[cid:image003.jpg@01D96C56.2702C320] BME MONDAY ROUNDTABLE APRIL 10, 2023 DEPUTY HEAD’S NOTE * Time flies. We are coming to the last month of the semester. This tends to be one of the most stressful times of the year. We want to be cognizant of faculty, staff and students’ well-being. Darshini gave an excellent presentation on student well-being in last Friday’s faculty meeting. We got good support among the faculty. I further emphasized the correlation between students’ well-being and the well-being of faculty and staff. Research has shown student success and well-being is influenced largely by their faculty members, whose ability to inspire and lead depends on their own well-being. In addition, as a course instructor, there are ways to help alleviate students’ stress, e.g., low-state assessment, proactive assignment due date scheduling, etc. * Estelle Park has accepted our offer to join our faculty. She will formerly join us on 1/1/2024 as a tenure-track assistant professor. She will bring to the department great expertise in microfluidics, instrumentation, and cell engineering technology. She will have her second visit to West Lafayette from 5/17 – 5/19. * I want to use this opportunity to congratulate Dr. Brad Duerstock for being inducted to the 2023 class of the College of Fellows by the American Institute for Medical and Biological Engineering (AIMBE). More information is available from: https://engineering.purdue.edu/BME/AboutUs/News/2023/23-Duerstock-AIMBE. * The course info form is due this Friday (4/14). Please send it to Cindy. Ideally, you can also send your syllabus, even a draft, to her. * GIK-Gift-in-kind -- Please let Brian Knoy know if you have received any donated equipment, supplies, services, etc. over the last two semesters. Brian can assist on how to document these. * Purdue Day of Giving on April 26th -- Free doughnuts in the atrium for the annual online-based fundraising event. For 24 hours, students, alumni, faculty and staff, parents, retirees, and friends are encouraged to help power Purdue’s next giant leap. * Our advisory board meeting in fall 2023 will be on Friday, September 22nd. Celebrating our current and former students’ achievements from March 2023. Also a big round of applause to the faculty who have advised them and are advising them. * Undergraduate student Niharika Narra (BME ’25) has been accepted to the highly competitive Stanford Cardiovascular Institute Summer Research Program. She is a student researcher in Taimoor Qazi’s lab. * 2023 NSF Graduate Research Fellowship Program (GRFP) awardees: * Mikayla Roach (PhD student in Deva Chan’s Lab) * Josh Kosnoff (BSBME ’22 – now a PhD student at Carnegie Mellon) * Undergraduate student Carl Russell (BSBME ’23, and also continuing at Purdue in the MD/PhD program) received the Charles O. McGaughey Leadership Award from the Office of the Vice Provost for Student Life. He will be recognized at a ceremony on April 16. * Academic All-Big Ten Honorees (3.0+ cumulative GPA) for winter sports: * Charlie King<https://purduesports.com/sports/mens-swimming-and-diving/roster/charlie-king/11866> (swimming and diving) * Maggie Love<https://purduesports.com/sports/womens-swimming-and-diving/roster/maggie-love/11907> (swimming and diving) * Frederick (Freddy) Damen (BME PhD ’21; worked in CVIRL with Craig Goergen as grad research assistant) is a 4th year medical student in IU MSTP and was just matched with the pediatrics physician-scientist program at Stanford. * David Sohutskay (BME PhD ’21; worked with Sherry Harbin and Adrian Buganza Tepole) is also an IU MSTP student just matched with the Mayo Clinic in neurology. Important MJIS Alert Starting Monday, May 22nd, Martin Jischke Drive will be CLOSED to all traffic. The university will be performing infrastructure repairs in the area. The street will be closed to traffic for most, if not all of the summer. When the street reopens, bike lanes will replace the on-street parking, although we do not currently have details/confirmation on the entire process yet. Stay tuned for additional details. UPCOMING IMPORTANT DATES Week of April 10 Tues., April 11: BME-IBSC PhD Defense Announcement for Antonia Šušnjar (J. Rispoli and T. Talavage, Co-Chairs), 1:00 p.m., Purdue Graduate Student Center, Room 105 and via Zoom. Title of the Thesis Research: Clinical Applications of Magnetic Resonance Spectroscopy Tues., Apr. 11: Shah Family Global Innovation Lab 2023 I2D Expo, Student Poster Session, Dudley Atrium, 11:30 a.m. - 1:30 p.m., Seed Grant Showcase, WSLR 116, 2:30-4:30 p.m., Keynote and Awards, ME 1130, 5:30-7:00 p.m. Go here to register for all events and for additional information: https://engineering.purdue.edu/GEP/ShahLab/Expo2023/Expo_2023 Wed., April 12: BME Distinguished Seminar Series, 9:30 a.m. MJIS 1001 and via Zoom. Chien-Chi Lin, Thomas J. Linnemeier Guidant Foundation Endowed Chair and Professor of Biomedical Engineering, Indiana University-Purdue University Indianapolis will present “Click hydrogels for pancreatic tissue engineering.” Wed., April 12: BME-IBSC PhD Defense Announcement for Jennifer L. Anderson (C. Goergen, advisor), 12:30 p.m., MJIS 2001 and via Zoom. Title: The