- BNC-all - ecn.purdue.edu

Cell Culture Basics Training Workshop - June 14 & 15 2018 - 2nd Announcement
by Kwok, Tim 18 May '18

18 May '18

Dear Fellow Scientists, The 3D3C workshop on basic cell culture techniques is scheduled on June 14 & 15, 2018. Participation in the workshops requires having REM biosafety training and blood borne pathogen training. The course fee for the workshop is $250 (see flyer attached for more information). There is no prerequisite for the workshop. The registration deadline is May 25, 2018. If you are interested or have questions, please feel free to contact me. Thank you. Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/a033fa52-0bc…]

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Important News from the Business Office
by Turner, Jaime J 10 May '18

10 May '18

Hello Birck Family, There are a few important announcements regarding Transform Purdue and our summer schedule that I want to pass along. · Yesterday was the last day to enter NON-CATALOG orders into Ariba. If you would like to place a catalog order, you can do so until 4 PM on Thursday, May 31st. · Credit cards can be utilized for other purchases as necessary. Our credit card single purchase and cycle limits have been greatly increased to handle the heavier load while Ariba is down. With that said, the business office will be exercising caution when reviewing documentation and requests for credit cards. Requests to check a credit card out overnight will not be allowed. Special exceptions will be reviewed on a case by case basis. This will help to ensure that our cards do not get compromised. Please note: there is a spare computer in the business office so please do not be surprised if we ask you to place your order onsite so that the credit card does not have to be "checked-out". · The business office accounting systems will be converted to S4 HANA at the end of June. The system will go down Friday 6/29 and we do not expect it to come back up prior to July 5th. Please take that into account and be patient with us during that time. Once the conversion is complete, the account strings you are used to using today will be completely different. We will do our best to communicate those new account strings when we know what they are. I would also like to inform every one of the business office schedule for the summer months (5/14/18 - 8/17/18). · The business office will be open from 7:30 AM - 5 PM with the exception of a ½ hour from noon to 12:30 PM for lunch. This applies to every day of the work week, except Wednesday. As a gentle reminder, the business office staff often remain at their desks through the lunch period but are not working. Please be considerate of that. The rule of thumb is that if our doors are open, you are welcome to come in. If our doors our closed, the business office is closed. · The business office will be open all day, from 7:30 AM - 5 PM on Wednesdays. · Each member of the business office staff (excluding me) will be initiating a summer flex schedule so their normal weekly hours may vary from what they are now. They will be sending out their own communication to notify those that they work with closely. · Fiscal approvals for credit card orders will be limited on Friday afternoons from 3 PM - 5 PM. We ask that you take that into consideration when ordering. You will still have the availability to get approval but it may take additional time. The business office is very thankful that our Birck Family is always understanding of these sorts of things. As always, please do not hesitate to stop in and ask additional questions that you may have regarding Transform Purdue or the change to our office schedule for the summer. We want to be sure that you are fully aware of what is going on and when and how you can get ahold of us. ///////////////////////////////////////// Erica Cox Business Manager Birck Nanotechnology Center 1205 W. State Street West Lafayette, IN 47907 [New DP Logo B&G] * ericacox(a)purdue.edu<mailto:ericacox@purdue.edu> * 765-494-1619

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BNC E-news for the week of May 7th
by Turner, Jaime J 07 May '18

