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BNC E-news for the week of August 28
by Willison, Sheryl L 28 Aug '17

28 Aug '17

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Breaking News The installation of the new JEOL JBX 8100FS Electron Beam Lithography System will start on Tuesday September 19! JEOL will have a 3-person crew at Birck to start bringing the 8100FS and its various components into the cleanroom and start the assembly and testing of the JBX 8100FS in P-Bay. It is expected that installation and acceptance will take 8-10 weeks. Once installation and acceptance is completed, it will be showcased to the world at Purdue and Birck. Here are some of the highlights of the new JBX 8100FS- * 100kV * Two exposure modes- high resolution and high throughput modes * Scanning speed of 125MHz * Three cassettes- 6 inch wafer, four inch wafer and a pieces cassette * Minimum line width -less than or equal to 8nm * Overlay +/- 20nm or less * Stitching +/- 20nm or less We will also have a full service contract for 4 years. We are looking forward to adding this new Electron Beam to Birck's research tool set. Ron Reger Seminars/Workshops Large-scale syntheses of cooperatively assembled materials for sodium-sulfur batteries Bryan D. Vogt, Professor, Department of Polymer Engineering Thursday, September 7, 2017 11:00 a.m. in BRK 2001 http://www.purdue.edu/discoverypark/birck/events/index.php?view=1082 Cell Culture Basics - 2 Days Hands-On Training Course September 7 & 8 Course fee: $250 Deadline for registration is Aug. 18, 2017 Questions Dr. Tim Kwok at kwokt(a)purdue.edu<mailto:kwokt@purdue.edu> or 765-494-6697 Toward Better Lives and Stronger Communities - Bringing the Pieces Together Jim McClelland, Executive Director for Drug Prevention, Treatment, and Enforcement for the State of Indiana Tuesday, September 12, 2017 1:30 - 2:30 p.m. Burton D. Morgan Center, Room 121 Reception at 2:30 -3:30, Mann Hall First Floor Atrium www.purdue.edu/discoverypark/dis<http://www.purdue.edu/discoverypark/dis> See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php

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BNC E-News for the week of August 21
by Willison, Sheryl L 18 Aug '17

18 Aug '17

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Flu Shots This fall Flu shots will be available on Friday, October 27 from 7:00 a.m. to 6:00 p.m. in BRK 1001. Purdue United Way September is "Month of Caring" for Purdue United Way campaign as it starts another great year! Online pledging is available again by visiting: www.uwlafayette.org/purdue/campaign<http://www.uwlafayette.org/purdue/campaign> then clicking on "Purdue Employees Give Now" button. If you have any questions, Sheryl Willison is Senior Chair for the Office of the Executive Vice President for Research and Partnerships. Seminars/Workshops Large-scale syntheses of cooperatively assembled materials for sodium-sulfur batteries Bryan D. Vogt, Professor, Department of Polymer Engineering Thursday, September 7, 2017 11:00 a.m. in BRK 2001 http://www.purdue.edu/discoverypark/birck/events/index.php?view=1082 Cell Culture Basics - 2 Days Hands-On Training Course September 7 & 8 Course fee: $250 Deadline for registration is Aug. 18, 2017 Questions Dr. Tim Kwok at kwokt(a)purdue.edu<mailto:kwokt@purdue.edu> or 765-494-6697 Toward Better Lives and Stronger Communities - Bringing the Pieces Together Jim McClelland, Executive Director for Drug Prevention, Treatment, and Enforcement for the State of Indiana Tuesday, September 12, 2017 1:30 - 2:30 p.m. Burton D. Morgan Center, Room 121 Reception at 2:30 -3:30, Mann Hall First Floor Atrium www.purdue.edu/discoverypark/dis<http://www.purdue.edu/discoverypark/dis> See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php

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Cell Culture Basics Training Workshop - September 7 & 8 2017 (2nd Announcement)
by Kwok, Tim 14 Aug '17

14 Aug '17

[https://secure-web.cisco.com/1ynwiBjJk9OEJk3NVOrALTnc2_eak-BNZhCvf3zNlk6X8U…]

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BNC E-news for the week of August 14
by Willison, Sheryl L 14 Aug '17

