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Announcements: Data Mngt session today; Poll for Summer Events; New Publication from Ca++ Journal Club
by Brent Thomas Ladd 01 Dec '25

01 Dec '25

[cid:d1a9c962-22f6-4103-9fcc-65a1f5f77d7a] Dear EMBRIOphytes, I hope everyone was able to enjoy the holiday break. I had the pleasure of taking my sister and her family (who reside in Florida) to the frigid ❄️ Friday night Purdue vs. Indiana football game... to ensure no one would lose a toe or finger we vacated ⛷ after the half-time show ⛄ . Announcements include a reminder to invited labs to attend today's All-Hands data management session, a poll for your input on summer events, and a highlight of the awesome review paper in APL Bioengineering co-authored by members of the Calcium Signaling Journal Club: Today 3pm Data Management Session for Invited Labs Today's EMBRIO All-Hands Lab is dedicated to meeting with specific invited computational and experimental labs on data management tasks and needs. All are welcome, though if you received an email from me last week about attending this session, please do tune in - those who have a time conflict we can get with you another time before the semester ends. Computational labs will likely take 15 minutes or less once we cover GitHub and PURR, and the few experimental labs invited we will use the balance of time to address any imaging data tasks and needs going forward. We Need Your Input: Dates, Plans, Topics for EMBRIO 2026 Summer School & Joint Symposium A joint EMBRIO BII NSF - Notre Dame (RECODE NSF) one day symposium and poster session has been proposed focused on "Advancing Morphogenetic Biosciences and Bioengineering”, coupled with a multi-day training at the intersection of AI-levered computational modeling, quantitative biology, and bioengineering (e.g. https://sai.nd.edu/<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fsai.nd.ed…>). The Symposium will feature EMBRIO speakers, along with speakers from Notre Dame, and invited Key Notes. Proposed to be hosted at our partners at University of Notre Dame, South Bend, Indiana, EMBRIO will organize and help provide transportation and lodging for Purdue members to attend, as well as reimburse travel for EMBRIO trainees from our partner institutions to participate. Please use this brief online poll to capture your availability, plans and preferences, as well as ideas for training topics: https://purdue.ca1.qualtrics.com/jfe/form/SV_bkIw8bHUboD9Qwu<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> New EMBRIO Paper in APL Bioengineering! This integrative review exploring the “Rules of Life” at the interface of Calcium Signaling and Mechanobiology involved nine EMBRIO labs spanning all of our biological thrust areas and the Core Thrust! This work focuses on how Ca2+ regulates and is regulated by cytoskeletal dynamics, with a particular emphasis on computational modeling for predictive simulations. In exploring a Rules of Life (RoL) perspective, the team defined and reviewed the multi-scalar principles governing the relationships between calcium signaling and cellular mechanics and organization: * RoL 1: Ca2+ dynamics facilitate cytoskeletal reorganization following stress and damage. * RoL 2: Ca2+ regulates actin dynamics to control synapse processes supporting both synapse formation and exocytosis. * RoL 3: Reciprocal coupling of spatiotemporal Ca2+ signaling and cellular dynamics defines distinct cellular roles in emergent multicellular behavior. 🔗 Link to Paper: https://doi.org/10.1063/5.0284022<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> Congrats to the entire team for this major accomplishment! Linlin Li and David Gazzo contributed equally to this work as co-first authors, while Shams Mowafak Saad, Nissa Larson, Eugene Kim, and Nilay Kumar contributed equally as co-second authors. Jeremiah Zartman, as the senior author, organized and supervised the Calcium Signaling Journal Club that led to this exciting publication. Li, L., Gazzo, D., Saad, S., Larson, N., Kim, E., Kumar, N., Mim, M., Krishnan, M., Speybroeck, B., Ding, C., Mullins, M., Pascuzzi, A., Deng, Q., Pienaar, E., Evans, J., Umulis, D., Zartman, J. Rules of Life at the Interface of Calcium Signaling and Mechanobiology. Open Access November 26, 2025. APL Bioengineering. https://doi.org/10.1063/5.0284022<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> [Calcium signaling and actin dynamics during wound response. (a) Single-cell Ca2+-actin dynamics in wound response. Localized wounding induces a rapid increase in Ca2+ concentration, which triggers the formation of an actin ring around the nucleus, followed by an actin reset to restore normal cytoskeletal organization once healed. (b) Ca2+ and actin dynamics at the multi-cell level in response to wounding. Tissue wounding triggers a Ca2+ increase in surrounding cells, propagating a Ca2+ wave across the tissue. This leads to the coordinated formation of an actin ring at the wound site, promoting collective wound closure. (c) Generalized intracellular Ca2+ dynamics upon wounding across species. Intracellular Ca2+ levels rise through calcium channel influx across the plasma membrane or release from the endoplasmic reticulum (ER) through the IP3 Ca2+ signaling pathway following recognition of a damage/microbe-associated molecular pattern (DAMP/MAMP), chemokines, or other stimulus, activating G protein pathways. Figure created in BioRender.] FIG. 2 (from paper) Calcium signaling and actin dynamics during wound response. (a) Single-cell Ca2+-actin dynamics in wound response. Localized wounding induces a rapid increase in Ca2+ concentration, which triggers the formation of an actin ring around the nucleus, followed by an actin reset to restore normal cytoskeletal organization once healed. (b) Ca2+ and actin dynamics at the multi-cell level in response to wounding. Tissue wounding triggers a Ca2+ increase in surrounding cells, propagating a Ca2+ wave across the tissue. This leads to the coordinated formation of an actin ring at the wound site, promoting collective wound closure. (c) Generalized intracellular Ca2+ dynamics upon wounding across species. Intracellular Ca2+ levels rise through calcium channel influx across the plasma membrane or release from the endoplasmic reticulum (ER) through the IP3 Ca2+ signaling pathway following recognition of a damage/microbe-associated molecular pattern (DAMP/MAMP), chemokines, or other stimulus, activating G protein pathways. Figure created in BioRender. Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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EMBRIO Progress and Continued Funding Efforts
by Brent Thomas Ladd 24 Nov '25