Role of Renal Compartment Syndrome in renal injury during pregnancy Wed., April 12: Diabetes Research Virtual Seminar Series, 2:00 p.m., via Zoom. Leonardo Trasande, MD, MPP, Jim G. Hendrick, MD Professor of Pediatrics, Director, NYU Center for the Investigation of Environmental Hazards, NYU Grossman School of Medicine, will present “Endocrine Disrupting Chemicals as Metabolic Risks and What We Can Do About It. Zoom link: https://zoom.us/j/91323894836 Thurs., April 13: BME PhD Defense Announcement for Ángel G. Enríquez Mujica (H. Lee, advisor), 9:00 a.m., MJIS 2001 and via Zoom. Title: Magnetic actuators for applications in biomedical devices and a novel thrombectomy catheter. Thurs., April 13: College of Veterinary Medicine Special Seminar, 12:30 p.m., LYNN 1136 and via Zoom. Dr. Aradhya Gourapura, Professor and Interim Director, Center for Food Animal Health, Department of Animal Sciences, Ohio State University will present “Nanoparticle Nature Dictates the Type of Immune Response and Efficacy of Nanovaccines: Studies in a Pig Model.” Zoom link: https://purdue-edu.zoom.us/j/6308671867 Upcoming important deadlines and info. Mon., April 17: BME PhD Preliminary Exam Announcement for Kentaro Umemori (D. Little, advisor). Everyone is invited to attend the public presentation beginning at 9:30 AM EST in MJIS 2001. Research Title: The Impact of Polymer on 3D Meltblown Scaffolds and the Epigenome of Adipose-Derived Progenitors Tues., April 18: BME PhD Defense Announcement for Peiyi Zhang (Fang Huang, advisor), 10:00 a.m., MJIS 2001 and via Zoom. Title of Thesis: Single molecule analysis and wavefront control driven by deep learning. Everyone is invited to attend the public presentation beginning at 10:00 a.m. Tues., April 18: BME Master's Defense Announcement for Pankti R. Thakkar (J. Linnes, advisor), 12:00 p.m., BRK 2001 and via Zoom. Thesis Title: Characterization and Development of an Enzymatically Signal Enhanced Lateral Flow Assay Test for HIV using p24 Antigen. Everyone is invited to attend the public presentation beginning at 12:00 pm. Wed., April 19: Seminar for Neurotrauma and Diseases, 4:00 p.m., DLR 131 and via Zoom. Dr. Kinam Park, Showalter Distinguished Professor of Biomedical Engineering at Purdue will present Drug Delivery Systems: The Revolution-Evolution Cycle. Zoom link: https://bit.ly/3HsTf5R Seminars are sponsored by Plexon and the Center for Paralysis Research at Purdue. Thurs., April 20: BME Master's Defense Announcement for Riley J. Brown (J. Linnes, advisor). Everyone is invited to attend the public presentation beginning at 12:00 pm in BRK 1001 and via Zoom. Title: Characterization and Implementation of Screen Printed, Flexible PTC Heaters for Portable Diagnostic Testing Fri., April 21: Upcoming Indiana Life Sciences Collaboration Conference: Digital Health – The “Buzz” and the Reality,” 8:00 a.m. – 5:00 p.m., Taft-Regions Tower, Indianapolis. The FDA defines digital health as “mobile health (mHealth), health information technology (IT), wearable devices, telehealth and telemedicine, and their relationship to personalized medicine”. The pandemic accelerated its adoption across healthcare. This conference will take a look at the progress that has actually been made, further opportunities and issues still needing to be ironed out so digital health can reach its full potential. For more information and to register, go to: https://kelley.iu.edu/faculty-research/centers-institutes/business-of-life-s... Tues., Apr. 25-Thurs., Apr. 27: Artificial Intelligence in Healthcare Innovation: Moving from Reactive to Proactive Conference hosted by the Regenstrief Institute and Indiana University School of Medicine, Department of Biostatistics & Health Data Science, April 25-27. Registration is free, and available at regenstrief.org/ai-conference-2023 Fri., May 12-Sat., May 13: Inaugural Krannert Cardiovascular Research Center Biennial, sponsored by the Indiana University School of Medicine at the JW Marriott and Eiteljorg Museum, Indianapolis, IN. Krannert is conducting a nationally recognized CME event May 12-13 (Fri-Sat) on Myocardial Infarction & Reperfusion Injury. We cordially invite you to join us if possible. We have a strong line up of speakers on the topic who are renowned for their decades of contributions to the field. We are hoping for an event that would capture almost 50 years of activity in the field of ischemia/reperfusion and place it in a context to chart the course forward with the goal of improving care for heart attack patients. This is a great opportunity to meet thought leaders, engage in transformative science, and hopefully be part of a journey to advance CV research. For Program Agenda & Registration, please follow the link: https://medicine.iu.edu/institutes/cardiovascular/research/biennial Fri., May 12: Diabetes Data and Hypothesis Hub (D2H2) Seminar, 12:00 p.m., via Zoom. Dr. Avi Ma’ayan will present "The Diabetes Data and Hypothesis Hub (D2H2) and the Playbook Partnership Workflow Builder (PPWB): Bioinformatics Tools for Hypothesis Generation via Data Integration.” Register here: https://scgcorp.zoom.us/webinar/register/WN_YgWw6WDTRX6TfaugZ3OBXg Thurs., May 25: Application