07 May '18

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Seminars/Workshops Dr. Ran Cheng Seminar Magnon Mediated Interlayer Coupling and Spin Transfer Torques May 11th @ 11:00am in MRGN 129 https://www.purdue.edu/discoverypark/birck/events/index.php?view=1529 See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php End of an Era Our Vistec VB6 electron beam lithography system was manufactured in 2000. In 2005/2006 it came to Purdue and was updated, installed and accepted in 2006. It has been in operation for 12 years now and has been a critical tool for the research here at Purdue and the Birck Nanotechnology Center. It will be available for research work for one more week, and then starting Monday May 14th, Vistec (Raith now) will be coming in to test various boards for one week. On Monday May 21st it will be fully de-commissioned and dismantling of it will start. It should take about a week to dismantle and ship parts back to Raith. It has served us well. The new JEOL 8100FS electron beam is its replacement. It is performing well and user training continues. Please see Justin Wirth or Bill Rowe for information & training on the JEOL 8100. Calling Discovery Park Undergraduate and Graduate Students, Are you interested in becoming a Discovery Park Ambassador? The Discovery Park Ambassador Program is an organized program supported by the Birck Nanotechnology Center, Bindley Bioscience Center and Discovery Park Administration. The mission of the program is to represent Discovery Park with the highest level of scientific integrity and professionalism at all engagement events and activities. The ambassadors' expected responsibilities include Discovery Park tours, engagement activities/events, K-12 workshops and industry engagement opportunities identified by the Engagement Coordinator. Ambassadors will be compensated accordingly by providing these important services to Discovery Park. If interested, please contact Nicole Finley at kingman(a)purdue.edu<mailto:kingman@purdue.edu>. [cid:image006.jpg@01D3E0AA.C60FC220] Hallway Floor Cleaning Schedule 5/8/2018 - BRK 2019 to BRK 2027 (offices closed until 10:00AM) 5/9/2018 - BRK 2287 to BRK 2295 (offices closed until 10:00AM) 5/10/2018 - The hallway on 2nd floor around stairway (Stairway and elevator closed until 10:00AM) 5/11/2018 - Area in front of cleanroom (you will still be able to access cleanroom from side hallways) 5/15/2018 -BRK 2262 to BRK 2283 (offices closed until 10:00AM) 5/16/2018 -West hallway in the main part of building. (BRK 2251, west stairwell, and elevator closed till 10:00AM) 5/17/2018 - BRK 2218 - BRK 2239 (offices closed until 10:00AM) 5/18/2018 - West hallway next to cleanroom (you must enter cleanroom on the west side will 10:00AM) 5/22/2018 - East hallway next to cleanroom (you must enter the cleanroom on the east side till 10AM) 5/23/2018 - East hallway 2nd floor (east stairwell, walkway to Bindley, and desk in east atrium, and 2nd floor lockers closed until 10:00AM. 5/24/2018 - BRK 2072 - BRK 2092 (offices closed until 10:00AM) 5/25/2018 - West Tour aisle by cleanroom (no freight elevator access until 10:00AM. (J- bay enter on east) 5/30/2018 - BRK 2031 to BRK 2050 (offices closed until 10:00AM) ALL CLOSURES ON THIS LIST ARE FROM 5:00am TO 10:00am Aspire Four Week Look Ahead Schedule Attached is the Aspire Four Week Look Ahead Schedule. It is the projection of vibration and/or noise issues with the construction outside of Birck.

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Cell Culture Basics Training Workshop - June 14 & 15 2018
by Kwok, Tim 07 May '18

07 May '18

Dear Fellow Scientists, The 3D3C workshop on basic cell culture techniques is scheduled on June 14 & 15, 2018. Participation in the workshops requires having REM biosafety training and blood borne pathogen training. The course fee for the workshop is $250 (see flyer attached for more information). There is no prerequisite for the workshop. The registration deadline is May 25, 2018. If you are interested or have questions, please feel free to contact me. Thank you. Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/a033fa52-0bc…]

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BNC E-news for the week of April 30th
by Turner, Jaime J 30 Apr '18

30 Apr '18

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Seminars/Workshops Gregory Sotzing Seminar Organic Conductive Polymers for Wearable Electronics May 2nd @ 11:00am in BRK 1001 https://www.purdue.edu/discoverypark/birck/events/index.php?view=1509 PQC distinguished Seminar - Dirk Englund Seminar Semiconductor Quantum Technologies for Secure Communications and Scalable Quantum Networks May 4th @ 2:00pm in BRK 2001 https://www.purdue.edu/discoverypark/birck/events/index.php?view=1507 See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php Birck Nanotechnology Center PM Shut Down Notice The Birck Nanotechnology Center will be undergoing a semi-annual preventive maintenance and service shutdown April 30 through May 4, 2018. Air handling systems, exhaust systems, toxic gas delivery, and monitoring systems will be down beginning at 7 a.m. Monday, April 30 through 4 p.m. Friday, May 4. The BNC staff will be performing appropriate preventive maintenance on the process equipment at this time as well. All CLEANROOM research work will be suspended until the maintenance activities are completed and all systems are returned to normal. Only authorized personnel will be allowed to enter the cleanroom during this time period. In addition, the laboratory areas will also be affected by this systems maintenance. As such, all LABORATORY activities that involve exhausted hoods, vacuum systems, and/or toxic gas systems will be down beginning 7 a.m. Monday, April 30 through 4 p.m. Friday, May 4. However, work that involves taking standard electrical measurements may be carried out in the LABS provided that fluctuating air temperature and humidity do not affect the readings. No chemical work will be allowed during this period. It is anticipated that the building and process tool maintenance will be completed by the end of the day on Friday, May 4. Please check iLabs for equipment status and availability. For long term research planning please refer the following future dates for our future preventive maintenance shutdowns. Week of Oct 15, 2018 Week of April 29, 2019 Week of Oct 14, 2019 Spring May 4, 2020 Thank you for your cooperation and understanding during these important preventive maintenance shutdowns. Mark Voorhis Building Manager Birck Nanotechnology Center Calling Discovery Park Undergraduate and Graduate Students, Are you interested in becoming a Discovery Park Ambassador? The Discovery Park Ambassador Program is an organized program supported by the Birck Nanotechnology Center, Bindley Bioscience Center and Discovery Park Administration. The mission of the program is to represent Discovery Park with the highest level of scientific integrity and professionalism at all engagement events and activities. The ambassadors' expected responsibilities include Discovery Park tours, engagement activities/events, K-12 workshops and industry engagement opportunities identified by the Engagement Coordinator. Ambassadors will be compensated accordingly by providing these important services to Discovery Park. If interested, please contact Nicole Finley at kingman(a)purdue.edu<mailto:kingman@purdue.edu>. [cid:image006.jpg@01D3E0AA.C60FC220] Hallway Floor Cleaning Schedule 5/8/2018 - BRK 2019 to BRK 2027 (offices closed until 10:00AM) 5/9/2018 - BRK 2287 to BRK 2295 (offices closed until 10:00AM) 5/10/2018 - The hallway on 2nd floor around stairway (Stairway and elevator closed until 10:00AM) 5/11/2018 - Area in front of cleanroom (you will still be able to access cleanroom from side hallways) 5/15/2018 -BRK 2262 to BRK 2283 (offices closed until 10:00AM) 5/16/2018 -West hallway in the main part of building. (BRK 2251, west stairwell, and elevator closed till 10:00AM) 5/17/2018 - BRK 2218 - BRK 2239 (offices closed until 10:00AM) 5/18/2018 - West hallway next to cleanroom (you must enter cleanroom on the west side will 10:00AM) 5/22/2018 - East hallway next to cleanroom (you must enter the cleanroom on the east side till 10AM) 5/23/2018 - East hallway 2nd floor (east stairwell, walkway to Bindley, and desk in east atrium, and 2nd floor lockers closed until 10:00AM. 5/24/2018 - BRK 2072 - BRK 2092 (offices closed until 10:00AM) 5/25/2018 - West Tour aisle by cleanroom (no freight elevator access until 10:00AM. (J- bay enter on east) 5/30/2018 - BRK 2031 to BRK 2050 (offices closed until 10:00AM) ALL CLOSURES ON THIS LIST ARE FROM 5:00am TO 10:00am Aspire Four Week Look Ahead Schedule Attached is the Aspire Four Week Look Ahead Schedule. It is the projection of vibration and/or noise issues with the construction outside of Birck.

