December 2025 - Embrio-list

Announcements
by Brent Thomas Ladd 15 Dec '25

15 Dec '25

[cid:e0497c51-4ea6-40f5-aaf6-324477f23182] Dear EMBRIO Members, Best wishes for all involved in final exams and courses as your respective Fall semesters wrap up! Congrats to the team of EMBRIO members that organized, chaired, and presented an Integrative Cell and Developmental Systems Biology Mini-Symposium last week at CellBio 2025 in Philadelphia (see below). Don't forget to share your input, preferences, and availability for our annual summer events - quick online survey<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> <https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> (details below). A reminder that we won't meet this afternoon for All-Hands during finals (we have a focused proposal discussion happening this afternoon. Please use this Google spreadsheet<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.goog…> for coordinating interest and activity around a number of funding opportunities associated with EMBRIO and/or contact Brent, laddb(a)purdue.edu .) We are balancing the spring semester All-Hands Lab sessions between research thrust updates and working proposal team sessions. Separate calendar invites for these sessions with dedicated Zoom links have been emailed out to the membership (more on this below). Purdue EMBRIO Faculty: SUMMER UNDERGRADUATE RESEARCH FELLOWSHIP (SURF) program is accepting faculty project postings details below. ________________________________ EMBRIO Mini-Symposium at American Society for Cell Biology (ASCB)<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…> Cell Bio 25 a Success The EMBRIO Mini-Symposium at Cell Bio25 was a great success with around 100 attendees and an eight member EMBRIO team involved. Thanks to the entire team for organizing, chairing, and presenting your cutting-edge research to the ASCB community! (and the team had the added bonus of touring the Mullins Lab at UPenn 🙂 ). [cid:2c72b96d-0b7b-4a5a-ba47-a4fbd9fb1778] EMBRIO Team at Cell Bio 25 (L-R): Greg Reeves, Mary Mullins, Bakary Samasa, Nissa Larson, Chang Ding, Linlin Li, Qing Deng, Jeremy Zartman EMBRIO Integrative Cell and Developmental Systems Biology Mini-Symposium @CellBio 2025 – An ASCB | EMBO Meeting December 6–10, 2025 | Philadelphia, PA Mary Mullins and Qing Deng<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…>, Symposium Chairs •Aggregation and DNA binding of Dorsal/NF-κB in early embryos — Gregory Reeves<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…> •Signal processing and robustness of the BMP network during zebrafish embryogenesis — Nissa Larson<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…> •Reactive Oxygen Species Counteract Zebrafish Wound Contraction and Promote Wound Healing — Chang Ding<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…> •Reverse engineering organ size and shape control through the integration of quantitative experiments and multi-scale computational modeling — Jeremiah Zartman<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…> •Investigating cytoskeletal and cellular dynamics during morphogenesis in the zebrafish — Bakary Samasa<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…> •AI-powered acceleration and parameter tuning for computational modeling — LINLIN LI<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…> ________________________________ Reminder: We Need Your Input: Dates, Plans, Topics for EMBRIO 2026 Summer School & Joint Symposium Use this brief online poll to capture your availability, plans and preferences, as well as input for training topics: https://purdue.ca1.qualtrics.com/jfe/form/SV_bkIw8bHUboD9Qwu<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> A joint EMBRIO BII NSF - Notre Dame (RECODE NSF) one day symposium and poster session has been proposed focused on "Advancing Morphogenetic Biosciences and Bioengineering”, coupled with a multi-day training at the intersection of AI-levered computational modeling, quantitative biology, and bioengineering (e.g. https://sai.nd.edu/<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fsai.nd.ed…>). The Symposium will feature EMBRIO speakers, along with speakers from Notre Dame, and invited Key Notes. Proposed to be hosted at our partners at University of Notre Dame, South Bend, Indiana, EMBRIO will organize and help provide transportation and lodging for Purdue members to attend, as well as reimburse travel for EMBRIO trainees from our partner institutions to participate. ________________________________ Reminder: Spring Semester EMBRIO All-Hands Schedule: Mondays 3-4pm Biweekly We will continue with Mondays bi-weekly 3-4pm ET (calendar invites will be sent out soon. The intention this spring semester is to have one session a month dedicated to research thrust updates and one session a month dedicated to working proposal team(s) collaborating on new funding proposals that leverage EMBRIO (trainees are welcome to attend/participate in these sessions). * Dates for Research Thrust Updates will be Jan 26, Feb 23, Mar 23, April 20, May18. * Brent will be in touch with Thrust leaders and trainees for scheduling these times. * Dates for Working Proposal Teams will be Jan 12, Feb 9, Mar 9, April 6, May 4. * Working teams will receive additional notifications and materials. * Please use this Google spreadsheet<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.goog…> for coordinating interest and activity around a number of funding opportunities associated with EMBRIO. * Proposal teams will have additional meeting times, though these dedicated times in the spring schedule can help foster coordination and ties between collaborative proposals and activity in this space that is leveraging EMBRIO research. ________________________________ Purdue EMBRIO Faculty: SUMMER UNDERGRADUATE RESEARCH FELLOWSHIP (SURF)- Accepting project postings! Purdue’s SURF program<https://maillinks.purdue.edu/t/48863454/1704294751/89814806/0/1007625/?x=70…> invites faculty to mentor outstanding undergraduates in an 11-week paid summer research experience at the West Lafayette or Indianapolis campuses. By mentoring a SURF student, faculty help shape future researchers while contributing to student growth and professional development. EMBRIO covers the PI amount of the internship cost and a housing stipend for non-Purdue students (your department and college, depending on the agreement, kick in the other 2/3 of the cost) Please let Brent know if you submit an EMBRIO project and indicate under the Centers/Institutes checkboxes that the project is an EMBRIO project. Submit project postings here<https://maillinks.purdue.edu/t/48863454/1704294751/107443535/0/1007625/?x=a…> ________________________________ Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