14 Aug '17

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] New Equipment Installations On Thursday of this week, the old TRE mask-maker was removed from the cleanroom to make way for new equipment installations. Additionally, the vibration damping plate was successfully installed in P-Bay where the new JEOL electron beam lithography system will be located. Thanks for your patience while this work was completed. A new AJA Ion Mill has been ordered and should arrive here at Birck in the October time frame. It is slated to be installed in Q-Bay. The new 40' roll-to-roll system should arrive in September and E-Bay is being prepared to accommodate this new system. All this new equipment keeps Birck on the leading edge of research capability! Ron Reger Birck Nanotechnology Center Wiki We are pleased to announce the launch of BNCWiki<https://wiki.itap.purdue.edu/display/BNCWiki>, a collaborative knowledge space open to all BNC researchers. Anyone subscribed to BNC-all with a current Purdue Career Account may access, edit, and add pages, upload attachments, and make comments. It is hosted on the wiki software Confluence<https://wiki.itap.purdue.edu/> via ITaP<https://www.itap.purdue.edu/learning/tools/confluence.html>, and offers user-friendly editing, easy upload of documents, and granular page access/editing restrictions. We welcome your inputs, comments, and questions. Please access the Quick Start Guide<https://wiki.itap.purdue.edu/display/BNCWiki/Wiki+Guide#WikiGuide-QuickStar…> for more information about the project and how to contribute. If your questions are not answered there, contact Justin Wirth<mailto:jcwirth@purdue?subject=BNCWiki%20Question> for more information or a personal consultation. Birck Printer Migration Update ECN has updated the instructions for mapping the printers due to the migration. If you were out during the summer, please follow these steps. Instructions to the link: http://eng.purdue.edu/jump/5ffca3 If ECN can help with answering any questions, please feel free to let us know. Seminars/Workshops Toward Better Lives and Stronger Communities - Bringing the Pieces Together Jim McClelland, Executive Director for Drug Prevention, Treatment, and Enforcement for the State of Indiana Tuesday, September 12, 2017 1:30 - 2:30 p.m. Burton D. Morgan Center, Room 121 Reception at 2:30 -3:30, Mann Hall First Floor Atrium www.purdue.edu/discoverypark/dis<http://www.purdue.edu/discoverypark/dis> Cell Culture Basics - 2 Days Hands-On Training Course September 7 & 8 Course fee: $250 Deadline for registration is Aug. 18, 2017 Questions Dr. Tim Kwok at kwokt(a)purdue.edu<mailto:kwokt@purdue.edu> or 765-494-6697 See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php

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Cleanroom Equipment Move
by Reger, Ronald K 10 Aug '17

10 Aug '17

Dear all Birck cleanroom users, Today, Thursday August 10th, the west end of the Birck cleanroom will be temporarily closed to allow equipment moves in & out. We have scheduled a professional heavy equipment moving company to move out the old mask-making Electromask system from the mask shop, and they will also move in the heavy vibration damping plate for the new JEOL e-beam lithography system. This work will commence in the morning and will required the photo bays (M & N-Bays) and P-Bay to be closed during this work. This may take a few hours for both jobs to be complete, during which these west bays will be closed. Other bays in the cleanroom will continue to operate as normal. Thanks for your support and patience. Ron

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Cell Culture Basics Training Workshop - September 7 & 8 2017
by Kwok, Tim 31 Jul '17

31 Jul '17

Dear Fellow Scientists, Due to the high demand, the 3D3C facility will organize another Cell Culture Basics - Training Workshop on September 7 and 8, 2017 to accommodate the needs and this workshop is open for registration. The deadline for registration is on August 18 2017. Please see the pamphlet below for details. If you have questions, feel free to contact me. Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://secure-web.cisco.com/1Vyy6n9gCLiZOKzWeVbVj_uqJoLPxWnIlZHaP3uH3JPpWK…]

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BNC E-news for the week of July 31
by Willison, Sheryl L 31 Jul '17