Deadline for Trailblazers in Engineering workshop, to be held on the Purdue campus July 25-26. Trailblazers in Engineering (TBE) is a multi-day workshop focused on preparing future outstanding engineering scholars for future engineering faculty careers who are also committed to increase the success of underrepresented communities of engineers. Trailblazers in Engineering Fellows are selected not only for their outstanding scholarly achievements but also for their potential impact in expanding representation and diversity in engineering. See attached flyer for application instructions. Committee Updates Academic Programs Update REMINDER to Fall teaching faculty: The teaching schedule for Fall has been published and for those offering classes, you should be able to view your course within MyPurdue or the online Schedule of Classes. Fall deadlines are already approaching. Therefore, please complete the attached Course Info Form about the need for any textbooks OR supplemental materials, AND the need for any exams (Evening or Final Exams) for your course. Please return the attached form to Cindy Holderbaum as soon as you know that information or no later than Friday, 4/14. Also send Cindy the syllabus for your course. We will accept drafts at first, but please remember to send the final one whenever it is ready. The basic requirements for a syllabus, as mandated by the provost, can be found here: https://www.purdue.edu/innovativelearning/developing-courses/syllabus-templa... Cindy will upload syllabi into the Registrar’s Course Insights website on your behalf. Graduate Office Update Dear BME Faculty, Weldon BME PhD students performing research in one of these areas - cardiovascular devices, implantable biomaterials design, drug-delivery systems, or biochemical sensors – are eligible for nomination for the Ronald W. Dollens Scholarship. For the nomination, we will need from you a short description (less than 250 words) of an outstanding, current graduate student in your lab who is excelling in research performance and service to the department. In your description, please mention/include the following: * Student’s year in the PhD program (1st year, 2nd year, etc.) * Brief description of outstanding research performance * Brief description of service to the department * Mention of recent awards, presentations made at conferences, papers authored, etc., that you think help describe the student’s contributions * Student’s CV Please send the nomination to tsiemers@purdue.edu<mailto:tsiemers@purdue.edu> by Sunday, May 21st. The Ronald W. Dollens Scholarships in Life Sciences will be awarded based on academic merit to two deserving graduate students in the Weldon School of Biomedical Engineering who meet the criteria and are selected. The scholarship award is $5,000 with half being awarded in the Fall 2023 semester and half in the Spring 2024 semester. If you have any questions, please don’t hesitate to reach out. Spring 2023 BME Seminar Schedule (Wednesdays, 9:30 a.m. in MJIS 1001). Full schedule below. New Course for Fall 2023: BME 69500 Pediatric Medical Devices (CRN 14011). Details below. BMEGSA General Body Meetings – All BME graduate students are welcome, and encouraged to attend! Upcoming meetings: Thursday, April 20th 3:30PM-4:30PM All meetings are held in MJIS 2001 in person only. Graduate Admissions Update Fall 2022 (Fall 2023 Admit Term) BME Graduate Admissions DateDATE Activity April 15 Graduate applicant acceptance deadline Research Update To safeguard our valuable work and bolster Weldon's standing, it is important that we exercise caution with predatory journals and “hidden” predatory journals. We are collaborating with the Purdue library to organize a session. Stay tuned. https://www.science.org/content/article/fast-growing-open-access-journals-st... https://retractionwatch.com/2023/03/21/nearly-20-hindawi-journals-delisted-f... Participants needed for a Motor Function Study, “Development of a Diagnostic System for Assessment of Motor Impairment,” IRB# IRB-2022-1299. See below for details. Resources and further information BME-IBSC PhD Defense Announcement for Antonia Šušnjar (J. Rispoli and T. Talavage, Co-Chairs), Tues., April 11, 1:00 p.m., Purdue Graduate Student Center, Room 105 and via Zoom. Title of the Thesis Research: Clinical Applications of Magnetic Resonance Spectroscopy Thesis Committee members: Dr. Joseph Rispoli, Major Professor, Co-Chair; Dr. Thomas Talavage, Co-Chair; Dr. Ulrike Dydak; Dr. Uzay Emir Abstract: Magnetic resonance spectroscopy (MRS) is a non-invasive imaging technique that provides unique information about the biochemical composition of the human body. By excluding the overwhelming signals from water and fat, clinically relevant biomarkers such as lactate, N-acetyl aspartate, choline, creatine, glutamate/glutamine (Glx), gamma-aminobutyric acid (GABA), glutathione, and myoinositol can be reliably quantified. MRS has diverse applications in investigating the metabolic window of a wide range of biochemical processes. Here, we have utilized MRS to better understand chemical changes associated with neurological disorders and treatment response. We have investigated