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NanoDays 2018 Recap
by Weinstein, Dana 27 Apr '18

27 Apr '18

Dear all, NanoDays 2018<https://www.purdue.edu/discoverypark/birck/events/NanoDays/index.php> was a great opportunity for all of us to help impact a lot of lives. Over 150 individuals and groups worked hard to make this event a big success! I would like to express a big thank you to everyone for contributing to this important event! As I am sure you have personally experienced, it makes a big difference for many kids and their families. A special thank you goes to Ron Reger & Jaime Turner who chaired and led the event, and the Nanodays Committee members: Sarah Anderson, Neil Dilley, Sajid Choudhury, Nicole Finley,, Tim Miller, Joon Park, Sean Rinehart, and Justin Wirth. This year we had over 900 Pre-K through high school students participate in NanoDays, and over 130 volunteers who made it a successful STEM event for the next generation of scientists! In addition to the traditional NanoDays demonstrations and experiments we had research booths showing a number of Purdue research groups and other technologies: 1) Peroulis Group 2) OSA/SPIE 3) Daniel Suter group, nanobiology 4) Liang Pan group, optics demos 5) Alex Wei group, Au nanoparticles 6) Jong Hyun Choi group, nanoparticles 7) NanoHUB, build and model nanotubes 8) Vlad Shalaev group 9) Tongcang Li Group 10) Joerg Appenzeller group, graphite racers and nanomagnetism We would also like to thank NSAC for their dedication in helping with Nanodays year after year and running of the mock cleanroom! On behalf of the Birck Center, we wish to express our sincere thanks to all of the volunteers for making NanoDays 2018 a huge success! With sincere appreciation, Dana Weinstein Associate Director of Operations, BNC ——————————————— ——————————————— Dana Weinstein Associate Professor, Electrical and Computer Engineering Associate Director of Operations, Birck Nanotechnology Center, Discovery Park Purdue University https://engineering.purdue.edu/hybridmems [cid:D3B48EC2-DBE7-4D92-B8CE-2FC8DF5C7026@lfytina2.metronetinc.com]

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Seminar - Venkatesh Narayanamurti - Tuesday, April 24th, Burton D. Morgan Center, Room 121
by Shakouri, Ali 23 Apr '18

23 Apr '18

Dear All, I encourage you to attend the seminar by Prof. Venky Narayanamurti from Harvard tomorrow, Tuesday at 1:30pm at Burton Morgan Center 121. Venky is a former director of research at Bell Labs, vice president at Sandia and Dean of Engineering at UCSB and at Harvard. His current work, at the Kennedy School of Public Policy, focuses on innovation and ecosystems that promote invention and discovery. Best regards, Ali Dear Colleagues, On behalf of Discovery Park, please join us on, Tuesday, April 24th at 1:30pm at the Burton D. Morgan Center, Room 121 to hear Discovery Park Distinguished Lecture Series seminar speaker, Venkatesh Narayanamurti present the talk entitled, Bridging The Basic-Applied Dichotomy and The Cycles of Invention and Discovery. Venkatesh Narayanamurti is the Benjamin Peirce Research Professor of Technology and Public Policy at Harvard. We hope to see you there. Cheers, Tomás Díaz de la Rubia [cid:image002.jpg@01D3D725.B0C88750]