24 Nov '25

[cid:4781ccbc-78b0-42d7-a0b2-446e84fcb0ce] Dear EMBRIO Membership, As an Institute, our collectively achieved progress toward delineating mechanisms of cellular defense and wound repair—properties vital to animal, plant, and human health and biotechnology advancements—is a testament to the power of integrating our expertise. Leveraging this momentum, we are actively assembling teams now to develop new proposals and support co-collaborator proposal writing. Please contact us as soon as possible if you are interested in participating (laddb(a)purdue.edu<mailto:laddb@purdue.edu>, Cc: dumulis(a)purdue.edu<mailto:dumulis@purdue.edu> , jzartman(a)nd.edu<mailto:jzartman@nd.edu> ). We are starting with an NIH RM1 for Multidisciplinary Teams<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgrants.ni…>. For active and potential funding opportunities that align with EMBRIO 2.0 ideas, visit our proposals spreadsheet<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.goog…>. Your willingness to learn, share, and tackle the challenging work of integrating ML/AI, simulation, and life-science research, along with educational research and research into the evolution of interdisciplinary identity and teams, have together made EMBRIO a paradigm for integrative discovery. This integrative effort, since late 2021 involving over a hundred investigators, staff, and trainees, has yielded impactful products and fundamental discoveries (a Summary of Outcomes<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fapp.box.c…> PDF is available on our shared Box account). Perhaps the most important legacy is our trainees. The advancement of sixty-five graduate students and postdocs and sixty undergraduate researchers, at the intersection of predictive computational simulations and biology is directly supporting a growing community of practice, and the long-term growth of the U.S. Bioeconomy. Keep up the transformative research and education to make this fifth year as a Biology Integration Institute our best one yet! 🦃 Enjoy the Thanksgiving Holiday. Your EMBRIO Leadership Team, Leadership Role David Umulis Institute Co-Director Jeremiah Zartman Institute Co-Director Chris Staiger Associate Director Anjali Iyer-Pascuzzi Team Science Lead Alejandra Magana Educational Science Lead Elsje Pienaar Core Modeling Lead Janice Evans Community Advocate Lead Brent Ladd Managing Director

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EMBRIO Weekly Update: Presentation recordings + New Publication in J. Cell Biol.
by Brent Thomas Ladd 20 Nov '25