neurometabolic imbalances in brain regions related to post-traumatic stress disorder (PTSD) symptoms and found that neurometabolic changes are brain region-specific in PTSD population compared to healthy controls. MRS was applied to better understand the neurobiological processes of hyperbaric oxygen therapy in military veterans with clinically diagnosed traumatic brain injury and/or PTSD. From preliminary data, we found the largest neurometabolic changes in the insula – a core component of body awareness. Lastly, MRS was utilized to understand neurochemical changes during and following transcranial Direct Current Stimulation (tDCS) for modulation of brain activity in a large healthy cohort. Increases in primary inhibitory neurotransmitter GABA, energy marker creatine, and excitatory neurotransmitter Glx were observed. With improved specificity and the ability to probe microstructural and chemical changes, MRS represents a powerful tool for investigating various disorders and treatment responses. Zoom link: https://purdue-edu.zoom.us/j/98032847567 BME Distinguished Seminar Series, Wed., April 12, 9:30 a.m. MJIS 1001 and via Zoom. Chien-Chi Lin, Thomas J. Linnemeier Guidant Foundation Endowed Chair and Professor of Biomedical Engineering, Indiana University-Purdue University Indianapolis will present “Click hydrogels for pancreatic tissue engineering.” Abstract: Hydrogels are hydrophilic polymer networks capable of imbibing large amount of water without dissolving. Hydrogels have a long history in biomedical applications, initially being used as a bioinert and non-fouling material for contact lenses nearly 60 years ago. In the last two decades, innovative and smart hydrogels are being explored as scaffolds for cell and drug delivery to treat diseases and as platforms to study cell biology in three-dimension (3D). Sing Aug. 2010, the Lin Research Group at IUPUI has being designing biomimetic and dynamic hydrogels for cancer and stem cell biology. We leverage diverse click chemistries for designing polymeric hydrogels with controllable biophysical (e.g., stiffness, degradability, permeability, viscoelasticity, oxygen content, etc.) and biochemical properties (e.g., cell signaling motifs and degradation sites). We have developed hydrogels at various length scales for answering important biological questions related to the development of cancer and stem cells, for releasing therapeutically relevant agents to facilitate disease treatment, and for delivering adult and stem/progenitor cells to accelerate tissue regeneration. In this talk, I will highlight our recent progress in the development of orthogonally crosslinked, well-defined, and dynamic hydrogels for studying pancreatic cancer cell fate and for guiding pancreatic differentiation of induced pluripotent stem cells (iPSCs). Bio: Chien-Chi Lin is the Thomas J. Linnemeier Guidant Foundation Endowed Chair & Professor of Biomedical Engineering at Indiana University-Purdue University Indianapolis (IUPUI). He joined IUPUI as an Assistant Professor of Biomedical Engineering in August 2010 and rose through the ranks to Associate Professor in 2016 and to Professor in 2020. Prior to joining IUPUI, he was a Howard Hughes Medical Institute (HHMI) postdoctoral fellow at the University of Colorado Boulder and obtained his PhD degree in Bioengineering in 2007 from Clemson University. Dr. Lin received his BS and MS degrees, both in Chemical Engineering, from Taiwan’s National TsingHua University (NTHU) and National Taiwan University (NTU), respectively. Dr. Lin’s research encompasses materials science and engineering, bioconjugation chemistry, and cancer/stem cell biology. Dr. Lin has been recognized throughout his academic career, including Outstanding Graduate Student Awards (Clemson University), American Institute of Chemists Postdoctoral Award (CU-Boulder), Georgia Tech Frontiers in Bioengineering Young Investigator Award, NSF Faculty Early Career Development (CAREER) Award, National Academy of Engineering Frontiers of Engineering Symposium Invitee, Abraham M. Max Distinguished Professor Award (IUPUI Purdue School of Engineering & Technology), and served as an Honorary Scientist & Advisor on Agricultural Science & Technology for Rural Development Administration in Republic of Korea.​ Since 2011, Dr. Lin’s research group has been continuously supported by federal agencies, including NIH, NSF, and US Army. He also received funding supports from foundation, industry, and international agencies. ~ BME Host: Young Kim ~ Zoom link: https://purdue-edu.zoom.us/j/5593290378?pwd=eFBOZFlNTU50ZDA2S2gwcnpyOWIwUT09 NOTE: Students registered for the seminar are expected to attend in-person. BME-IBSC PhD Defense Announcement for Jennifer L. Anderson, Wed., April 12, 12:30 p.m., MJIS 2001 and via Zoom. Title: The Role of Renal Compartment Syndrome in renal injury during pregnancy Thesis Committee Members: Dr. Craig J. Goergen, Chair; Dr. David G. Reuter; Dr. Abigail Cox; Dr. Bruno Roseguini; Dr. George R. Wodicka Abstract: Preeclampsia and other hypertensive disorders of pregnancy impact 2-8% of pregnancies with often devastating results. Current treatment methods resort to