31 Jul '17

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Performing fiber repair work on Thursday, Aug. 3 and Friday, Aug. 4 from 12:00 - 6:00 am. All Purdue University West Lafayette general internet will be disrupted for up to 30 minutes or longer. Cardinal Contracting will be removing the TRE from the clean room to the sub fab on August 10. Because of the size and weight of the TRE, we would like to close the optical litho bays M, N and P during the removal. Tools affected include MJB3 and MA6 aligners, Suss Bonder, Laminator tool, microscopes, hot plates, Nanonex, VB6, Bruker GT, Nanoman AFM, and Hermetic sealer. Please update our users as to this partial clean room shutdown of Bays M, N and P on Thursday, August 10. Cardinal has not given us a starting time but morning hours are likely. Could you place a notice in both this Friday and next Friday? Thanks, Mike NSAC (Nanotechnology Students Advisory Council) is organizing an Undergraduate Research Symposium on Friday, August 4, 2017 from 2 to 4 p.m. in the Birck Atrium. This is a unique opportunity for undergraduates to present their research in front of an audience comprised of graduate students, staff, and faculty and get valuable feedback. Plaques will be awarded to the top three posters as well as certificates to all participants. Please encourage any undergraduates in your respective groups to consider registering using the following link: https://goo.gl/forms/vKZQ1U2Itg8dZyic2. The deadline for registration is Friday, July 28, 2017. Thank you! Sincerely, Deesha Shah Birck Nanotechnology Center Wiki We are pleased to announce the launch of BNCWiki<https://wiki.itap.purdue.edu/display/BNCWiki>, a collaborative knowledge space open to all BNC researchers. Anyone subscribed to BNC-all with a current Purdue Career Account may access, edit, and add pages, upload attachments, and make comments. It is hosted on the wiki software Confluence<https://wiki.itap.purdue.edu/> via ITaP<https://www.itap.purdue.edu/learning/tools/confluence.html>, and offers user-friendly editing, easy upload of documents, and granular page access/editing restrictions. We welcome your inputs, comments, and questions. Please access the Quick Start Guide<https://wiki.itap.purdue.edu/display/BNCWiki/Wiki+Guide#WikiGuide-QuickStar…> for more information about the project and how to contribute. If your questions are not answered there, contact Justin Wirth<mailto:jcwirth@purdue?subject=BNCWiki%20Question> for more information or a personal consultation. Seminars/Workshops COMSOL Multiphysics Tuesday, August 1, 2017 2:00-3:00 p.m. in Mann Hall 203 To register, please visit: http://secure-web.cisco.com/1wnw3BpXKC92u6Zs7NjyuyBlv8WVrpk9bxKCYbsGe0JLPWw… Safe and Secure Systems and Software Symposium (S5) Air Force Research Laboratory (AFRL) August 1-3, 2017 http://www.mys5.org/index.php Park AFM Workshop Park Systems and Seitz Materials Research Laboratory Thursday, August 3, 2017 10:00 AM - 3:00 p.m. at University of Illinois at Urbana=-Champaign Registration link: http://www.parkafm.com/index.php/events/workshops/the-latest-advances-in-af… NSAC Undergraduate Research Symposium Summer 2017 Friday, August 4, 2017 2:00 - 4:00 p.m. Birck Atrium Register https://goo.gl/forms/vKZQ1U2Itg8dZyic2 by Friday, July 28. Toward Better Lives and Stronger Communities - Bringing the Pieces Together Jim McClelland, Executive Director for Drug Prevention, Treatment, and Enforcement for the State of Indiana Tuesday, September 12, 2017 1:30 - 2:30 p.m. Burton D. Morgan Center, Room 121 Reception at 2:30 -3:30, Mann Hall First Floor Atrium www.purdue.edu/discoverypark/dis<http://www.purdue.edu/discoverypark/dis> See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php

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UIUC AFM Workshop
by Reger, Ronald K 28 Jul '17

28 Jul '17

Dear Birck Research Community, Please be aware of an AFM workshop being given Aug. 3 at University of Illinois at Urbana-Champaign, per the attached flyer. FYI. Ron

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3D3C Newsletter - June 2017
by Kwok, Tim 25 Jul '17