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BNC E-news for the week of April 23rd
by Turner, Jaime J 20 Apr '18

20 Apr '18

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Seminars/Workshops Jonathan Fan Seminar High Performance Meta-optical Systems for Extreme Waveform Control April 25th @ 11:00am in BRK 1001 https://www.purdue.edu/discoverypark/birck/events/index.php?view=1506 Gregory Sotzing Seminar Organic Conductive Polymers for Wearable Electronics May 2nd @ 11:00am in BRK 1001 https://www.purdue.edu/discoverypark/birck/events/index.php?view=1509 PQC distinguished Seminar - Dirk Englund Seminar Semiconductor Quantum Technologies for Secure Communications and Scalable Quantum Networks May 4th @ 2:00pm in BRK 2001 https://www.purdue.edu/discoverypark/birck/events/index.php?view=1507 See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php Birck Nanotechnology Center PM Shut Down Notice The Birck Nanotechnology Center will be undergoing a semi-annual preventive maintenance and service shutdown April 30 through May 4, 2018. Air handling systems, exhaust systems, toxic gas delivery, and monitoring systems will be down beginning at 7 a.m. Monday, April 30 through 4 p.m. Friday, May 4. The BNC staff will be performing appropriate preventive maintenance on the process equipment at this time as well. All CLEANROOM research work will be suspended until the maintenance activities are completed and all systems are returned to normal. Only authorized personnel will be allowed to enter the cleanroom during this time period. In addition, the laboratory areas will also be affected by this systems maintenance. As such, all LABORATORY activities that involve exhausted hoods, vacuum systems, and/or toxic gas systems will be down beginning 7 a.m. Monday, April 30 through 4 p.m. Friday, May 4. However, work that involves taking standard electrical measurements may be carried out in the LABS provided that fluctuating air temperature and humidity do not affect the readings. No chemical work will be allowed during this period. It is anticipated that the building and process tool maintenance will be completed by the end of the day on Friday, May 4. Please check iLabs for equipment status and availability. For long term research planning please refer the following future dates for our future preventive maintenance shutdowns. Week of Oct 15, 2018 Week of April 29, 2019 Week of Oct 14, 2019 Spring May 4, 2020 Thank you for your cooperation and understanding during these important preventive maintenance shutdowns. Mark Voorhis Building Manager Birck Nanotechnology Center Do not store items under desks I would like you to please refrain from storing your items under your desk. Our custodial crew needs to keep the floors clean and are not allowed to touch any personal items. Could you please Use the shelves above your desk to store these items? If you have any questions, please call me at 6-1173. Mary Jo Kitchen Refrigerator Clean-out Notice: The kitchen refrigerator will be cleaned out on Wednesday April 25 at 2PM At that time anything that is not labeled and/or looks bad to me will be trashed. Container and all. Please label your food if you do not want it trashed. Thank you Nancy Black Secretary V Birck Nanotechnology Center West Lafayette, IN 47907-2057 Phone: 765-494-8675 Fax: 765-494-4731 Aspire Four Week Look Ahead Schedule Attached is the Aspire Four Week Look Ahead Schedule. It is the projection of vibration and/or noise issues with the construction outside of Birck.

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BNC E-news for the week of April 2nd
by Turner, Jaime J 02 Apr '18