birth, which forces the fetus into the world before they are fully developed but can save the mother’s life. Preeclampsia is broadly considered to be of placental origin and current etiologic understanding focuses on systemic endothelial dysfunction triggered by an imbalance of vasoregulatory factors released by this maternal/fetal organ. This imbalance explains many early-term cases but fails to adequately address later cases where this imbalance is not always seen. Conversely, ischemia-reperfusion of the kidney is known to correlate with endothelial dysfunction, and preeclamptic women are known to have a stenosis in their left renal vein (LRV) in the supine position (on their back). Herein, we suggest that extrinsic compression of the LRV by the gravid uterus, without collaterals, produces a renal injury which can induce systemic endothelial cell dysfunction. We theorize this compression is position dependent and produces renal ischemia through an unchecked cycle of increased intrarenal pressure, subsequent afferent arteriole constriction and decreased glomerular perfusion, and activation of the renin-angiotensin-aldosterone system. We aim to elucidate this through murine studies of a surgically induced LRV stenosis and a retrospective clinical study where the maternal renal veins are measured from magnetic resonance images. Findings from this work suggest partial renal venous outflow obstruction leads to renal injury but could be moderated through alternative maternal resting positions. This potential alternative pathologic mechanism has significant clinical implications for future therapies targeting this condition. Zoom link: https://purdue-edu.zoom.us/j/97092123267?pwd=OWdUeXhZUklUeHRPYTFyQ1FaV2hqUT0... BME PhD Defense Announcement for Ángel G. Enríquez Mujica, Thurs., April 13, (H. Lee, advisor), 9:00 a.m., MJIS 2001 and via Zoom. Title: Magnetic actuators for applications in biomedical devices and a novel thrombectomy catheter. Everyone is invited to attend the public presentation. Thesis Committee: Hyowon (Hugh) Lee (Major professor), Luis Solorio, Chi-Hwan Lee, Daniel Sahlein Abstract: The untethered transfer of energy and the scalability of magnetic actuators can add novel functionalities to an otherwise passive system. For example, wireless magnetic actuation can turn static 2D and 3D cell cultures into a more physiologically-relevant dynamic environment that can limit contamination. Moreover, magnetic microactuators can turn conventionally static indwelling catheters and implantable sensors with limited functional lifespan into more active and reliable medical devices. In this work, I will demonstrate examples of novel biomedical microdevices enabled by magnetic actuation. First, I will describe a soft polymer magnetic actuator that can facilitate the study of a physiologically relevant cell culturing system. By cyclically stretching an extracellular matrix protein in a 3D cell culture, this system can elucidate the process by which breast cancer cells respond to a dynamic environment in the lungs. Our results demonstrate a clear suppression of cancer cell progression due to cyclic stretching that has implications for a mechanical role in the dormancy and reactivation of metastasizing breast cancer cells. As a second application, I will demonstrate the use of magnetic microactuators to regenerate biosensors to prolong their functionality. Our preliminary results suggest that the motion of the actuator on the sensor surface can maintain biosensor signal integrity and prevents the downstream effects of the foreign body response. Finally, I will present the design and proof of concept testing of a novel aspiration thrombectomy catheter meant to improve the engagement between the catheter and the blood clot being removed. Our preliminary results demonstrate the added benefit of incorporating a microstructure in an aspiration catheter lumen with increased embolism retraction force. Zoom link: https://purdue-edu.zoom.us/j/98595923252 ; Meeting ID: 985 9592 3252 BME PhD Defense Announcement for Peiyi Zhang (Fang Huang, advisor), Tues., April 18, 10:00 a.m., MJIS 2001 and via Zoom. Title of Thesis: Single molecule analysis and wavefront control driven by deep learning. Everyone is invited to attend the public presentation beginning at 10:00 a.m. Thesis Committee members: Fang Huang (chair), Eugenio Culurciello, Charles Bouman, Daniel Suter, David Umulis, Tongcang Li Abstract: Analyzing single molecule emission patterns plays a critical role in retrieving the structural and physiological information of their tagged targets, and further, understanding their interactions and cellular context. These emission patterns of tiny light sources (i.e. point spread functions, PSFs) simultaneously encode information such as the molecule’s location, orientation, the environment within the specimen, and the paths the emitted photons took before being captured by the camera. However, retrieving multiple classes of information beyond the 3D position from complex or high-dimensional single molecule data remains challenging, due to the difficulties in perceiving and summarizing a comprehensive yet succinct model. We