25 Jul '17

Dear All, Below please find the tenth issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center. The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news<https://secure-web.cisco.com/1r5XHGGlvadtnYpinflhL_B8eQu58XITk_kTkOoioE6JHZ…>). The four sections in the newsletter are: 3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue 3D in focus – this section presents the current work on a specific 3D cell culture model or technique 3D in publications – this section brings a collection of recent publications on 3D cell culture 3D in meetings – this section includes a list of upcoming meetings related to 3D cell culture The newsletter will be available every two months. If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe. Please contact me if you have questions. Yours Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://secure-web.cisco.com/1MwRbI6gBYnT7ITCVBm9es_3lA0amOhN8Q5PWfIgyDi-P7…] Volume 10, June 2017 [https://secure-web.cisco.com/1Qke7ApeA43xxAbCTHWx2P-MbyhKYh2RazsGPDNa8S73uy…] 3D at Purdue 3D in Focus 3D in Publications 3D in Meetings 3D at Purdue Modeling Blood-Brain Barrier Permeability in an In Vitro Model System The blood-brain barrier (BBB) is the tightest and most effective barrier in the body, and protects the brain from circulating ions, macromolecules or toxins. The BBB is composed of endothelial cells with distinct tight junctions, a specialized basement membrane, pericytes and astrocyte endfeet. Interaction of endothelial cells with other components of the BBB, especially pericytes is paramount in maintaining BBB integrity.1 Models of the BBB are commonly used to investigate permeability characteristics of astrocytes, pericytes, and endothelial cells.2–5 In vitro models are commonly composed of brain derived cells of murine, porcine, bovine, or human origin. Permeability of in vitro models of the BBB is typically measured using transendothelial electrical resistance (TEER) or diffusion of a fluorescent marker; both modalities have a direct correlation to paracellular permeability. In the Comparative Blood-Brain Barrier Laboratory, we utilize an in vitro model of the BBB using a transwell system composed of mouse derived pericytes6, endothelial cells, and astrocytes (Figure 1). In this system, 20,000 astrocytes are placed on the base of a 24-well plate, 20,000 mouse brain derived pericytes on the base of the transwell insert (Corning), and 20,000 mouse brain derived endothelial cells on the apical surface of the transwell insert (Figure 2a). This BBB model was validated by measuring permeability for seven days in brain derived pericytes and endothelial cells with TEER. Within three days, many cells showed evidence of apoptosis. Our laboratory is most interested in alterations of the blood-brain barrier in brain metastases of lung cancer and the formation of the blood-tumor barrier (BTB).7 An in vitro model of the BTB has been established by co-culturing tumor cells, of any origin, with brain-derived astrocytes in the base of a 24-well plate (Figure 2b). In our models of the BBB and BTB, TEER and diffusion of fluorescence dye are the primary modalities used to evaluate permeability. To measure TEER, two linear electric probes attached to an EVOM2 Volt-Ohm Meter (World Precision Instruments) are placed in the upper compartment of the transwell and in the basal compartment of the well. The electric probes are stabilized and a numerical reading is displayed on the Volt-ohm meter representing TEER. In vivo, TEER of a normal rat brain is approximately 1000 ohms/cm2. TEER measurements in the in vitro BBB model vary widely, but are typically measured between 100-3000 ohms/cm2 depending on culture conditions, cell seeding density, and cell origin. Analysis of permeability via diffusion of fluorescence dye, is determined by calculating the diffusion of a fluorescent dye from the apical compartment of a transwell to the basal compartment. The relative fluorescent units (RFU) of the fluorescent dye of the basal compartment is calculated using a spectrophotometer. A “gold standard” does not exist for an in vitro model of the BBB; however, the parallel analysis of TEER and diffusion of fluorescent markers provide the best analysis of permeability of an in vitro model of the BBB and BTB using a transwell system. The use of the in vitro models of the BBB and BTB provide a primary system to evaluate permeability and efficacy of novel therapeutic compounds that may have an effect on metastatic or primary brain tumors in vivo. [https://secure-web.cisco.com/1bdWvv_p58JMRUbip2wuN4L86Gv8laMm9a6pEHcMxDFq4D…]<https://secure-web.cisco.com/16wD976YCX9DzuJ1uKmHLPXxE_kAdeQ6u9UPXCCx35w5pZ…> Figure 1: Mouse brain derived endothelial cells (CD31), astrocytes (GFAP) and pericytes (Desmin and CD13). These cells were used to establish an in vitro model of the BBB. [https://secure-web.cisco.com/1oixdt7GlyyXhDhNIwPwfQ1TVQs3qvvumIkJC-nTmF_O80…]<https://secure-web.cisco.com/1I81J5_iMMJGdl_-GpaCgMB626Bty2xmPOEFsxJCfeidFN…> Figure 2A. An in vitro model of the BBB. Astrocytes are placed on the base of the well, endothelial cells on the apical surface of the transwell insert, and pericytes on the basal surface of the transwell insert. [https://secure-web.cisco.com/1xqUXhJZwaSf91EKXtQNkeL7lk8u-ORIVugWFeQFmUwHxH…] Figure 2B. An in vitro model of the blood-tumor barrier. Cells are arranged as described in 2A; however tumor cells of any origin are grown in co-culture with astrocytes in the base of the well. Tiffany Lyle, DVM, PhD, DACVP Assistant Professor Comparative Blood-Brain Barrier Laboratory Department of Comparative Pathobiology College of Veterinary Medicine ________________________________ 3D in Focus Three-dimensional (3D) printing technology shows promise for creating complex composite tissue constructs through precise placement of cell-laden hydrogels in a layer-by-layer fashion. The technology has numerous applications in medicine, e.g. regenerative medicine for the need of tissues and organs suitable for transplantation. 