20 Nov '25

[cid:916c62ac-7c52-4a7f-bf7a-f2e97a6434ee] Dear EMBRIO Members, All-Hands Labs Thanks again to recent presenters this past Monday, Alex Nguyen (Ph.D. Student in the Chan Lab) with Chrissy Boody (Ph.D. Student in the Evans Lab), and Chang Ding (Ph.D. Student in the Deng Lab) presenting their collaborative research: "Measuring cell and tissue-level stiffnesses with atomic force microscopy" in oocytes and zebrafish. On November 3rd we featured the Kim lab with Ph.D. student Jeffery Coulter presenting his collaborations with the Evans Lab and Kinzer-Ursem Lab on "Hybrid models of the mouse egg and dendritic spine", and presented June Kim's work and future directions Modeling the plant cortical cytoskeleton with the Staiger and Iyer-Pascuzzi Labs . You can locate recordings, chat files, and summaries of these talks, and all of the Institute's previous regular seminar sessions going back to 2021 on our shared Box drive under EMBRIO Institute > All-Hands Meeting Recordings: https://app.box.com/s/48uqgrgoy5ygp1tsj2l3lafoy8ubgkop<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fapp.box.c…> (please note these are for use w/in EMBRIO, and not to be distributed w/o permission). ________________________________ New Publications Exciting New Research on Immune Cell Navigation! Led by the Qing Deng Lab, four EMBRIO Institute labs (Deng, Staiger, Jayant, Zhang) have collaborated on research just published online in Journal of Cell Biology. Results point to a critical role for inwardly rectifying potassium channels (Kir) in guiding neutrophils during chemotaxis. Wang, T., D.H. Kim, C. Ding, D. Wang, W. Zhang, M. Silic, X. Cheng, K. Shao, T.H. Ku, C. Zheng, J. Xie, C., Xiao, S., Jayant, K., Yuan, A. Chubykin, C.J. Staiger, G.J. Zhang, and Q. Deng. (2026). Inwardly rectifying potassium channels regulate direction sensing during neutrophil chemotaxis. J. Cell Biol., 225(1): pgs. https://doi.org/10.1083/jcb.202503037<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> 🔍 Key Findings: * Kir7.1 helps maintain resting membrane potential and enables directional sensing in neutrophils. * Disrupting Kir channels impairs the ability of immune cells to orient toward chemo-attractants. * Using voltage indicators and optogenetics, show that membrane potential oscillations are integral to pseudopod selection and migration. 💡 This work introduces membrane potential as a key player in the feedforward mechanisms that steer immune cells through complex tissue environments—linking electrical signaling with GPCR pathways and cytoskeletal dynamics. 📄 Read the full article here: Inwardly rectifying potassium channels promote directional sensing during neutrophil chemotaxis<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Frupress.o…> [cid:41a99081-9b40-4606-8de9-0dbcf0b0fb97] Find our list of published papers and pre-prints on the EMBRIO Institute Google Scholar page<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fscholar.g…>, as well as a full list of scholarly products<https://www.purdue.edu/research/embrio/research/scholarly-products.php> <https://www.purdue.edu/research/embrio/research/scholarly-products.php> at our website. Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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All-Hands Lab Today 3pm Alexandria Nguyen (Chan Lab) "Measuring cell and tissue-level stiffnesses with atomic force microscopy"
by Brent Thomas Ladd 17 Nov '25

17 Nov '25

[cid:b7afd18c-656d-43fd-858f-5e96be6c6b28] Dear EMBRIO Members, Our All-Hands Lab session this afternoon starts at 3pm ET featuring Alexandria Nguyen (Ph.D. Student in the Chan Lab) presenting her talk titled "Measuring cell and tissue-level stiffnesses with atomic force microscopy" . The talk and discussion will feature work in collaboration with the Evans and Deng labs: https://purdue-edu.zoom.us/j/92061591849?pwd=wclgpzLpzWKsZ9M2XYwM7Kbumaabr3…<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue-ed…> See you all this afternoon, Brent Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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EMBRIO Weekly Update
by Brent Thomas Ladd 14 Nov '25