developed smNet, a deep neural network that can extract multiplexed information near the theoretical limit from both complex and high-dimensional point spread functions. Through simulated and experimental data, we demonstrated that smNet can be trained to efficiently extract both molecular and specimen information, such as molecule location, dipole orientation, and wavefront distortions from complex and subtle features of the PSFs, which otherwise are considered too complex for established algorithms. Single molecule localization microscopy (SMLM) forms super-resolution images with a resolution of several to tens of nanometers, relying on accurate localization of molecules’ 3D positions from isolated single molecule emission patterns. However, the inhomogeneous refractive indices distort and blur single molecule emission patterns, reduce the information content carried by each detected photon, increase localization uncertainty, and thus cause significant resolution loss, which is irreversible by post-processing. To compensate tissue induced aberrations, conventional sensorless adaptive optics methods rely on iterative mirror-changes and image-quality metrics to compensate aberrations. But these metrics result in inconsistent, and sometimes opposite, metric responses which fundamentally limited the efficacy of these approaches for aberration correction in tissues. Bypassing the previous iterative trial-then-evaluate processes, we developed deep learning driven adaptive optics (DL-AO), for single molecule localization microscopy (SMLM) to directly infer wavefront distortion and compensate distortion near real-time during data acquisition. our trained deep neural network monitors the individual emission patterns from single molecule experiments, infers their shared wavefront distortion, feeds the estimates through a dynamic filter (Kalman), and drives a deformable mirror to compensate sample induced aberrations. We demonstrated that DL-AO restores single molecule emission patterns approaching the conditions untouched by specimen and improves the resolution and fidelity of 3D SMLM through brain tissues over 130 µm, with as few as 3-20 mirror changes. Zoom link: https://purdue-edu.zoom.us/j/92016185985 BME PhD Preliminary Exam Announcement for Kentaro Umemori (D. Little, advisor), Mon., April 17. Everyone is invited to attend the public presentation beginning at 9:30 AM EST in MJIS 2001. Research Title: The Impact of Polymer on 3D Meltblown Scaffolds and the Epigenome of Adipose-Derived Progenitors. Thesis Committee Members: Dr. Dianne Little D.V.M. Ph.D. (Chair); Dr. Deva Chan Ph.D.; Dr. Luis Solorio Ph.D.; Dr. Vikki Weake Ph.D. Abstract: Over 460,000 rotator cuff tendon tears occur annually in the US, but even after surgery, the retear rate is up to 90% for massive tears. To augment the repair process, tendon tissue engineering approaches seek to improve these outcomes using biomimetic scaffolds. Currently, electrospinning is a common strategy to fabricate scaffolds. However, this process is difficult to translate due to difficulties in scalability and repeatability. This led to the development of 3D meltblowing (3DMB) which introduces a robotic collector that can produce scaffolds at high speeds with highly organized fibers. My preliminary data demonstrate cell proliferation and collagen deposition in 3DMB poly-L-lactic acid and poly-ε-caprolactone scaffolds cultured with adipose derived stem cells (ASCs). However, there were substantial differences in neo-collagen alignment and tendon-related proteomic datasets between polymers. These disparities suggest that biophysical and biochemical cues related to the polymer type are the underlying cause. However, how these cues promote transition of the ASC epigenome towards those of tendon fibroblasts in response to biomaterials is unknown. Therefore, my overall hypothesis is that the specific polymer used for production of 3DMB scaffolds is critical for promotion of a tendon-related epigenetic landscape in ASCs. To test this hypothesis, I propose the following Aims. Aim 1: I will perform epigenetic profiling of ASCs and tendon fibroblasts to pinpoint the epigenetic markers related to tendon. Aim 2: I will perform a polymer screen and epigenetic analysis of 3DMB scaffolds to identify the impact of polymer type on tendon-related epigenetic marks. Collectively, this study will provide a template for the modification of epigenetic processes to stimulate ASC differentiation to tendon fibroblasts and will significantly advance the understanding of the influence of polymer type on the epigenome during engineered tendon development. BME Master's Defense Announcement for Pankti R. Thakkar (J. Linnes, advisor), Tues., April 18, 12:00 p.m., BRK 2001 and via Zoom. Thesis Title: Characterization and Development of an Enzymatically Signal Enhanced Lateral Flow Assay Test for HIV using p24 Antigen. Everyone is invited to attend the public presentation beginning at 12:00 pm. Thesis Committee: Jacqueline Linnes, PhD, Chair; Lia Stanciu, PhD; Scott Bolton, PhD Abstract: In 2021, an estimated 1.5 million people were diagnosed with HIV globally, increasing the total to 38.4 million