Recreate Heterogenous Microenvironments The Lelièvre and Ziaie laboratories collaborated to design a gradient-on-a-chip (GOC) system in which cells can be exposed to increasing concentrations of chemicals of interest in the same culture chamber. The GOC is made of multiple parts that can be assembled easily with screws and it is reusable (only the paper insert needs to be changed after each culture) (Figure 1). The microfluidic network includes a mixer to create the gradient (Figure 2). The idea behind the design of this cell culture device is that tissue heterogeneity controls cell behavior and in diseases like cancers, promotes progression and treatment resistance. Part of the heterogeneity in cell populations originates from the microenvironment in which chemicals exist in various local concentrations and thus, differently influence cells of a same tissue. Another use of the GOC is to establish concentration thresholds at which a chemical or a drug starts having an effect depending on tissue characteristics. For instance, to validate the use of the system, in their collaborative manuscript (Chittiboyina/Rahimi et al., ACS Biomat Sci & Eng, 2018) the Lelièvre and Ziaie teams demonstrated that a higher tissue stiffness (as seen during breast cancer progression) increased the threshold at which reactive oxygen species (ROS) triggered phenotypic changes, suggesting that cellular response to ROS depends on the microenvironment. Theoretically, the GOC should be usable to also bring cells, like immune cells, in different concentrations to the cultured tissue. [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/709df589-a87…] Figure 1. Components of the Gradient-on-a-chip. (E) From left to right: top acrylic fixture with cell culture aperture, PET membrane (with microapertures) allowing access to the chemical gradient underneath and bringing the necessary stiffness to maintain the paper-based mixer inside the PDMS channels, paper microfluidic insert fitted to the PDMS channels to prevent leakage, PDMS microfluidic platform with laser ablated channels, and bottom acrylic fixture; the inset is a high-magnification image of the laser-machined microapertures on the PET membrane. (F) Final assembled device. Scale bars: (E) 1 cm, (F) 1.5 cm (From Chittiboyina/Rahimi et al., ACS Biomat Sci & Eng, 2018). [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/06d6066b-766…] Figure 2. Gradient formation. Image of gradient formed with red and blue dyes injected in separate inlets one hour before. One inlet on the opposite side is used to remove the fluid. The gradient mixer is visible just below to the inlets, before the rectangular culture chamber. The device is assembled with screws. (From Chittiboyina/Rahimi et al., ACS Biomat Sci & Eng, 2018) Sophie A. Lelièvre, D.V.M., Ph.D., LL.M.P.H. Professor, Basic Medical Sciences Coleader, Drug Delivery & Molecular Sensing, PUCCR Scientific Director, 3D Cell Culture Core (3D3C) Facility Coleader, International Breast Cancer & Nutriton (IBCN) ________________________________ 3D in Action 3D3C at the Experimental Biology meeting 3D3C was invited by Jessica Ellis (Purdue Nutrition Science), to organize a workshop under the tutelage of the American Society for Biochemistry and Molecular Biology during the Experimental Biology 2018 Annual Meeting in San Diego, CA. The 90-minute workshop was scheduled on April 22, 2018. Workshop Title: The Art of the 3D Cell Culture--- from Organoids to Organs-on-a-Chip Abstract submitted for the workshop: When 3D cell culture is properly done the cells behave in a manner that allows their organization and function as in vivo. In the workshop participants will become familiar with the concepts used to place cells in an environment appropriate for their needs and for the study of interest, from simple contexts with one cell type, to complex tissue organization, with multiple cellular compartments. They will participate in interactive observations/analyses of cells in 3D culture and build platforms for organs-on-a-chip from kits. Upon completing the workshop, participants will understand why there can be many cell culture models depending on the scientific queries and technical capabilities, with materials ranging from sophisticated polymers to paper; and they will be better prepared to choose a model convenient for their needs. Outcomes: The workshop was very well-received and the room was practically full, with approximately 150 attendees. The first part of the workshop consisted of an interactive one-hour presentation that stimulated many questions and discussion points, with the appreciation from the audience of the importance to think about tissue organization when working with 3D cell culture. For the 30-minute session with hands-on practices, 3D3C explored a new concept for the design of 3D cell culture that we call OCOrigami (Organ-on-a-Chip Origami), which consists of using