02 Apr '18

[BIRCK NANOTECHNOLOGY CENTER][http://www.purdue.edu/discoverypark/resources/toolkit/files/logo/di…] Seminars/Workshops Matthew L. Becker Seminar Building bone with polymers- How new materials and additive manufacturing are changing medicine April 5th @ 2:00pm in BRK 1001 http://www.purdue.edu/discoverypark/birck/events/index.php?view=1420 Zhenan Bao Seminar Designing Skin-Inspired Organic Electronic Materials April 13th @ 11:00am in STEW 218 Michael Chabinyc Seminar Energy Conversion with Organic Semiconductors April 18th @ 11:00am in BRK 1001 http://www.purdue.edu/discoverypark/birck/events/index.php?view=1427 See other Discovery Park's Events: http://www.purdue.edu/discoverypark/events/index.php Birck Nanotechnology Center PM Shut Down Notice The Birck Nanotechnology Center will be undergoing a semi-annual preventive maintenance and service shutdown April 30 through May 4, 2018. Air handling systems, exhaust systems, toxic gas delivery, and monitoring systems will be down beginning at 7 a.m. Monday, April 30 through 4 p.m. Friday, May 4. The BNC staff will be performing appropriate preventive maintenance on the process equipment at this time as well. All CLEANROOM research work will be suspended until the maintenance activities are completed and all systems are returned to normal. Only authorized personnel will be allowed to enter the cleanroom during this time period. In addition, the laboratory areas will also be affected by this systems maintenance. As such, all LABORATORY activities that involve exhausted hoods, vacuum systems, and/or toxic gas systems will be down beginning 7 a.m. Monday, April 30 through 4 p.m. Friday, May 4. However, work that involves taking standard electrical measurements may be carried out in the LABS provided that fluctuating air temperature and humidity do not affect the readings. No chemical work will be allowed during this period. It is anticipated that the building and process tool maintenance will be completed by the end of the day on Friday, May 4. Please check iLabs for equipment status and availability. For long term research planning please refer the following future dates for our future preventive maintenance shutdowns. Week of Oct 15, 2018 Week of April 29, 2019 Week of Oct 14, 2019 Spring May 4, 2020 Thank you for your cooperation and understanding during these important preventive maintenance shutdowns. Mark Voorhis Building Manager Birck Nanotechnology Center Nanodays 2018 Volunteer Sign-Up Purdue's Birck Nanotechnology Center has an exciting volunteer opportunity for students in the technical, education, and management fields. Participants will have the opportunity to: * Gain professional experience explaining scientific concepts to people from non-technical backgrounds. * Support the future of STEM programs by introducing grade schoolers and high school students to the miniscule world of atoms, molecules, and nanoscale forces. * Participate with other students/researchers in a nationwide festival celebrating nanotechnology science and education. Since 2010, the Birck Nanotechnology Center at Discovery Park has participated in NISE NanoDays, a program that promotes science and technology education nationwide. Volunteers will staff activity stations and assist visitors as they investigate different scientific concepts. Some example activities for 2018 include gummy capsules, ferrofluids, invisibility, thin film bookmarks, oobleck, and UV bead bracelets. No prior knowledge is required to volunteer, just an enthusiasm for science and science education. Local vendors will be providing food for volunteer training and all three days of the event. Volunteers can attend any or all of the following shifts: Wed 4/11/18: 5:30 PM - 8:00 PM Thu 4/12/18: 8:45 AM - 11:30 AM 11:15 AM - 2:00 PM Fri 4/13/18: 8:45 AM - 11:30 AM 11:15 AM - 2:00 PM Training is mandatory and will be provided in the Birck atrium. The schedule is: Tue 4/03/18: 5:00 PM - 5:30 PM (Pizza Party) 5:30 PM - 6:30 PM (Training Sessions) Interested volunteers can sign up here: https://tinyurl.com/ycnov92z A full activity list can be found here: https://tinyurl.com/yacebhpu Questions can be directed to volunteer coordinators Joon Park (park218(a)purdue.edu<mailto:park218@purdue.edu>), Justin Wirth (jcwirth(a)purdue.edu<mailto:jcwirth@purdue.edu>), and Sean Rinehart (srinehar(a)purdue.edu<mailto:srinehar@purdue.edu>). Important Procurement Transition Process We have been communicating for some time that Purdue is undergoing a campus wide transformation process related to many aspects of our daily work life. The finance module of this project is set to "go-live" on July 1st, 2018. This transformation will redesign the core financial structure to a standardized structure that allows us to truly utilize SAP. In order to prepare for this transition, there will be a period of time where we will not be allowed to place orders through Ariba. IMPORTANT DATES: * April 18th - Any orders greater than $10K have to be submitted and approved in Ariba * May 9th - Final day for ALL OTHER Ariba orders to be submitted - "requester role" will be removed from all who have access * May 10th - July 1st: * P-cards will be used for purchases that are considered low-risk/small transactions. Our credit card limits will be increased to account for the increase in transactions. * Direct Payments can be used when a vendor will not accept credit cards during this time frame. However, direct payment submitted after June 1st will not be submitted to the vendor until AFTER July 1st. * For high risk/capital equipment a form 12 will need to be submitted to the procurement help desk so that the appropriate authorizations can be obtained via DocuSign. BEST PRACTICES FOR TRANSITION PERIOD: * If you plan on ordering a piece of capital equipment, please obtain all necessary documentation and approvals and enter the PO no later than 4/18. * If you routinely purchase certain items or have specific orders scheduled for May or June, please place those orders now. * Long-term or multi-year projects should be broken down into multiple POs with the first phase anything that would need paid out prior to the end of June. * If you have a situation that you are unsure of, please discuss with the business office. If you have ANY questions or concerns, please let me know. Thank you all for your understanding on this matter. ///////////////////////////////////////// Erica Cox Business Manager Birck Nanotechnology Center 1205 W. State Street West Lafayette, IN 47907 [New DP Logo B&G] * ericacox(a)purdue.edu<mailto:ericacox@purdue.edu> ( 765-494-1619 Skywalk Closed for use April 17th and 18th On Tuesday April 17th and Wednesday April 18th we will be stripping and waxing the floor in the skywalk between Bindley and Birck. Both days the skywalk will be unavailable for use from 5:00AM - 10:00AM. Please make plans to use an alternative route. Thank you for your understanding as we work hard to maintain the beauty of our buildings. Thanks, Mary Jo Aspire Four Week Look Ahead Schedule Attached is the Aspire Four Week Look Ahead Schedule. It is the projection of vibration and/or noise issues with the construction outside of Birck.