1 0

End of Week Reminders
by Brent Thomas Ladd 12 Dec '25

12 Dec '25

Dear EMBRIOnic People, A few reminders prior to the end of the semester: Please share your input, preferences, and availability for our annual summer events - quick online survey<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> <https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> (details below). We won't meet on Monday for All-Hands during finals week. We are balancing the spring semester All-Hands Lab sessions between research thrust updates and working proposal team sessions Please use this Google spreadsheet<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.goog…> for coordinating interest and activity around a number of funding opportunities associated with EMBRIO). Separate calendar invites for these sessions with dedicated Zoom links have been emailed out to the membership (more on this below). We Need Your Input: Dates, Plans, Topics for EMBRIO 2026 Summer School & Joint Symposium Use this brief online poll to capture your availability, plans and preferences, as well as input for training topics: https://purdue.ca1.qualtrics.com/jfe/form/SV_bkIw8bHUboD9Qwu<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> A joint EMBRIO BII NSF - Notre Dame (RECODE NSF) one day symposium and poster session has been proposed focused on "Advancing Morphogenetic Biosciences and Bioengineering”, coupled with a multi-day training at the intersection of AI-levered computational modeling, quantitative biology, and bioengineering (e.g. https://sai.nd.edu/<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fsai.nd.ed…>). The Symposium will feature EMBRIO speakers, along with speakers from Notre Dame, and invited Key Notes. Proposed to be hosted at our partners at University of Notre Dame, South Bend, Indiana, EMBRIO will organize and help provide transportation and lodging for Purdue members to attend, as well as reimburse travel for EMBRIO trainees from our partner institutions to participate. Spring Semester EMBRIO All-Hands Schedule: Mondays 3-4pm Biweekly We will continue with Mondays bi-weekly 3-4pm ET (calendar invites will be sent out soon. The intention this spring semester is to have one session a month dedicated to research thrust updates and one session a month dedicated to working proposal team(s) collaborating on new funding proposals that leverage EMBRIO (trainees are welcome to attend/participate in these sessions). * Dates for Research Thrust Updates will be Jan 26, Feb 23, Mar 23, April 20, May18. * Brent will be in touch with Thrust leaders and trainees for scheduling these times. * Dates for Working Proposal Teams will be Jan 12, Feb 9, Mar 9, April 6, May 4. * Working teams will receive additional notifications and materials. * Please use this Google spreadsheet<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.goog…> for coordinating interest and activity around a number of funding opportunities associated with EMBRIO. * Proposal teams will have additional meeting times, though these dedicated times in the spring schedule can help foster coordination and ties between collaborative proposals and activity in this space that is leveraging EMBRIO research. Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

1 0

Announcements: Input Poll, Spring Semester schedule
by Brent Thomas Ladd 08 Dec '25