paper-based kits to construct organ models. Here we used the examples of the lung and the liver. The attendees were very enthusiastic and actively involved in playing with paper folding and blowing into straws. Who ever thought that cell culture was not fun?! ________________________________ 3D in Focus Cells interact mechanically with their microenvironment. On one hand, externally imposed forces on the matrix can impact cell behavior, on the other hand, the forces generated by cells in the microenvironment may propagate through the matrix and influence the mechanics of such extracellular milieu. However, the impact of cell-generated forces remains to be clearly understood. Notably, direct measurements of local matrix stiffness in three-dimensional (3D) contexts, at a cell level, were awaited. By probing the local micromechanics of cells, the authors of this issue’s highlighted studies were able to observe a remarkably far-reaching stiffening gradient generated in response to cell-generated forces in a variety of biopolymer matrices. Yu Long Han, Pierre Ronceray, Guoqiang Xu, Andrea Malandrino, Roger D. Kamma, Martin Lenze, Chase P. Broederszf, and Ming Guo. Cell contraction induces long-ranged stress stiffening in the extracellular matrix, PNAS www.pnas.org/cgi/doi/10.1073/pnas.1722619115<http://www.pnas.org/cgi/doi/10.1073/pnas.1722619115> 3D3C summary of the article: Cells were cultured at a concentration that maintained cells isolated in extracellular matrix infused with latex beads. The local micromechanics of the matrix was probed using optical tweezers to pull the latex beads away from the cell at a constant speed. For human breast cancer MDA-MD-231 cells in collagen matrix, the matrix became substantially stiffer closer to the cells; a nonlinear stiffness gradient, as high as 50 Pa/μm, was observed. Inhibiting contractility of MDA-MB-231 cells with cytochalasin D (a disruptor of actin cytoskeleton) resulted in a strong attenuation of the cell-induced stiffening. For cells with weak contractility, like non-neoplastic human mammary epithelial MCF-10A cells, the stiffening of the surrounding matrix was found to be negligible. In other matrix systems, MDA-MB-231 cells in Matrigel and human umbilical vein endothelial cells (HUVECs) in fibrin gel, both cell types were capable of generating large, extended stiffness gradients along the contraction direction of cells. The strong stiffness gradient occurring in the vicinity of a cell is proposed to be originated from the active forces that the cell exerts through the matrix. The matrix surrounding a strong contractile cell behaves as a network of ropes. In such case, the total contractile force exerted by the cell is conserved over a distance, and the decay of radial stress simply reflects force spreading over an increasing surface area. This buckling based mechanism for long-range stress transmission is supported by observations with confocal reflection microscopy of a larger amount of highly curved collagen filaments in the vicinity of a contractile cell, compared with the situation in which contraction is inhibited with cytochalasin D. Comments from 3D3C: It has been demonstrated that extracellular matrix stiffness has profound impacts on cellular functions (as illustrated for instance in the gradient-on-a-chip work of the Lelièvre and Ziaie laboratories highlighted in this newsletter). In most studies, matrix stiffness is manipulated through matrix composition or applied external physical force. The cell-generated force on matrix stiffness, however, is rarely discussed, although it illustrates perfectly the general concept of dynamic reciprocity between cells and their extracellular matrix proposed by Mina J. Bissell in the early 80s (Bissell et al, J Theor BIol, 1982). Interestingly, as the highlighted studies confirmed the presence of a nonlinear far-reaching stiffness gradients generated by cells in a 3D matrix, we wonder how results would be changed if the cells were organized as a sheet of interacting cells like in a physiologically relevant epithelium. Surely, the strong cell-cell interactions would influence cell-ECM mutual influence. Another point to consider is how cells like fibroblasts, that strongly mechanically interact with the matrix, would sense epithelial cell-generated forces and mitigate such forces inside the matrix. ________________________________ 3D in Publications Recent publications on 3D culture (please click to access the list on our web page https://nanohub.org/groups/3d3cfacility) Review<https://nanohub.org/groups/3d3cfacility/news#reviews> The research articles and reviews are arranged in the following categories: Scaffold free/Scaffold Organ/Tissue/Cell Others