3D printing provides unprecedented control over scaffold architecture, including the geometry of the culture that may influence cell and tissue behavior. The study in the highlighted article examined how the geometry of the culture in scaffold may affect the growth and maturation of ovarian follicles as well as the development of a bioprosthetic ovary to restore ovarian function. The work was done in mice. Description of the article of interest: Laronda MM, Rutz AL, Xiao S, Whelan KA, Duncan FE, Roth EW, Woodruff TK, Shah RN4. A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice. Nat Commun. 2017 May 16; 8:15261. Scaffolds with different pore geometry were generated by laying a layer of printed gelatin over the layer underneath at the angle of 300, 600 or 900. In 3D confocal image, 300 and 600 scaffolds were observed to have extended corners and another strut underlying the pore, while 900 scaffolds possessed shallow corners without any underlying struts (Figure 1). In vitro, the ovarian follicles thrived best in 300 and 600 scaffolds as compared to the 900 scaffold. Moreover, follicle survival significantly increased with the number of contacts between each follicle and scaffold. When seeded into 300 and 600 scaffolds, each follicle was most likely to have two or three contact points with the scaffold whereas it had mostly one contact point in 900 scaffolds. The 300 and 600 scaffolds provided corners that created contacts with follicles on multiple sides while 900 scaffolds had an open porosity that limited follicle–scaffold interaction. Adhesion of cells, located at the periphery of the follicle, to the scaffold was evidenced by the presence of actin filaments along the surface of the elongated cell bodies and by cell–matrix focal adhesions visualized by staining for the cytoskeletal protein, vinculin. Steroidogenesis is one of major processes occurring within the ovarian follicles that converts cholesterol into steroid hormones, such as estradiol and progesterone. Follicles cultured in the 300 and 600 scaffolds, but not the 900 scaffolds secreted an increased amount of estradiol guiding the follicles toward terminal differentiation and ovulation in vitro, as indicated by the release of mature eggs with extruded polar bodies. The results indicate that the 300 and 600 scaffold architectures support hormone production, oocyte maturation and ovulation. The 300 scaffold were implanted within the ovarian bursa of adult ovariectomized mice (both ovaries were surgically removed). The follicle-seeded scaffolds became highly vascularized and ovarian function was fully restored. Moreover, pups were born through natural mating and thrived through maternal lactation. These observations support the possibility for the bioprosthetic ovary to restore organ function in mice. Comments from 3D3C: 3D printing is rapidly evolving to become a technology with numerous medical applications. There are, however, gaps in information regarding the impact of scaffold architecture on tissue geometry that might, in turn, influence the physiology and function of cells and tissues in contact with the scaffold. The present study is one of a few reports published so far on this issue and it confirms the critical role of tissue geometry influenced by the printed scaffolds for cell and tissue function. Indeed, based on their observations, the authors proposed that the follicles cultured in vitro required multiple contacts with the strut of the scaffolds to maintain a spherical shape, hence enabling cell survival. The exact shape of the follicles within the three scaffolds, however, was not illustrated with actual measurements. These findings will be useful for the future design of effective scaffolds and the improved medical use of 3D printing. [https://secure-web.cisco.com/1PbPjXij7ml99Uwdc-1hoIbf-_Z_i3VLE_R1v-8vITjbM1…]<https://secure-web.cisco.com/1St8q2mrsMcQVlKXR0_Dos0pv2dBFCXqo9wAc9GEkT9qyj…> Figure 1. Sketch for stacks of 300, 600 and 900 angle scaffolds. ________________________________ 3D in Publications Recent publications on 3D culture (please click to access the list on our web page https://nanohub.org/groups/3d3cfacility<https://secure-web.cisco.com/1ZAUfrdGvE6H9WDQpzNNDYHJLm6u9oRpjodPe_ttyM1ptU…>): Review<https://secure-web.cisco.com/1lIQeYoxtKm048VC720h3JZEf6rMZR53-S97q0VNLjLxlm…> The research articles and reviews are arranged in the following categories: Scaffold free/Scaffold Organ/Tissue/Cell Others Spheroids<https://secure-web.cisco.com/186R626zbIfKQ_NUBulpROm6meSy19t7GRXiFYFSL9CAwK…> Organoid<https://secure-web.cisco.com/10I9VdwPSVeY4kkX0wjdqvU_wjXq7cYkM19KaaVlhtAQ7f…> Scaffold<https://secure-web.cisco.com/14xUpM28yC2yDMcyfzJl6YYAZTvDp4jE1FzlFN0R52GB83…> Hydrogel<https://secure-web.cisco.com/1p2ayvGrnl3q06Xv9TQH3F3GT33llTh31YSStynjl_SYKX…> Matrix<https://secure-web.cisco.com/1CvVdQMXzBdO_a1eM3QhDoFQMBESV6GN1CvjD17rkyhZQG…> Microfluidics<https://secure-web.cisco.com/1GsVGftRV3u0AO05kgwOsu5AI-z-VP9BD1S0QrpJZBBW0p…> Microfabrication<https://secure-web.cisco.com/1C_93n_81TFwRoCkIhkoY2RgCQKmkYu-746QfQimT5mpGA…> Bone<https://secure-web.cisco.com/10Kc2BhQv9KV9M3g9jkG4SY_Juu_YQnXzzvORf4ovrbzEl…> Bone