14 Nov '25

[cid:6ad0b25d-55e9-40b2-9ac2-9ce1398ea1e9] Dear EMBRIOphytes, ALL-HANDS LAB Our next All-Hands Lab session will be this coming Monday, Nov. 17th, 3pm with Alexandria Nguyen (Chan Lab) presenting her talk titled "Measuring cell and tissue-level stiffnesses with atomic force microscopy" and will feature work in collaboration with the Evans and Deng labs: https://purdue-edu.zoom.us/j/92061591849?pwd=wclgpzLpzWKsZ9M2XYwM7Kbumaabr3…<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue-ed…> NEW EMBRIO PUBLICATIONS A new paper in PLOS Computational Biology is available: "Surrogate modeling of Cellular-Potts Agent-Based Models as a segmentation task using the U-Net neural network architecture." Congrats to James Glazier and his collaborators on this new work that employs a U-Net convolutional neural network to accelerate simulations of Cellular Potts Models (CPMs): https://doi.org/10.1371/journal.pcbi.1013626<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> 🔬 Key Findings of the Paper: * The authors developed a deep learning surrogate model using a U-Net convolutional neural network to accelerate simulations of Cellular Potts Models (CPMs). * CPMs are widely used to simulate multicellular biological systems, such as vasculogenesis. * The surrogate model predicts future states of CPMs up to 562× faster than traditional simulations, while preserving key emergent behaviors like vessel sprouting, extension, and anastomosis. * This approach enables efficient modeling of complex biological processes over larger spatial and temporal scales. There are several EMBRIO collaborative research papers that will be published in the next month that we'll highlight here in future announcements. As papers are made available live by the jounrnals, they are added to our Google Scholar page<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fscholar.g…>. See you all next Monday afternoon! Brent Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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Reminder: Today All-Hands Lab 3pm EST
by Brent Thomas Ladd 03 Nov '25

03 Nov '25

[cid:24243977-3891-4b4e-97f1-6fce7123867c] Dear EMBRIO Members, Join us this afternoon 3-4pm EST featuring the Kim lab with Ph.D. student Jeffery Coulter presenting on collaborations with the Evans Lab and Kinzer-Ursem Lab in Thrust 1 with modeling Ca2+ and actin in dendritic spines and oocytes! Zoom link: https://purdue-edu.zoom.us/j/92061591849?pwd=wclgpzLpzWKsZ9M2XYwM7Kbumaabr3…<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue-ed…> Coming up on Nov. 17th Alexandria Nguyen (Chan Lab) will present AFM and Imaging collaborations with Evans Lab, Zartman Lab, and Deng Lab. Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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Weekly Update
by Brent Thomas Ladd 29 Oct '25

29 Oct '25

[cid:0c45820d-b7a7-49f6-957a-02a9a3631cc8] Dear EMBRIO Members, This coming Monday 3-4pm ET will feature the Kim lab with Ph.D. student Jeffery Coulter presenting on his collaborations with the Evans Lab and Kinzer-Ursem Lab in Thrust 1 with modeling Ca2+ and actin in dendritic spines and oocytes. Upcoming All-Hands Labs (3PM ET): Date Lead Speaker(s) Topic November 3 Jeffery Coulter (Kim Lab) – collaborations with Evans Lab and Kinzer-Ursem Lab Modeling Ca2+ and Actin in Dendritic Spines and Oocytes November 17 Alex Nguyen (Chan Lab) – collaborations with Evans Lab, Zartman Lab, Deng Lab AFM and Imaging ________________________________ LSFM Workshop Happening This Week EMBRIO teamed up with the Purdue Imaging Facility and Bruker to offer a three-day light-sheet fluorescence microscopy (LSFM) workshop on the Purdue campus this week, with hands-on demonstrations continuing today and tomorrow using the Bruker MuVi SPIM system. Participants are learning fundamental principles, exploring the applications in biological research, while practicing imaging workflows with expert guidance. Thank you to Dr. Weiwei Zhang, and Ph.D. student Nissa Larson for presentations yesterday highlighting how they have each used LSFM in their EMBRIO related research. ________________________________ Awards: 👏 Congratulations to Soumi Mukherjee (Gardner Lab) on her award-winning poster presentation at the recent 150th Anniversary of the Purdue Biological Sciences symposium! Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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End of Week Announcements
by Brent Thomas Ladd 24 Oct '25