08 Dec '25

[cid:1f1e373f-23ea-4d8c-9acf-c49a4e80201f] Dear EMBRIO Members, We Need Your Input: Dates, Plans, Topics for EMBRIO 2026 Summer School & Joint Symposium Use this brief online poll to capture your availability, plans and preferences, as well as input for training topics: https://purdue.ca1.qualtrics.com/jfe/form/SV_bkIw8bHUboD9Qwu<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> A joint EMBRIO BII NSF - Notre Dame (RECODE NSF) one day symposium and poster session has been proposed focused on "Advancing Morphogenetic Biosciences and Bioengineering”, coupled with a multi-day training at the intersection of AI-levered computational modeling, quantitative biology, and bioengineering (e.g. https://sai.nd.edu/<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fsai.nd.ed…>). The Symposium will feature EMBRIO speakers, along with speakers from Notre Dame, and invited Key Notes. Proposed to be hosted at our partners at University of Notre Dame, South Bend, Indiana, EMBRIO will organize and help provide transportation and lodging for Purdue members to attend, as well as reimburse travel for EMBRIO trainees from our partner institutions to participate. Spring Semester EMBRIO All-Hands Schedule: Mondays 3-4pm Biweekly We will continue with Mondays bi-weekly 3-4pm ET (calendar invites will be sent out soon. The intention this spring semester is to have one session a month dedicated to research thrust updates and one session a month dedicated to working proposal team(s) collaborating on new funding proposals that leverage EMBRIO (trainees are welcome to attend/participate in these sessions). * Dates for Research Thrust Updates will be Jan 26, Feb 23, Mar 23, April 20, May18. * Brent will be in touch with Thrust leaders and trainees for scheduling these times. * Dates for Working Proposal Teams will be Jan 12, Feb 9, Mar 9, April 6, May 4. * Working teams will receive additional notifications and materials. * Please use this Google spreadsheet<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdocs.goog…> for coordinating interest and activity around a number of funding opportunities associated with EMBRIO. * Proposal teams will have additional meeting times, though these dedicated times in the spring schedule can help foster coordination and ties between collaborative proposals and activity in this space that is leveraging EMBRIO research. Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

1 0

Weekly Update: Science Signaling Paper Makes the Cover and the News; Input Poll for Summer Events; EMBRIO Mini-Symposium at ACSB Cell Bio
by Brent Thomas Ladd 03 Dec '25