Marrow<https://secure-web.cisco.com/1iKq4hPO-FKSJIasGrxbeJ7SEEsuFlm6GGC_Gzl22kM41E…> Breast<https://secure-web.cisco.com/1czedTWazaL-YPBXEIB_uE1D4XO8vN_ojA8gXbGO_n6aXj…> Colon<https://secure-web.cisco.com/1nCgXvZaNAtSViqZiLu63h_VCzvYivU1BPyNwRDuTIiA3R…> Heart<https://secure-web.cisco.com/1Juf7THYlbHESn1nOhn7mtMwmPiEDf7nkZvEFVf6nvzGkr…> Liver<https://secure-web.cisco.com/1oFrdEeFW0pZyxPPE4lAZgilWOjirz5VUmzE4ynNB5NO3k…> Lung<https://secure-web.cisco.com/1BbXt-qGnTJCwcDahJ4px0fYWNSYA48_kRoFx6Iin-Vehe…> Muscle<https://secure-web.cisco.com/17oouE5otcWbab2T9D31uCG98zVH9inVqShGp5l1jjZX6d…> Nerve<https://secure-web.cisco.com/1DwJ16ACYuHiSeAp3g5jShE5YN-KAsmZRfkBUxrB6_uuYt…> Prostate<https://secure-web.cisco.com/12hnirLnNwziYpHWi9EmWaR5CIKlrKHmZP_wRpIlsRRRG6…> Endothelial cells<https://secure-web.cisco.com/1rEszn2fFIM0qNtXFAztxEWNGqhzSU1SreSbHH7QwaP7yN…> Fibroblast<https://secure-web.cisco.com/1DiOXiMBch-r35rxVQi7n5Ar4W4O-K8po0R9zN8RbiF8yH…> Stem Cells<https://secure-web.cisco.com/1Yckab8yErrSqOQ-z3nb4xj9bUFJlG_FmJiFchEyoplVHq…> Stromal Cells<https://secure-web.cisco.com/1i7rgsKHK_-89j1NLqdpfW85RM8kGXEKfyEKSCmZAncFSE…> Cancer/Tumor<https://secure-web.cisco.com/1zHu47JptFW8he_mjZELln4uTJMFq40OBAhFjDTcP4OlKk…> Screening<https://secure-web.cisco.com/1tfMlIR05YPVZAgSTzLWA_lA5d9saPF5rX8JZKXMxW_oct…> 3D bioprinting<https://secure-web.cisco.com/1QDBXwIfmywAi25w61d-HhNJ6-kVnIqAC4RsL_uwh9Nxn2…> Imaging<https://secure-web.cisco.com/1N8udTdmv79dnv_I7g43fZtt0RxdfslLBCWGZIEENBIPdc…> ________________________________ 3D in Meetings Third International Conference on Biomedical Engineering and Science (BIENS 2017) Date: 3rd to 4th July 2017 Location: Geneva, Switzerland Organized by: Third International Conference on Biomedical Engineering and Science (BIENS 2017) Deadline for Submission: February 27, 2017 Microfluidics Congress: USA Date: 25th to 26th July 2017 Location: Philadelphia, USA Website: http://www.global-engage.com/event/microfluidics-congress-usa/<http://secure-web.cisco.com/1CAfq-v0FnK3fgTI4ptcoB5ONqJkYXttj-ZDBonuR256AW4…> Contact person: Jane Williams Organized by: Global Engage At the intersection of engineering, physics, chemistry, nanotechnology, and biotechnology, microfluidics is revolutionizing the way patients are diagnosed, monitored and treated, and is unlocking the potential for reduced reagent consumption and cost 3D Tissue Models 2017 Date: 28th to 30th August 2017 Location: Boston, Massachusette, USA Website: http://go.evvnt.com/120739-0<http://secure-web.cisco.com/1p0H-7r4h-W59jIABzlKuddBCUHVzMRt6L106bV9cdk9SHJ…> Contact person: Customer Services Organized by: Hanson Wade International Conference On Nanomedicine And Nanobiotechnology 2017 Date: 25th to 27th September 2017 Location: Barcelona, Spain Website: http://premc.org/conferences/iconan-nanomedicine-nanobiotechnology/<http://secure-web.cisco.com/13XQJWV_H1URqIvRIJ48W76ctkz06xbWJ-6AcEjBiUsgTms…> Contact person: Samuel HADDAD Organized by: PremC Deadline for abstracts/proposals: 30th June 2017 Bioprinting and 3D Printing in the Life Sciences Date: 17th to 18th October 2017 Location: Cambridge, United Kingdom Website: http://go.evvnt.com/113717-0<http://secure-web.cisco.com/1PcFNrNREEeYOIsKlg6EePnf3dAdqgPCyAUBWdRbrmvBf1W…> or https://selectbiosciences.com/conferences/index.aspx?conf=B3DPLS2017<https://secure-web.cisco.com/1naH4jo0sNipZ5RiAqT4fvi96hzjRp0kOwZzjhb61qzcGh…> Contact person: Sara Spencer Organized by: SelectBIO International Translational and Regenerative Medicine Conference Date: 1st to 3rd November 2017 Location: Barcelona, Spain Website: http://itmc.madridge.com/<http://secure-web.cisco.com/1-Wck8O9nwCa1mHXWnd8kzDCsDNTbVu_dgDYJvr6tDvi6ha…> Contact person: Samatha R High-Content Analysis & 3D Screening Date: 6th to 8th November 2017 Location: Cambridge, Massachusetts, USA Website: HighContentAnalysis.com <http://secure-web.cisco.com/17VSEDtUc_6STLwWlttkpqOChknYnyvr8spVikCgoLNBGRa…> Contact person: Jaime Parlee Organized by Cambridge Innovation Institute World Preclinical Congress Europe 2017 Date: 15th to 17th November 2017 Location: Lisbon, Portugal Website: http://worldpreclinicaleurope.com<http://secure-web.cisco.com/1sLUWrMpSft05q9DbysZTIiUwTPd1ibWQ_elz7PF0FrI_CE…> Contact person: Bethany Gray Organized by: Cambridge Healthtech Institute International Biotechnology and pharmaceutical Industry Forum Date: 11th to 13th December 2017 Location: New Delhi, India Website: http://biopharma-forum.com/#1<http://secure-web.cisco.com/1osEf8Yck8imqJyZL2zdWnhsJXL7BRFzs9gDuRRYRvVT5xy…> Contact person: sangavi Organized by: Clyto access Deadline for abstracts/proposals: 25th April 2017 ITMC-2017-Aims to discover advances in health practice & in relation to health disparities, as well as a breadth of other topics. Need for translational medicine, Challenges in translational medicine, Opportunities in translational medicine. 3d Cell Culture Date: 21st to 22nd February 2018 Location: London, United Kingdom Website: https://www.smi-online.co.uk/pharmaceuticals/uk/3D-Cell-Culture<https://secure-web.cisco.com/1fr8SMRdRXf2Ye02YrmFtMAos2-vQ3jYY7NPVh1GvUijxY…> Contact person: Customer Services Organized by: SMi group