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3D3C Newsletter - February 2018
by Kwok, Tim 27 Mar '18

27 Mar '18

Dear All, Below please find the fourteenth issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center. The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news) In this issue, a new section, "3D in action", is added. The five sections in the newsletter are: 3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue 3D in action - this section highlights recent activity in 3D3C 3D in focus – this section presents the current work on a specific 3D cell culture model or technique 3D in publications – this section brings a collection of recent publications on 3D cell culture 3D in meetings – this section includes a list of upcoming meetings related to 3D cell culture The newsletter will be available every two months. If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe. Please contact me if you have questions. Yours Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/3e8e050f-67f…] Volume 14, February 2018 [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/10f15e72-d4d…] 3D at Purdue 3D in Action 3D in Focus 3D in Publications 3D in Meetings 3D at Purdue Cell-instructive Biomaterials for Musculoskeletal Soft Tissue Regenerative Engineering Regenerative engineering represents a new transdisciplinary paradigm to generate functional tissues and tissue systems based on Convergence of Advanced Materials Science, Stem Cell Science, Physics, Developmental Biology, and Clinical Translation. In particular, biomaterials provide an enabling technology platform to engineer a three-dimensional (3D) scaffold system offering a supportive microenvironment to direct cell migration, growth, differentiation, and organization to form regenerated tissue. The development of 3D scaffolds that mimic the hierarchical architecture of native tissue extracellular matrix requires a fundamental understanding of the structure-function relationship and cell-biomaterial interactions. The Deng Laboratory focuses on both the fundamental understanding of cellular processes in tissue development and engineering and effective biomaterial systems for musculoskeletal regenerative engineering. Our strategies for tissue regeneration embrace the mechanistic understanding of various physicochemical and biological cues on cellular processes, and the application of advanced biomaterials and matrix technologies at the micro- and nanoscale (Figure 1). [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/49792c75-b18…] Figure 1. Design criteria for different material cues on cell-material interactions. Physical cues, such as material topography, stiffness, and porosity, can dramatically affect cell fate and tissue development. Incorporation of chemical cues in the form of simple chemical groups into materials influences cell behavior. Additionally, material carrier presentation of various biological cues such as growth factors and small molecules/inducerons can lead to enhanced cellular responses. Integrating these cues is paramount in creating a synthetic matrix optimized for desirable cellular responses and inductive tissue regeneration (Narayanan, N., Jiang, C., Uzunalli, G., Shalumon, K.T., Laurencin, C.T., Deng, M.: Regenerative Engineering and Translational Medicine. 2016, 2(2):69-84). In our recent collaborative work with Dr. Shihuan Kuang in Animal Sciences, we have developed a bioengineered cell niche composed of aligned polyester fiber scaffolds for skeletal muscle regeneration. Current cell-based therapies for skeletal muscle regeneration are hindered by low survival and long-term engraftment of the transplanted cells due to the lack of appropriate supportive microenvironment (cell niche) in the injured muscle. Therefore, there is a critical need for developing strategies that can provide cellular and structural support for the regeneration of new functional skeletal muscles. Inspired by the native architecture of skeletal muscle, we have created aligned polyester fiber scaffolds mimicking the oriented organization of skeletal muscle by electrospinning using a novel custom-made rotating collector made of parallel metal blades. Aligned fibers with diameters varying from 335 ± 154 nm (nanoscale) to 3013 ± 531 nm (microscale) were fabricated by varying the poly(lactide-co-glycolide) (PLGA) concentration. Myoblasts seeded on aligned PLGA fibers exhibited oriented arrangement according to the direction of fiber alignment resulting from contact guidance (Figure 2). Interestingly, microfiber scaffolds enhanced cell alignment, elongation, proliferation, and differentiation as compared to the nanofiber scaffolds indicating the ability of cells to respond to fiber topography. Pilot in vivo studies were performed to evaluate the regenerative potential of the scaffolds using a dystrophin-deficient mdx mouse model. Following 21 days of implantation, microfiber scaffolds seeded with primary myoblasts resulted in the formation of a network of dystrophin positive myofibers in tibialis anterior muscles of mdx mice. These experiments not only provide critical insights on exploiting the interactions between muscle cells and their microenvironment, but also open new avenues for the development of effective muscle regenerative technologies. [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/d8a03b2d-de3…] Figure 2. Fluorescence image of myoblasts on aligned fibers after 24 hours of cell seeding. Cells spread along the direction of fiber orientation on aligned scaffolds (Red: cytoskeletal actin; Blue: nuclei) Relevant Publications: Kumbar, S.G., Laurencin, C.T., Deng, M (Editors): Natural and Synthetic Biomedical Polymers, Elsevier, ISBN: 978-0-12-396983-5, 2014. Narayanan, N., Jiang, C., Uzunalli, G., Shalumon, K.T., Laurencin, C.T., Deng, M.: Polymeric electrospinning for musculoskeletal regenerative engineering. Regenerative Engineering and Translational Medicine. 