people. Approximately 16% of this population were unaware of their infected status and required HIV testing, which is a critical first step in HIV prevention, treatment, and care [1]. Hence, there is a need to develop a rapid, user-friendly, and cost-effective point-of-care test for HIV detection. The time between HIV infection and a detectable host HIV antibody concentration can extend up to 90 days. By incorporating more sensitive testing for the HIV p24 antigen on the virus, the diagnosis lag can be reduced to 17 days [2]. This window could be further shortened by using horseradish peroxidase (HRP) enzyme as a signal enhancement technique. The work herein focuses on developing an enzymatically signal-enhanced lateral flow assay test for the p24 antigen to detect HIV during the acute phase of infection. Conjugation chemistry for the sandwich assay was characterized using DLS and UV-Vis. Dot blots were then used to assess and enhance the functionality of the individual components via a visual color gradient formed by the protein coupled with antibody-conjugated gold nanoparticles. A quantitative analysis was performed using ImageJ software through signal pixel intensity analysis. A limit of detection (LoD) of 6 ng/mL was obtained for the detection of p24 antigen in the patient sample. This LoD was improved to 0.2 ng/mL by incorporating HRP signal enhancement with diaminobenzidine substrate [3]. This 30x signal improvement could drive down the LoD even further to improve the sensitivity of the commercial p24 antigen tests. Different fabrication and scalability studies were performed to produce a cost-efficient, fully functional prototype of a paper-based lateral flow device incorporating the signal-enhanced p24 assay. This study serves as a solid foundation to research focused on creating more efficient point-of-care tests that can be used in resource-limited settings to provide early detection of HIV for the 6 million individuals who are currently unaware of their HIV status. [1] October 25, 2021, “Global HIV/AIDS Organizations.” [2] “Types of HIV tests, HIV basics | HIV/AIDS | CDC.” [3] KM. Byers et al., ACS Omega 5, no. 9 (March 10, 2020): 4673-81 Zoom Link: https://purdue-edu.zoom.us/j/8181492040 BME Master's Defense Announcement for Riley J. Brown (J. Linnes, advisor). Thurs., April 20. Everyone is invited to attend the public presentation beginning at 12:00 pm. In BRK 1001 and via Zoom. Title: Characterization and Implementation of Screen Printed, Flexible PTC Heaters for Portable Diagnostic Testing Thesis Committee: Dr. Jacqueline Linnes (chair), Dr. Lia Stanciu, and Dr. Orlando Hoilett Abstract: The 2020 pandemic emphasized the need for accessible and accurate point-of-care diagnostic tests. With the continued development of isothermal nucleic acid amplification tests, this can be achieved. A requirement of these tests includes heating and holding a specific temperature, in this case, 65℃ for 30 minutes, for amplification to occur. To achieve this, heaters often require external feedback to control the temperature; bringing up the device’s cost. Several self-regulating heaters have been made with materials having a positive thermal coefficient of resistance eliminating the need for complex circuitry. With this property, point-of-care diagnostic tests can be simplified and made more accessible. In this study, ink-based positive thermal coefficient of resistance heaters are developed and characterized using the scalable method of screen printing to achieve 65℃ and aid in the detection of SARS-CoV-2. Various curing methods and screen-printing parameters were evaluated to improve the stability and understanding of the reproducibility of the heaters. The longevity of the heaters was evaluated with oxidation studies and a COMSOL model was created to study the heat transfer within the device. Furthermore, the heaters were successfully implemented into a second-generation electronic point-of-care diagnostic device. Detection of SARS-CoV-2 using a self-regulating heater removes the need for complex circuitry, improving the accessibility of point-of-care tests with the potential to be expanded to a wide range of pathogen detection. Zoom link: https://purdue-edu.zoom.us/j/95178617829?pwd=QnlmMmIrZU9zWWNqZ2I2eFNaMW9Edz0... Participants Needed for Research Study: (Aditya Shanghavi is a graduate student in Biomedical Engineering at Purdue.) I am currently working on a research project under the direction of my advisor, Dr Anne Sereno. We are inviting you to participate in a brief arm movement study. We are specifically looking for participants aged 45-85 with no history of neurological diseases who are fluent in English and have adequate hearing (with or without amplification) to process verbal instruction and adequate vision to see the computer screen. Participants should be able to perform simple upper arm motions without experiencing major pain or discomfort. We will use non-invasive motion sensors on a wristband to collect arm motion signals during normal and simulated abnormal movements (after viewing short videos) as well as an eye tracker to collect eye motion signals during a prosaccade-antisaccade