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BNC E-News for the week of July 24
by Willison, Sheryl L 21 Jul '17

21 Jul '17

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Dear Birck Research Community, The business office and engineering organizations have just completed an analysis of current recharge rates. A few rates were found to be incorrect due to recent service contract price changes, usage rate adjustments, etc. A revised rate sheet is being completed and will be put up on the Birck website by the end of July. We have also added a set of equipment that recently had rates approved. You will find those listed on the new rate sheet as well. In addition, as we do every few years, we are in the process of a full Scifres Rate Review. Once we have that completed, we will notify you of any changes. BNC Management [cid:image006.png@01D3022D.EF003B30] The electrical shutdown will be on Saturday, July 29, 2017, from 7:00 am-11:00 am. Birck's parking lot will be affected, weather permitting. West side gravel parking lot is available for parking. Micron Sweps Our custodial staff professionally cleans the cleanroom surfaces with special 'mops' called Micron Sweps. These expensive & reusable mops are used in conjunction with our UltraPure Water to wipe down all surfaces in the cleanroom on a continual basis. We pay a service to clean the Sweps for us, and the laundering is done in a cleanroom environment at the contracted laundry. Recently someone retrieved a Swep and used it to wipe up something in the cleanroom. Please do not use these Sweps! They are designed only to be used with UPW for cleaning cleanroom floors and walls. Other uses will ruin them at a significant cost to Birck. Thanks for your understanding and cooperation. Birck Management Where can you wear shorts in Birck? There has been some confusion as to where shorts are allowed. Long pants or long dresses and closed toed shoes are required to be worn where the potential exists for chemical use. This would include all general labs, bio-cleanroom, J-bay and SEM prep lab. Shorts are allowed in the cleanroom under your cleanroom garment and in the SEM microscope labs. This is for safety concerns and if you have a question, it will always be best to error on the side of safety and wear what provides the most protection. Stephen Birck Safety Officer NSAC (Nanotechnology Students Advisory Council) is organizing an Undergraduate Research Symposium on Friday, August 4, 2017 from 2 to 4 p.m. in the Birck Atrium. This is a unique opportunity for undergraduates to present their research in front of an audience comprised of graduate students, staff, and faculty and get valuable feedback. Plaques will be awarded to the top three posters as well as certificates to all participants. Please encourage any undergraduates in your respective groups to consider registering using the following link: https://goo.gl/forms/vKZQ1U2Itg8dZyic2. The deadline for registration is Friday, July 28, 2017. Thank you! Sincerely, Deesha Shah Birck Kitchen Friday, July 28 around 2 p.m., the refrigerator and freezer will be cleaned out. Anything that is outdated or looks bad with no label will be thrown away. Any questions/concerns see Nancy Birck Nanotechnology Center Wiki We are pleased to announce the launch of BNCWiki<https://wiki.itap.purdue.edu/display/BNCWiki>, a collaborative knowledge space open to all BNC researchers. Anyone subscribed to BNC-all with a current Purdue Career Account may access, edit, and add pages, upload attachments, and make comments. It is hosted on the wiki software Confluence<https://wiki.itap.purdue.edu/> via ITaP<https://www.itap.purdue.edu/learning/tools/confluence.html>, and offers user friendly editing, easy upload of documents, and granular page access/editing restrictions. We welcome your inputs and comments. What is BNCWiki? The Birck Nanotechnology Center Wiki is an effort to document and centralize the experimental expertise currently spread around the facility in individuals and research groups. BNC researchers are encouraged to contribute their processes, experiences, issues, and knowledge in order to increase the speed, quality, and breadth of research that can take place at Birck. BNCWiki currently contains equipment pages for both fabrication and characterization equipment, with details on capabilities, technological overviews, SOPs, user manuals, processes, and references. Info pages are available for iLab, general issues, standard and contributed processes, and MSDS sheets. A wiki is, by its nature, a work in progress. The success of the project will depend on contributions from students, faculty, and staff. Research groups may host