24 Oct '25

EMBRIOphytes, 🧬 New Publications Alert! * EMBRIO Members from the Zartman Lab have a newly published paper in Cell Communication and Signaling (first co-authors: Maria F. Unger, Vijay Velagala, Dharsan K. Soundarrajan) "Gαq controls organ size and developmental timing in Drosophila" Summarized findings and link to the full paper is below. 👏 Congrats to the team: Maria F. Unger, Vijay Velagala, Dharsan K. Soundarrajan, David Gazzo, Nilay Kumar, Marycruz Flores Flores, Jinwen Liu, Jun Li & Jeremiah J. Zartman. * Also, you heard it here first: The Calcium Signaling Journal Club's sustained collaborations have produced a newly accepted paper for publication in APL Bioengineering: "Rules of Life at the Interface of Calcium Signaling and Mechanobiology". This significant cross-thrust effort proposes a unifying framework where calcium signaling governs biological organization across scales. 👏 Congratulations to the full team! Once it is publicly available, we'll highlight the paper and the framework introducing three proposed Rules of Life. In the meantime, you can access the preprint version<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.prepr…>. * In case you missed our previous publication highlight, a recent paper elucidating ROS and wound healing led by the Deng Lab collaborating with the Umulis, Chan, and Buganza labs was published in Biophysical Journal: “Reactive Oxygen Species Counteract Zebrafish Wound Contraction and Promote Wound Healing” by C. Ding, L. Li, Y. Wang, H.A.A. Nguyen, D.D. Chan, D.M. Umulis, A.T. Buganza, and Q. Deng. Read the full article here: https://doi.org/10.1016/j.bpj.2025.09.027<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> * A manuscript has been accepted for publication in Journal of Cell Biology resulting from collaborations between the Deng lab and the EMBRIO members of Staiger, Jayant, and Zhang Labs: "Inwardly rectifying potassium channels promote directional sensing during neutrophil chemotaxis". We'll have a highlight out once the paper is publicly available. Publications Highlight: 📄 Gαq controls organ size and developmental timing in Drosophila Unger, M.F., Velagala, V., Soundarrajan, D.K. et al. Gαq controls organ size and developmental timing in Drosophila. Cell Commun Signal 23, 457 (2025). https://doi.org/10.1186/s12964-025-02449-9<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> Using the fruit fly Drosophila melanogaster as a model, the team uncovered how the G protein alpha subunit Gαq regulates epithelial growth and developmental timing. Overexpression of Gαq in wing discs led to reduced wing size and systemic developmental delay—mediated by Ca²⁺-dependent Dilp8 signaling. 🧠 Key findings: •Gαq overexpression decreases cell proliferation and apoptosis •Activates JAK/STAT and stress-response pathways •Triggers secretion of Dilp8, a hormone coordinating growth and timing •Links GPCR signaling to systemic developmental coordination 💡 Why it matters: This work sheds light on how Gαq signaling influences organ size and timing, with implications for understanding diseases like Sturge-Weber syndrome and uveal melanoma, where Gαq mutations are prevalent. 👏 Congrats to the team: Maria F. Unger, Vijay Velagala, Dharsan K. Soundarrajan, David Gazzo, Nilay Kumar, Marycruz Flores Flores, Jinwen Liu, Jun Li & Jeremiah J. Zartman. ________________________________ Upcoming All-Hands Labs (3PM ET): Date Lead Speaker(s) Topic November 3 Jeffery Coulter (Kim Lab) – collaborations with Evans Lab and Kinzer-Ursem Lab Modeling Ca2+ and Actin in Dendritic Spines and Oocytes November 17 Alex Nguyen (Chan Lab) – collaborations with Evans Lab, Zartman Lab, Deng Lab AFM and Imaging Thanks again to Linlin Li (Sr. Research Scientist, Umulis Lab) and Shenyu Lu (Ph.D. student in Joy Wang Lab) for presenting their collaborative findings and future plans last Monday: "AI-POWERED ACCELERATION AND PARAMETER TUNING FOR COMPUTATIONAL MODELING” . The video recording, transcript, and summary notes are available on our shared Box account: https://app.box.com/s/ftdi5zwn7040mhigbn8e5qs5ha8ecgm5<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fapp.box.c…> ________________________________ Awards: 👏 Congratulations to David Gazzo (Zartman Lab) who won a Graduate Student Travel Award from BMES to participate in the Cellular and Molecular Bioengineering (CMBE) conference this coming January. ________________________________ Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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Announcements: Today's All-Hands Topic and Registration for the LSFM Workshop
by Brent Thomas Ladd 20 Oct '25