task. This study's goal is to develop algorithms to detect latencies and amplitudes of movements and to quantify the sensitivity, reliability, and accuracy of the sensors and these algorithms to differentiate normal versus simulated abnormal movements. The study should take no more than 60 minutes and it will be conducted in PSYCH 3125. We will highly appreciate you taking time out of your busy schedule and will compensate you $15 for your help in our research study in addition to our heartfelt gratitude. This study is conducted in accordance with IRB # IRB-2022-1299. If you have more questions regarding this study, please contact Aditya Shanghavi at ashangha@purdue.edu<mailto:ashangha@purdue.edu>. Diabetes Data and Hypothesis Hub (D2H2) Seminar, Fri., May 12, 12:00 p.m., via Zoom. Dr. Avi Ma’ayan will present "The Diabetes Data and Hypothesis Hub (D2H2) and the Playbook Partnership Workflow Builder (PPWB): Bioinformatics Tools for Hypothesis Generation via Data Integration.” Register here: https://scgcorp.zoom.us/webinar/register/WN_YgWw6WDTRX6TfaugZ3OBXg Abstract: This seminar will discuss two new interactive bioinformatics software tools that integrate knowledge from many NIH funded and other key resources for hypothesis generation. The Diabetes Data and Hypothesis Hub (D2H2) is a platform that integrates tools and datasets related to diabetes research into one integrative platform. The Playbook Partnership Workflow Builder (PPWB) is a system that enables novice users to created bioinformatics workflows by exploring a network of distributed but connect microservices. These two new tools will be demonstrated through a variety of use cases with applications that prioritize personalized drugs and targets for kidney disease, diabetes, and pan-cancer. Bio: Dr. Ma’ayan is a Mount Sinai Endowed Professor in Bioinformatics, Professor in the Department of Pharmacological Sciences, Director of the Mount Sinai Center for Bioinformatics, and a faculty member of the Department of Artificial Intelligence and Human Health at the Icahn School of Medicine at Mount Sinai in New York City. The Ma'ayan Laboratory applies computational methods to study the complexity of regulatory networks in mammalian cells. His research team develops software systems to help experimental biologists form novel hypotheses from high-throughput data with a focus on drug and target discovery through machine learning and other statistical methods. New Course for Fall 2023: BME 69500 Pediatric Medical Devices (CRN 14011). Fall 2023, 3.0 credit hours Wednesdays 11:30-2:20 in MJIS 1083 (in person attendance only) Prerequisite: BME Graduate Standing Course Description: The unique medical needs of pediatric patients in the context of current and emerging clinical technologies are presented in this novel course. Anatomical, physiological, and pathophysiological underpinnings of diseases in infants and children are studied. Unique aspects of pediatric device design, regulation, and translation, including clinical studies and ethical considerations, are explored. Numerous clinical sub specialties such as cardiology, surgery, nephrology, pulmonology, and gastroenterology are examined in terms of technology adoption and remaining clinical needs. Future directions in device development and emerging trends in patient care are considered throughout. Weekly Course Topics: August 23 Pediatrics Introduction August 30 Neonatology Introduction September 6 Pediatric Device Design and Development I September 13 Pediatric Device Design and Development II September 20 Pediatric Device Regulation September 27 Pediatric Clinical Studies October 4 Pediatric Cardiology October 18 Pediatric Cardiovascular Surgery October 25 Pediatric Nephrology November 1 Pediatric Pulmonology November 8 Pediatric Wound Healing November 15 Pediatric Gastroenterology November 29 Ethical Considerations in Pediatrics December 6 Future Directions and Trends Primary Instructor: George Wodicka, MJIS 3036, wodicka@purdue.edu Numerous experts from the Indiana University School of Medicine Department of Pediatrics, Riley Hospital for Children, and Cook Medical, Inc. will actively participate. Spring 2023 BME Seminar Schedule (Wednesdays, 9:30 a.m. in MJIS 1001) Week Date Speaker Host Week 13 4/12/2023 Dr. Chien-Chi Lin Thomas J. Linnemeier Guidant Foundation Endowed Chair & Professor of BME at IUPUI Dr. Young Kim Week 14 4/19/2023 Dr. Rachel Surowiec Assistant Professor of BME at IUPUI Dr. Young Kim Week 15 4/26/2023 Dr. James Moon J. G. Searle Professor, Pharmaceutical Sciences Professor, Biomedical Engineering at Michigan Dr. Chi Hwan Lee The titles and abstracts of the talks will be added here: https://purdue.edu/TPAN/hearing/shrp_abstracts<http://ogww.mj.am/lnk/AU8AAEukm7AAAchk2HAAALFJlZgAAYCsFQMAnDnUAATD7ABh_T90ybQipdUVQZ20y9CAzjYFPgAEkSo/6/a2vUEcd_J6SQbZdR8Ley5Q/aHR0cHM6Ly9wdXJkdWUuZWR1L1RQQU4vaGVhcmluZy9zaHJwX2Fic3RyYWN0cw> Attachments Course Info Form 2023 Article_Faculty Well-Being.Roos.Borkoski.2021 Purdues Personal and Academic Help Resources -- Bmeroundtable-list mailing list Bmeroundtable-list@ecn.purdue.edu https://engineering.purdue.edu/ECN/mailman/listinfo/bmeroundtable-list