2016, 2(2):69-84. Funding support from NIH R03AR068108 and Purdue Start-up Package is greatly appreciated. Meng Deng Assistant Professor Department of Agricultural & Biological Engineering ________________________________ 3D in Action Workshop Invitation: 3D3C is invited to organize a workshop for Experimental Biology 2018 Annual Meeting. The workshop is scheduled on April 22, 2018. The title for the workshop is, "The Art of 3D Cell Culture, from Organoids to Organ-on-A-Chip" Recent publication from 3D3C: Lelièvre SA, Kwok T, Chittiboyina S. Architecture in 3D cell culture: An essential feature for in vitro toxicology Toxicol In Vitro. 2017 Dec;45(Pt 3):287-295 Abstract Three-dimensional cell culture has the potential to revolutionize toxicology studies by allowing human-based reproduction of essential elements of organs. Beyond the study of toxicants on the most susceptible organs such as liver, kidney, skin, lung, gastrointestinal tract, testis, heart and brain, carcinogenesis research will also greatly benefit from 3D cell culture models representing any normal tissue. No tissue function can be suitably reproduced without the appropriate tissue architecture whether mimicking acini, ducts or tubes, sheets of cells or more complex cellular organizations like hepatic cords. In this review, we illustrate the fundamental characteristics of polarity that is an essential architectural feature of organs for which different 3D cell culture models are available for toxicology studies in vitro. The value of tissue polarity for the development of more accurate carcinogenesis studies is also exemplified, and the concept of using extracellular gradients of gaseous or chemical substances produced with microfluidics in 3D cell culture is discussed. Indeed such gradients-on-a-chip might bring unprecedented information to better determine permissible exposure levels. Finally, the impact of tissue architecture, established via cell-matrix interactions, on the cell nucleus is emphasized in light of the importance in toxicology of morphological and epigenetic alterations of this organelle. ________________________________ 3D in Focus The architectural and mechanical properties of a scaffold can affect cell behaviors, such as spreading, proliferation and differentiation. Stiffness, a key mechanical property, was studied mostly in systems with a single cell type. Each cell type responds to matrix stiffness in its own way and may affect the other cell types within the same biological system. Subjecting macrophages (Mφ) and bone marrow-derived mesenchymal stem cells (BMMSCs) to a stiffness-tunable matrix, the authors in the highlighted research article investigated how matrix stiffness may regulate the effect of Mφ on osteogenesis of BMMSCS. Xiao-Tao He, Rui-Xin Wu, Xin-Yue Xu, Jia Wang, Yuan Yin, Fa-Ming Chen. Macrophage involvement affects matrix stiffness-related influences on cell osteogenesis under three-dimensional culture conditions. Acta Biomaterialia https://doi.org/10.1016/j.actbio.2018.02.015 3D3C summary of the article: Transglutaminase cross-linked gelatin (TG-gel) with different stiffness levels were prepared by crosslinking different percentages of gelatin with transglutaminase, 3% (Low), 6% (Mid) and 9% (High). The stiffness of TG-gel increased and the porosity decreased with an increase in the percentage of gelatin. Both proliferation and viability of BMMSCs were higher in low-stiffness TG-gels than in high-stiffness TG-gel. The osteogenic potential of the BMMSCs, assessed by Alizarin Red S staining of mineralized nodules, release of alkaline phosphatase (ALP), and the expression levels of osteogenic genes (SP7, OST, RUNX2, ALP and OCN) were elevated with an increase in matrix stiffness. In response to microenvironmental signals, Mφ undergo polarization into distinct functional cells, M1, M2 cells. The polarization status of the Mφ in matrices with different stiffness levels were analyzed via qRT-PCR, immunofluorescence and ELISA. The expression levels of M1-related markers (IL-1β and TNF-α) increased proportionally with an increase in stiffness of the TG-gel. Mφ effects on osteogenesis of BMMSCs cultured in different stiffness levels of TG-gel were examined in a Transwell co-culture system, in which gel-encapsulated Mφ were placed in the upper compartment, and the BMMSCs were in the bottom compartment. The ALP activity and the formation of mineralized nodules in non-encapsulated monolayers of BMMSCs were enhanced when these cells were incubated with Mφ encapsulated in low-stiffness gel compared to high-stiffness gel. Mφ encapsulated in low-stiffness TG-gel increased the formation of mineralized nodules