20 Oct '25

Dear EMBRIO, Plan to join our all-hands lab meeting today at 3pm on AI-POWERED ACCELERATION AND PARAMETER TUNING FOR COMPUTATIONAL MODELING (details and zoom link below). Discover the power of Light-Sheet Fluorescence Microscopy for fast, high-res 3D imaging! Register (free) for the LSFM Workshop<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> (taking place 10/28-29 at Purdue, flyer attached with QR codes and details; basic info below). All-Hands Lab Meeting Today at 3pm ET "AI-POWERED ACCELERATION AND PARAMETER TUNING FOR COMPUTATIONAL MODELING” in a collaboration between the Umulis Lab (Biomedical Engineering) and Wang Lab (Electrical and Computer Engineering). Linlin Li (Sr. Research Scientist, Umulis Lab) will start with the backgrounding concepts and previous achievements, and Shenyu Lu (Ph.D. student in Joy Wang Lab) will present recent progress and findings. Zoom link: https://purdue-edu.zoom.us/j/92061591849?pwd=wclgpzLpzWKsZ9M2XYwM7Kbumaabr3…<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue-ed…> Upcoming All-Hands Labs (3PM ET): Date Lead Speaker(s) Topic November 3 Jeffery Coulter (Kim Lab) – collaborations with Evans Lab and Kinzer-Ursem Lab Modeling Ca2+ and Actin in Dendritic Spines and Oocytes November 17 Alex Nguyen (Chan Lab) – collaborations with Evans Lab, Zartman Lab, Deng Lab AFM and Imaging Light-Sheet Fluorescence Microscopy Workshop: Advanced 3D Imaging for Biological Research Led by Purdue Bindley Bioscience Center (with Dr. Weiwei Zhang), together with Bruker and EMBRIO Institute. This is an excellent opportunity for EMBRIO members to have focused instruction and gain skills in this imaging modality. There are hands-on opportunities to bring your own samples. The workshop will be held October 28-29, 2025, on the Purdue campus, with options for the first half of day one via Zoom. For EMBRIO members at our partner locations interested in attending this training in person, please contact Brent regarding travel (laddb(a)purdue.edu) For questions about the workshop please contact Weiwei (zhan2190(a)purdue.edu ). See the attached flyer and registration page for details. Registration is free: https://purdue.ca1.qualtrics.com/jfe/form/SV_1GrEoUEHdatNBFY<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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Announcements: LSFM Workshop Oct. 28-29, All-Hands Lab Monday Oct. 20
by Brent Thomas Ladd 17 Oct '25

17 Oct '25

[cid:3772eba9-6958-434e-abf6-a74456367cc3] Dear EMBRIOphytes, Two reminders on this beautiful autumn Friday - 1) Register for the LSFM Workshop ASAP, and 2) plan to join our all-hands lab meeting Monday afternoon led by Linlin Li (Umulis Lab) on the use of neural networks in EMBRIO research. Light-Sheet Fluorescence Microscopy Workshop: Advanced 3D Imaging for Biological Research Led by Purdue Bindley Bioscience Center (with Dr. Weiwei Zhang), together with Bruker and EMBRIO Institute. This is an excellent opportunity for EMBRIO members to have focused instruction and gain skills in this imaging modality. There are hands-on opportunities to bring your own samples. The workshop will be held October 28-29, 2025, on the Purdue campus, with options for the first half of day one via Zoom. For EMBRIO members at our partner locations interested in attending this training in person, please contact Brent regarding travel (laddb(a)purdue.edu) For questions about the workshop please contact Weiwei (zhan2190(a)purdue.edu ). See the attached flyer and registration page for details. Registration is free: https://purdue.ca1.qualtrics.com/jfe/form/SV_1GrEoUEHdatNBFY<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> All-Hands Lab Meeting October 20th at 3pm ET Plan to join our All-Hands Lab Meeting Monday, October 20th at 3pm ET, featuring research incorporating neural networks with presentation led by Linlin Li (Umulis Lab) and collaborators. Zoom link: https://purdue-edu.zoom.us/j/92061591849?pwd=wclgpzLpzWKsZ9M2XYwM7Kbumaabr3…<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue-ed…> Thank you to Razeen Shaikh for presenting during our previous session "MIDOE: Maximally-informed Design of Experiment to infer experimentally inaccessible transcription factors dynamics". If you missed or would like to access the associated video and materials (including a summary of the session and Q&A) this is available on our shared Box account under All-Hands Meeting Recordings > 2025, direct link: https://app.box.com/s/nmvz4sy940bvse6m4tdkl94i1eujf11m<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fapp.box.c…> Upcoming All-Hands Labs (3PM ET): Date Lead Speaker(s) Topic October 20 Linlin Li (Umulis Lab) collaborations with Wang Lab and others Neural Networks November 3 Jeffery Coulter (Kim Lab) – collaborations with Evans Lab and Kinzer-Ursem Lab Modeling Ca2+ and Actin in Dendritic Spines and Oocytes November 17 Alex Nguyen (Chan Lab) – collaborations with Evans Lab, Zartman Lab, Deng Lab AFM and Imaging Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

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