03 Dec '25

[cid:4d7c7446-db9a-462a-8270-23f2ccfac277] Dear EMBRIOphytes, This week, we highlight a new paper in Science Signaling born from collaborations between the Staiger, Pienaar, Umulis, and Iyer-Pascuzzi labs, and well wishes to the EMBRIO team presenting a mini-symposium at the ASCB Cell Bio 2025 conference starting this weekend. Please take a few minutes this week to provide your input and preferences<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> for a joint EMBRIO - Notre Dame NSF RECODE Symposium and Summer School next year (more details below and at the online poll). ________________________________ 🚨 New Publication Alert 🚨 [cid:d4b1bb23-d5f2-49ed-b23a-70570c1a89b6] EMBRIO’s interdisciplinary team investigating plant defense has an exciting paper out in Science Signaling (story featured in Purdue News<https://ag.purdue.edu/news/2025/12/plant-first-responder-cells-warn-neighbo…> and made the cover 🎉). “Local traveling waves of cytosolic Ca2+ elicited by defense signals or wounding are propagated by distinct mechanisms in Arabidopsis”. The paper unpacks discoveries advancing the field, that distinct patterns of Ca²⁺ waves in plants may contribute to stimulus-specific responses to mechanical vs. chemical perturbation, as well as single-cell vs. multicellular level responses. Mathematical modeling recapitulated the features demonstrated in the experimental studies. Read the paper<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> Zhang, W., Kumar, N., Helwig, J., Hoerter, A., Iyer-Pascuzzi, A.S., Umulis, D., Pienaar, E., and Staiger, C.J. (2025). Local traveling waves of cytosolic calcium elicited by defense signals or wounding are propagated by distinct mechanisms. Sci. Signal.18, 915. DOI: 10.1126/scisignal.adw2270<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> Huge thanks to all co-authors, collaborators, and funding teams who helped bring this to fruition: Weiwei Zhang<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…>, Nilay Kumar<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…>, Jessica Helwig<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…>, Alexis Hoerter<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…>, Anjali Iyer-Pascuzzi<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…>, David Umulis<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…>, Elsje Pienaar<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…>, Chris Staiger<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.linke…> [cid:0a5603c0-31a2-4a5a-aabe-83fd9bd1b5a6] Caption: Purdue University’s Weiwei Zhang, senior research scientist in botany and plant pathology and a member of the Emergent Mechanisms in Biology of Robustness Integration and Organization (EMBRIO) Institute, prepares a sample for imaging at a confocal microscope. Researchers at Purdue found that a subset of epidermal cells in plant leaves serves as early responders to chemical cues from bacterial pathogens and communicate this information to neighbors through a local traveling wave of calcium ions. The properties of this local wave suggest that distinct mechanisms are used by plants to communicate specific types of pathogen attack. 🔬 Key Insights •Rather than a uniform “on/off” Ca²⁺ spike, spatially and temporally structured waves were observed — a sign that cells encode more information in Ca²⁺ dynamics than previously thought. •These Ca²⁺ waves are tied to activation of downstream signaling pathways that modulate immune response, offering a refined view of how innate immunity may be fine-tuned at the single-cell level. •The new findings provide a framework for rethinking how immune cells interpret and respond to threats — not simply by the magnitude of Ca²⁺ increase, but by the pattern of Ca²⁺ dynamics. 🌍 Why It Matters This work expands the conceptual underpinnings of immune signaling in plants by showing that spatiotemporal patterns of Ca²⁺ flux — not just overall levels — carry important functional information, we open the door to new ways of understanding immune regulation, and potentially, new therapeutic approaches that exploit Ca²⁺ dynamics. ________________________________ We Need Your Input: Dates, Plans, Topics for EMBRIO 2026 Summer School & Joint Symposium A joint EMBRIO BII NSF - Notre Dame (RECODE NSF) one day symposium and poster session has been proposed focused on "Advancing Morphogenetic Biosciences and Bioengineering”, coupled with a multi-day training at the intersection of AI-levered computational modeling, quantitative biology, and bioengineering (e.g. https://sai.nd.edu/<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fsai.nd.ed…>). The Symposium will feature EMBRIO speakers, along with speakers from Notre Dame, and invited Key Notes. Proposed to be hosted at our partners at University of Notre Dame, South Bend, Indiana, EMBRIO will organize and help provide transportation and lodging for Purdue members to attend, as well as reimburse travel for EMBRIO trainees from our partner institutions to participate. Please use this brief online poll to capture your availability, plans and preferences, as well as input for training topics: https://purdue.ca1.qualtrics.com/jfe/form/SV_bkIw8bHUboD9Qwu<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> ________________________________ EMBRIO Mini-Symposium: Integrative Cell and Developmental Systems Biology - December 6-10 ASCB CellBio 2025<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.ascb.…> EMBRIO Members have organized a mini-symposium at this year's ASCB Cell Bio Conference. This is the list of presenters and topics being presented: • Aggregation and DNA binding of Dorsal/NF-κB in early embryos - Gregory Reeves • Signal processing and robustness of the BMP network during zebrafish embryogenesis - Nissa Larson • Reactive Oxygen Species Counteract Zebrafish Wound Contraction and Promote Wound Healing - Chang Ding • Reverse engineering organ size and shape control through the integration of quantitative experiments and multi-scale computational modeling - Jeremiah Zartman • Investigating cytoskeletal and cellular dynamics during morphogenesis in the zebrafish- Bakary Samasa • AI-powered Acceleration and Parameter Tuning for Computational Modeling - Linlin Li Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

1 0

Announcements: Data Mngt session today; Poll for Summer Events; New Publication from Ca++ Journal Club
by Brent Thomas Ladd 01 Dec '25