and the ALP activity in BMMSCs encapsulated in either low- or high-stiffness TG-gels, whereas Mφ in high-stiffness TG-gel suppressed these phenomena. By encapsulating Mφ (in the upper compartment) and BMMSCs (in the bottom compartment) in TG-gel of the same stiffness level, the BMMSCs in low- and high-TG-gel displayed higher ALP activity and mineralized nodules formation than the BMMSCSs in mid-stiffness TG-gel, suggesting the nonlinear influence of stiffness for the effect of on BMMSCs osteogenesis. Comments from 3D3C: Extracellular matrix stiffness has profound impacts on cell function and differentiation. Most of the studies related to stiffness are performed, however, on culture systems with a single cell type, while multiple cell types are present in vivo. By encapsulating BMMSCs and Mφ in TG-gel with different stiffness levels in separate chambers of a Transwell system, the studies presented here argue against the conventional rationale that data obtained from a study with a single cell type reflect what happens in vivo where multiple cell types are present. In addition, the possibility that stiffness might vary locally (e.g., next to a particular cell type compared to another cell type) in vivo should also be explored, as results here suggested that the result of the interaction between different cell types might depend on their respective environmental stiffness. An important aspect to explore in future experiment is how the cells would influence each other if placed in the same culture, a system that would better mimics in vivo conditions. ________________________________ 3D in Publications Recent publications on 3D culture (please click to access the list on our web page https://nanohub.org/groups/3d3cfacility) Review<https://nanohub.org/groups/3d3cfacility/news#reviews> The research articles and reviews are arranged in the following categories: Scaffold free/Scaffold Organ/Tissue/Cell Others Spheroids<https://nanohub.org/groups/3d3cfacility/news#spheroids> Organoid<https://nanohub.org/groups/3d3cfacility/news#organoid> Scaffold<https://nanohub.org/groups/3d3cfacility/news#scaffold> Hydrogel<https://nanohub.org/groups/3d3cfacility/news#hydrogel> Matrix<https://nanohub.org/groups/3d3cfacility/news#matrix> Microfluidics<https://nanohub.org/groups/3d3cfacility/news#microfluidic> Microfabrication<https://nanohub.org/groups/3d3cfacility/news#microfabrication> Bone<https://nanohub.org/groups/3d3cfacility/news#bone> Bone Marrow<https://nanohub.org/groups/3d3cfacility/news#bonemarrow> Breast<https://nanohub.org/groups/3d3cfacility/news#breast> Colon<https://nanohub.org/groups/3d3cfacility/news#colon> Heart<https://nanohub.org/groups/3d3cfacility/news#heart> Liver<https://nanohub.org/groups/3d3cfacility/news#liver> Lung<https://nanohub.org/groups/3d3cfacility/news#lung> Muscle<https://nanohub.org/groups/3d3cfacility/news#muscle> Nerve<https://nanohub.org/groups/3d3cfacility/news#nerve> Prostate<https://nanohub.org/groups/3d3cfacility/news#prostate> Endothelial cells<https://nanohub.org/groups/3d3cfacility/news#endothelialcells> Fibroblast<https://nanohub.org/groups/3d3cfacility/news#fibroblast> Stem Cells<https://nanohub.org/groups/3d3cfacility/news#stemcells> Stromal Cells<https://nanohub.org/groups/3d3cfacility/news#stromalcells> Cancer/Tumor<https://nanohub.org/groups/3d3cfacility/news#cancer> Screening<https://nanohub.org/groups/3d3cfacility/news#screening> 3D bioprinting<https://nanohub.org/groups/3d3cfacility/news#3dbioprinting> Imaging<https://nanohub.org/groups/3d3cfacility/news#imaging> ________________________________ 3D in Meetings Keystone Symposia: Organs- and Tissues-on-Chips Date: 8th to 12th April 2018 Location: Big Sky, Montana, USA Website: http://www.keystonesymposia.org/18D1 Contact person:info@keystonesymposia.org Organized by: Keystone Symposia 3rd International Conference on Nanomedicine, Drug Delivery, and Tissue Engineering (NDDTE'18) Date: 10th to 12th April 2018 Location: New Delhi, India Website: http://nddte.com/ Contact person: Conference Administrator Organized by: International ASET 3rd International Conference on 3D Printing in Medicine Date: 4th to 5th May 2018 Location: Halle, Germany Website: http://3dprint-congress.com/ Contact person:congress@bb-mc.com Organized by: boeld communication GmbH 2nd World Congress and Expo on Nanotechnology and Materials Science Date: 25th to 27th June 2018 Location: Dubai, United Arab Emirates Website: https://biocoreconferences.com/nanotechnology2018/index.php Contact person: Hany Organized by: Biocore 4th International Conference on Bio-based Polymers and Composites Date: 2nd to 6th September 2018 Location: Balatonfüred, Hungary Website: http://www.bipoco2018.hu Contact person: Dóra Tátraaljai Bio-based polymers and their blends, composites. Natural polymers and their modification. Natural fiber reinforced composites. Other raw materials based on natural resources. Biodegradation and environmental issues EMBO|EMBL Symposium: Organoids: Modelling Organ Development and Disease in 3D Culture Date: 10th to 13th September 2018 Location: Heidelberg, Germany Website: http://www.embo-embl-symposia.org/symposia/2018/EES18-08/index.html Contact person: events(a)embl.de<mailto:events@embl.de> Organized by: EMBL

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