their own pages for more specialized info, access to which can be restricted to the group if desired. Please contact Justin Wirth<mailto:jcwirth@purdue.edu?subject=BNCWiki%20Question> for more info. How can I access BNCWiki? Access the main page here: https://wiki.itap.purdue.edu/display/BNCWiki. Login with your Purdue Career Account login and password. The site is accessible through both desktop and mobile devices. If pages show formatting issues on mobile, either the "View Desktop Version" option in Confluence or the "Request Desktop Site" setting in the mobile browser may improve display. How do I edit a page? To enter edit mode when viewing a page, press either the "Edit" button in the top right corner of the page, or press the "e" button on your keyboard. [cid:image007.jpg@01D3022D.DE3BC800] This will launch the Confluence Editor<https://confluence.atlassian.com/conf61/the-editor-877187526.html>. Page editing is similar to a document editor such as Microsoft Word, with formatting options available on the menu bar at the top of the page. After changes are made, the "Preview" button in the bottom right corner will allow you to view the edit before publishing. Once satisfied with the changes, press the "Update" button in the bottom right corner to publish the changes. How do I add a document? Confluence supports native viewing of PDFs and images, as well as Word, Powerpoint, and Excel files. Once in the Editor, add a file by clicking the "Insert files and images" button, or using the shortcut Ctrl+M. [cid:image008.jpg@01D3022D.DE3BC800] Use the "Upload files" button to attach one or multiple files to the page. Then press the "Insert" button to add them to the page. How do I make a comment? A comment section is located at the bottom of each page, with formatting similar to the page editor. Documents and pictures may be embedded if desired. A dedicated page is available for general discussion of Comments, Suggestions, and Issues<https://wiki.itap.purdue.edu/display/BNCWiki/Comments%2C+Suggestions%2C+and…>, and another for Process Help and Troubleshooting<https://wiki.itap.purdue.edu/display/BNCWiki/Process+Help+and+Troubleshooti…>. Questions about individual tools should be made on the tool page. How do I add a page? Pages in Confluence are setup in a hierarchy, with a parent page capable of hosting multiple daughter pages. To create a new daughter page, go to the directory page that should serve as the parent page. Click the three dots next to the "Create" button to create a page from a template (currently available as Equipment and Process/Technology page templates), or click the "Create" button directly to create a new blank page. [cid:image009.jpg@01D3022D.DE3BC800] Where can I get more info? For more information, questions, or comments, email Justin Wirth<mailto:jcwirth@purdue.edu?subject=BNCWiki%20Question>. More information on using confluence can be found at the Purdue Confluence How-To Page<https://www.itap.purdue.edu/learning/tools-resources/confluence-essentials.…>, with all Confluence Online Documentation<https://confluence.atlassian.com/conf61/confluence-server-documentation-877…>. Important Grad Office Notices: Last week to make Birck look the best it can! Please look at each hallway to see the difference. July 24th - 28th West Wing 2nd floor hallway: Offices: BRK 2218, BRK 2222, BRK 2226, BRK 2230, BRK 2234 and BRK 2238 Seminars/Workshops NovaCentrix Seminar Dr. Rudresh Ghosh, Applications Engineer Tuesday, July 25 11:00 am - 12:00 pm BRK 2001 http://www.purdue.edu/discoverypark/birck/events/index.php Safe and Secure Systems and Software Symposium (S5) Air Force Research Laboratory (AFRL) August 1-3, 2017 http://www.mys5.org/index.php NSAC Undergraduate Research Symposium Summer 2017 Friday, August 2, 2017 2:00 - 4:00 p.m. Birck Atrium Register https://goo.gl/forms/vKZQ1U2Itg8dZyic2 by Friday, July 28. Toward Better Lives and Stronger Communities - Bringing the Pieces Together Jim McClelland, Executive Director for Drug Prevention, Treatment, and Enforcement for the State of Indiana Tuesday, September 12, 2017 1:30 - 2:30 p.m. Burton D. Morgan Center, Room 121 Reception at 2:30 -3:30, Mann Hall First Floor Atrium www.purdue.edu/discoverypark/dis<http://www.purdue.edu/discoverypark/dis> See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php Visitors to Birck Monday, July 24 through Thursday, July 27 LyoHUB Training 8:00 a.m. - 5:00 p.m. Tuesday, July 25 Dr. Rudresh Ghosh, Applications Engineer NovaCentrix Seminar OPFLEX Bob Hurly Mitch Stultz Wednesday, July 26 Tour by Sam Peana - Purdue Physics Research Experience for Undergraduates Program

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