01 Dec '25

[cid:d1a9c962-22f6-4103-9fcc-65a1f5f77d7a] Dear EMBRIOphytes, I hope everyone was able to enjoy the holiday break. I had the pleasure of taking my sister and her family (who reside in Florida) to the frigid ❄️ Friday night Purdue vs. Indiana football game... to ensure no one would lose a toe or finger we vacated ⛷ after the half-time show ⛄ . Announcements include a reminder to invited labs to attend today's All-Hands data management session, a poll for your input on summer events, and a highlight of the awesome review paper in APL Bioengineering co-authored by members of the Calcium Signaling Journal Club: Today 3pm Data Management Session for Invited Labs Today's EMBRIO All-Hands Lab is dedicated to meeting with specific invited computational and experimental labs on data management tasks and needs. All are welcome, though if you received an email from me last week about attending this session, please do tune in - those who have a time conflict we can get with you another time before the semester ends. Computational labs will likely take 15 minutes or less once we cover GitHub and PURR, and the few experimental labs invited we will use the balance of time to address any imaging data tasks and needs going forward. We Need Your Input: Dates, Plans, Topics for EMBRIO 2026 Summer School & Joint Symposium A joint EMBRIO BII NSF - Notre Dame (RECODE NSF) one day symposium and poster session has been proposed focused on "Advancing Morphogenetic Biosciences and Bioengineering”, coupled with a multi-day training at the intersection of AI-levered computational modeling, quantitative biology, and bioengineering (e.g. https://sai.nd.edu/<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fsai.nd.ed…>). The Symposium will feature EMBRIO speakers, along with speakers from Notre Dame, and invited Key Notes. Proposed to be hosted at our partners at University of Notre Dame, South Bend, Indiana, EMBRIO will organize and help provide transportation and lodging for Purdue members to attend, as well as reimburse travel for EMBRIO trainees from our partner institutions to participate. Please use this brief online poll to capture your availability, plans and preferences, as well as ideas for training topics: https://purdue.ca1.qualtrics.com/jfe/form/SV_bkIw8bHUboD9Qwu<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue.ca…> New EMBRIO Paper in APL Bioengineering! This integrative review exploring the “Rules of Life” at the interface of Calcium Signaling and Mechanobiology involved nine EMBRIO labs spanning all of our biological thrust areas and the Core Thrust! This work focuses on how Ca2+ regulates and is regulated by cytoskeletal dynamics, with a particular emphasis on computational modeling for predictive simulations. In exploring a Rules of Life (RoL) perspective, the team defined and reviewed the multi-scalar principles governing the relationships between calcium signaling and cellular mechanics and organization: * RoL 1: Ca2+ dynamics facilitate cytoskeletal reorganization following stress and damage. * RoL 2: Ca2+ regulates actin dynamics to control synapse processes supporting both synapse formation and exocytosis. * RoL 3: Reciprocal coupling of spatiotemporal Ca2+ signaling and cellular dynamics defines distinct cellular roles in emergent multicellular behavior. 🔗 Link to Paper: https://doi.org/10.1063/5.0284022<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> Congrats to the entire team for this major accomplishment! Linlin Li and David Gazzo contributed equally to this work as co-first authors, while Shams Mowafak Saad, Nissa Larson, Eugene Kim, and Nilay Kumar contributed equally as co-second authors. Jeremiah Zartman, as the senior author, organized and supervised the Calcium Signaling Journal Club that led to this exciting publication. Li, L., Gazzo, D., Saad, S., Larson, N., Kim, E., Kumar, N., Mim, M., Krishnan, M., Speybroeck, B., Ding, C., Mullins, M., Pascuzzi, A., Deng, Q., Pienaar, E., Evans, J., Umulis, D., Zartman, J. Rules of Life at the Interface of Calcium Signaling and Mechanobiology. Open Access November 26, 2025. APL Bioengineering. https://doi.org/10.1063/5.0284022<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2…> [Calcium signaling and actin dynamics during wound response. (a) Single-cell Ca2+-actin dynamics in wound response. Localized wounding induces a rapid increase in Ca2+ concentration, which triggers the formation of an actin ring around the nucleus, followed by an actin reset to restore normal cytoskeletal organization once healed. (b) Ca2+ and actin dynamics at the multi-cell level in response to wounding. Tissue wounding triggers a Ca2+ increase in surrounding cells, propagating a Ca2+ wave across the tissue. This leads to the coordinated formation of an actin ring at the wound site, promoting collective wound closure. (c) Generalized intracellular Ca2+ dynamics upon wounding across species. Intracellular Ca2+ levels rise through calcium channel influx across the plasma membrane or release from the endoplasmic reticulum (ER) through the IP3 Ca2+ signaling pathway following recognition of a damage/microbe-associated molecular pattern (DAMP/MAMP), chemokines, or other stimulus, activating G protein pathways. Figure created in BioRender.] FIG. 2 (from paper) Calcium signaling and actin dynamics during wound response. (a) Single-cell Ca2+-actin dynamics in wound response. Localized wounding induces a rapid increase in Ca2+ concentration, which triggers the formation of an actin ring around the nucleus, followed by an actin reset to restore normal cytoskeletal organization once healed. (b) Ca2+ and actin dynamics at the multi-cell level in response to wounding. Tissue wounding triggers a Ca2+ increase in surrounding cells, propagating a Ca2+ wave across the tissue. This leads to the coordinated formation of an actin ring at the wound site, promoting collective wound closure. (c) Generalized intracellular Ca2+ dynamics upon wounding across species. Intracellular Ca2+ levels rise through calcium channel influx across the plasma membrane or release from the endoplasmic reticulum (ER) through the IP3 Ca2+ signaling pathway following recognition of a damage/microbe-associated molecular pattern (DAMP/MAMP), chemokines, or other stimulus, activating G protein pathways. Figure created in BioRender. Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb(a)purdue.edu

1 0