All,
Due to the variety of operating conditions required for HPLC system #2
using the C-18 column we have devised a system to provide fair and
efficient operation to all users.We will alternate the system operating
conditions on a weekly basis.We will set up the system for the following
week on Friday, which will allow the system to equilibrate over the
weekend and be ready to run on Monday.Let us know if you need us to
modify the schedule and we would be happy to accommodate your needs, but
the goal is to avoid changing the column and mobile phase every
day.While the operating conditions were designed for each experiment,
you are welcome to run samples whenever you want.Here is our tentative
schedule:
_Week of 5/27: C_3 Bio program_
C18 column (Agilent, XDB-C18, 4.6x50 mm, 1.8 micrometers), UV detector,
and 2 mobile phases: 1) 0.1% Formic Acid in DI H20 and 2) 100% methanol
_Week of 6/3: Fernanda Marisa da Cunha's Feruloyl Esterase Assay_
C18 column (EC 250/4.6 NUCLEOSIL 100-7 C18), UV detector, and two mobile
phases: 1) 50 mL methanol and .25 mL acetic acid per L water and 2) .25
mL acetic acid per L methanol
_Week of 6/10: He Shang-Guessmix Compounds for HPLC Analysis_
C18 column (EC 250/4.6 NUCLEOSIL 100-7 C18), UV detector, and methanol
and H2O mobile phase
At this point we will plan on repeating this cycle, but if there is a
better method of sharing the column we will modify our strategy.
Thank you,
LORRE HPLC Recharge Center