Spheroids<https://nanohub.org/groups/3d3cfacility/news#spheroids> Organoid<https://nanohub.org/groups/3d3cfacility/news#organoid> Scaffold<https://nanohub.org/groups/3d3cfacility/news#scaffold> Hydrogel<https://nanohub.org/groups/3d3cfacility/news#hydrogel> Matrix<https://nanohub.org/groups/3d3cfacility/news#matrix> Microfluidics<https://nanohub.org/groups/3d3cfacility/news#microfluidic> Microfabrication<https://nanohub.org/groups/3d3cfacility/news#microfabrication> Bone<https://nanohub.org/groups/3d3cfacility/news#bone> Bone Marrow<https://nanohub.org/groups/3d3cfacility/news#bonemarrow> Breast<https://nanohub.org/groups/3d3cfacility/news#breast> Colon<https://nanohub.org/groups/3d3cfacility/news#colon> Heart<https://nanohub.org/groups/3d3cfacility/news#heart> Liver<https://nanohub.org/groups/3d3cfacility/news#liver> Lung<https://nanohub.org/groups/3d3cfacility/news#lung> Muscle<https://nanohub.org/groups/3d3cfacility/news#muscle> Nerve<https://nanohub.org/groups/3d3cfacility/news#nerve> Prostate<https://nanohub.org/groups/3d3cfacility/news#prostate> Endothelial cells<https://nanohub.org/groups/3d3cfacility/news#endothelialcells> Fibroblast<https://nanohub.org/groups/3d3cfacility/news#fibroblast> Stem Cells<https://nanohub.org/groups/3d3cfacility/news#stemcells> Stromal Cells<https://nanohub.org/groups/3d3cfacility/news#stromalcells> Cancer/Tumor<https://nanohub.org/groups/3d3cfacility/news#cancer> Screening<https://nanohub.org/groups/3d3cfacility/news#screening> 3D bioprinting<https://nanohub.org/groups/3d3cfacility/news#3dbioprinting> Imaging<https://nanohub.org/groups/3d3cfacility/news#imaging> ________________________________ 3D in Meetings 3rd International Conference on 3D Printing in Medicine Date: 4th to 5th May 2018 Location: Halle, Germany Website: http://3dprint-congress.com/ Contact person:congress@bb-mc.com Organized by: boeld communication GmbH 2nd World Congress and Expo on Nanotechnology and Materials Science Date: 25th to 27th June 2018 Location: Dubai, United Arab Emirates Website: https://biocoreconferences.com/nanotechnology2018/index.php Contact person: Hany Organized by: Biocore 3D Tissue Models: Oncology 2018 Date: 8th to 10th May 2018 Location: Boston, MA, USA Website: http://go.evvnt.com/194508-0?pid=80 Contact person: Doug Fairbrother Organized by: Hanson Wade 8th World Congress of Biomechanics, Dublin, Ireland 2018 Date: 8th to 12th May 2018 Location: Dublin, Ireland Website: http://go.event.com/173142-0 Contact person: WCB 2018 Congress office Organized by: World Congress of Biomechanics, Dublin, Ireland 2018 2nd World Congress and Expo on Nanotechnology and Materials Science Date: 25th to 27th June 2018 Location: Dubai, United Arab Emirates Website: https://biocoreconferences.com/nanotechnology2018/index.php Contact person: Hany Organized by: Biocore Deadline for abstracts/proposals: 25th May 2018 EMBL Conference: Microfluidics 2018: New Technologies and Applications in Biology, Biochemistry and Single-Cell Analysis Date: 15th to 17th July 2018 Location: Heidelberg, Germany Website: http://www.embl.de/training/events/2018/MCF18-01/index.html Contact person: events(a)embl.de<mailto:events@embl.de> Organized by: EMBL CCO Gordon Research Conference — Signal Transduction by Engineered Extracellular Matrices Date: 22nd to 27th July 2018 Location: Andover, NH, USA Website: http://www.grc.org//signal-transduction-by-engineered-extracellular-matrice…<http://www.grc.org/signal-transduction-by-engineered-extracellular-matrices…> Contact person: Sarah C. Heilshorn Organized by: Gordon Research Conference PREDiCT: 3D Models Date: 21st to 23rd August 2018 Location: Boston, MA, USA Website: http://go.evvnt.com/224119-2?pid=80 Contact person: Doug Fairbrother Organized by: Hanson Wade 4th International Conference on Bio-based Polymers and Composites Date: 2nd to 6th September 2018 Location: Balatonfüred, Hungary Website: http://www.bipoco2018.hu Contact person: Dóra Tátraaljai Bio-based polymers and their blends, composites. Natural polymers and their modification. Natural fiber reinforced composites. Other raw materials based on natural resources. Biodegradation and environmental issues EMBO|EMBL Symposium: Organoids: Modelling Organ Development and Disease in 3D Culture Date: 10th to 13th September 2018 Location: Heidelberg, Germany Website: http://www.embo-embl-symposia.org/symposia/2018/EES18-08/index.html Contact person: events(a)embl.de<mailto:events@embl.de> Organized by: EMBL The 4Bio Summit Date: 27th to 28th November 2018 Location: Rotterdam, Netherlands Website: http://www.global-engage.com/event/4bio/?utm_source=4BIOEUMP2018 Contact person: Jane Williams Organized by: Global Engage Gordon Research Conference — Biomaterials and Tissue Engineering Date: 28th July to 2nd August 2019 Location: Castelldefels, Spain Website: http://www.grc.org//biomaterials-and-tissue-engineering-conference/2019/<http://www.grc.org/biomaterials-and-tissue-engineering-conference/2019/> Contact person: Jennifer L. West and Brendan A. Harley Organized by: Gordon Research Conference

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