BME PhD Defense Announcement for Ziqian Zeng (N. Alves and S. Harbin, co-advisors) Everyone is invited to attend the public presentation beginning at 9:00 AM EST. Title: Developing in-vitro Synthetic Blood Clot Models for Testing Thrombolytic Drugs Date: April 11, 2022 Time: 09:00 AM EST Location: Link: https://purdue-edu.zoom.us/j/95875501047?pwd=VkVZMXlMSkJWOHpWWWFZUDFYbG9jdz0... Meeting ID: 958 7550 1047 Passcode: 636182 Committee: Dr. Nathan J Alves (Advisor, Co-Chair) Dr. Sherry L. Harbin (Co-Chair) Dr. Julie C. Liu (Member) Dr. Vitaliy L. Rayz (Member) Abstract: Thrombosis is the pathological blood clot formation that blocks blood circulation, leading to high morbidity and mortality rates. Thrombolytic drugs that offer rapid clot dissolution are promising treatments, yet current drugs are associated with limited efficacy and high bleeding risks. While numerous animal thrombosis models have been developed for drug screening, the translation of therapeutic agents into and through clinical trials remains limited. This is primarily due to animal models’ poor reproducibility and distinctive physiology to that of humans. In vitro flow models that utilize both human blood components and physiologically relevant flow conditions can provide for a more representative testing environment to screen drugs. Developing better in vitro models may not eliminate the need for preclinical animal testing but can help exclude inefficient agents earlier in the drug development pipeline to expedite the drug evaluation process. Existing in vitro thrombolysis flow models are not ideal as they either adopt over-simplified clot substrates or utilize small-length-scale geometries that insufficiently mimic native hemodynamics. Thus, we propose to develop a highly reproducible, physiological scale, flowing clot lysis model to screen thrombolytic drugs. This model combines an in vivo-like fluorescence incorporated synthetic clot (FISC) and a human-relevant flow system. This system is a tubing-based structure comprising a peristaltic pump, a dampener, and a fluorometer to offer real-time monitoring of thrombolysis under steady or pulsatile flow. The synthetic clot model can mimic various thrombosis conditions and offer representative drug testing and dosing results across numerous thrombolytic agents. ---------------------------- Ziqian Zeng is inviting you to a scheduled Zoom meeting. Topic: Ph.D. Final Exam for Ziqian Zeng Time: Apr 11, 2022 09:00 AM EST Join Zoom Meeting https://purdue-edu.zoom.us/j/95875501047?pwd=VkVZMXlMSkJWOHpWWWFZUDFYbG9jdz0... Meeting ID: 958 7550 1047 Passcode: 636182 One tap mobile +13017158592,,95875501047#,,,,*636182# US (Washington DC) +13126266799,,95875501047#,,,,*636182# US (Chicago) Dial by your location +1 301 715 8592 US (Washington DC) +1 312 626 6799 US (Chicago) +1 646 558 8656 US (New York) +1 253 215 8782 US (Tacoma) +1 346 248 7799 US (Houston) +1 669 900 6833 US (San Jose) Meeting ID: 958 7550 1047 Passcode: 636182 Find your local number: https://purdue-edu.zoom.us/u/aeDmWdfSjo