[cid:b9a695e0-2f7e-4f46-8e32-5eecfc0fd297] Dear EMBRIO Members, Tune in this afternoon 3-4pm EST for our invited seminar by Scott Howard<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fengineering.nd.edu%2Ffaculty%2Fscott-howard%2F&data=05%7C02%7Cembrio-list%40ecn.purdue.edu%7C60cb37779fee46d886cc08dd3ee48cc1%7C4130bd397c53419cb1e58758d6d63f21%7C0%7C0%7C638735873510144497%7CUnknown%7CTWFpbGZsb3d8eyJFbXB0eU1hcGkiOnRydWUsIlYiOiIwLjAuMDAwMCIsIlAiOiJXaW4zMiIsIkFOIjoiTWFpbCIsIldUIjoyfQ%3D%3D%7C0%7C%7C%7C&sdata=CZL%2B2YajJwKb5u7zJ4jSLOk%2B21t0Z4zhs264FUmPEm0%3D&reserved=0>, Ph.D., University of Notre Dame "Next Generation Fluorescence Lifetime Imaging Microscopy (FLIM)" Zoom link: https://purdue-edu.zoom.us/j/97946412458?pwd=YUurFKck0n1am07eA7b0O0TVKsVwpC.1&from=addon<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fpurdue-edu.zoom.us%2Fj%2F97946412458%3Fpwd%3DYUurFKck0n1am07eA7b0O0TVKsVwpC.1%26from%3Daddon&data=05%7C02%7Cembrio-list%40ecn.purdue.edu%7C60cb37779fee46d886cc08dd3ee48cc1%7C4130bd397c53419cb1e58758d6d63f21%7C0%7C0%7C638735873510165380%7CUnknown%7CTWFpbGZsb3d8eyJFbXB0eU1hcGkiOnRydWUsIlYiOiIwLjAuMDAwMCIsIlAiOiJXaW4zMiIsIkFOIjoiTWFpbCIsIldUIjoyfQ%3D%3D%7C0%7C%7C%7C&sdata=iDwbcFomcueRiXU7shxC4bueV5nZR1FHQATEkOW2x7I%3D&reserved=0> Abstract This talk will introduce fluorescence lifetime imaging microscopy (FLIM), a microscopy tool for quantitative analysis of intravital microenvironments, and our "Instant FLIM" system that costs a fraction of existing multiphoton FLIM systems and achieves the highest imaging rate, depth-resolved, intravital imaging in highly scattering tissue. Our open-source image analysis and processing tools, including neural-network based image denoising, and our work developing the first theoretical framework for machine-learning based FLIM image processing and analysis will be presented. The Spring Seminar schedule, Monday's 3-4pm ET (please contact Jennifer Goad: goad3@purdue.edu for any changes needed in scheduling): Month Day Spring Semester 2025 Jan 13 Denise Driscoll, Purdue (ABIDES Invited Scholar) Recording<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fapp.box.com%2Fs%2F7jk3rk3bizsfr563f1g8r6actavp262i&data=05%7C02%7Cembrio-list%40ecn.purdue.edu%7C60cb37779fee46d886cc08dd3ee48cc1%7C4130bd397c53419cb1e58758d6d63f21%7C0%7C0%7C638735873510177104%7CUnknown%7CTWFpbGZsb3d8eyJFbXB0eU1hcGkiOnRydWUsIlYiOiIwLjAuMDAwMCIsIlAiOiJXaW4zMiIsIkFOIjoiTWFpbCIsIldUIjoyfQ%3D%3D%7C0%7C%7C%7C&sdata=gRSBSbnHm%2BfpHds9LAIFgaV0%2BRbEySH6Z6CL%2Ftkmv98%3D&reserved=0> Jan 27 Scott Howard, Notre Dame Feb 10 Tami Kinzer-Ursem; Thrust 1A Feb 24 Janice Evans; Thrust 1B Mar 10 Chris Staiger and Anjali Iyer-Pascuzzi; Thrust 2A Mar 24 Jeremiah Zartman; Thrust 2B Apr 7 Qing Deng; T3B Apr 21 Mary Mullins; T3A May 5 Stephanie Gardner; T4 May 19 Ale Magana (Joreen/Abby); T4 Get Your Summer Intern Projects Submitted Now for the SURF Program! Reminder to faculty at Purdue (and Partners - please identify any undergrads at your university you might want to send to Purdue to work in EMBRIO labs for the summer): Submit your EMBRIO project idea now to take advantage of the EMBRIO-SURF collaboration<https://engineering.purdue.edu/Engr/Research/EURO/info-faculty> to bring excellent undergraduate students into your lab this summer. EMBRIO Institute will cover the faculty mentor portion, along with the $1000 housing requirement for external students. Please see the SURF program project submittal page linked above for details, and make sure to check the EMBRIO Institute box. Kay Kobak, SURF Asst. Dir., can help with any programmatic, student matching, or project submission questions: kkobak@purdue.edu Recent EMBRIO Publications: Check out the new pre-prints on bioRxiv from Thrust 2A with the Staiger Lab and a collaborative effort with five EMBRIO labs involved : Xu, L., W. Zhang, L. Huang, C. Zhang, and C.J. Staiger. 202X. Actomyosin and the Arp2/3 complex are involved in the internalization of cellulose synthase complexes. Plant Physiol. Submitted 01-17-25. Also available on bioRxiv at https://doi.org/10.1101/2025.01.19.633783<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.1101%2F2025.01.19.633783&data=05%7C02%7Cembrio-list%40ecn.purdue.edu%7C60cb37779fee46d886cc08dd3ee48cc1%7C4130bd397c53419cb1e58758d6d63f21%7C0%7C0%7C638735873510188974%7CUnknown%7CTWFpbGZsb3d8eyJFbXB0eU1hcGkiOnRydWUsIlYiOiIwLjAuMDAwMCIsIlAiOiJXaW4zMiIsIkFOIjoiTWFpbCIsIldUIjoyfQ%3D%3D%7C0%7C%7C%7C&sdata=undTzjOIQX4eyFcg%2BFry3r25G7NWz6FGaRw2Gv7eHeo%3D&reserved=0> Zhang, W., N. Kumar, J. Helwig, A. Hoerter, D. Umulis, A.S. Iyer-Pascuzzi, E. Pienaar, and C.J. Staiger. 202X. Local traveling waves of cytosolic calcium elicited by defense signals or wounding are propagated by distinct mechanisms. Science Signaling. Submitted 01-23-25. Also available on bioRxiv at https://doi.org/10.1101/2025.01.23.634554<https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.1101%2F2025.01.23.634554&data=05%7C02%7Cembrio-list%40ecn.purdue.edu%7C60cb37779fee46d886cc08dd3ee48cc1%7C4130bd397c53419cb1e58758d6d63f21%7C0%7C0%7C638735873510200490%7CUnknown%7CTWFpbGZsb3d8eyJFbXB0eU1hcGkiOnRydWUsIlYiOiIwLjAuMDAwMCIsIlAiOiJXaW4zMiIsIkFOIjoiTWFpbCIsIldUIjoyfQ%3D%3D%7C0%7C%7C%7C&sdata=FlTGALMi9OOQK%2FCXg4XWU5WiJ3m3718p13chWaefpYs%3D&reserved=0> Let me know of your recent publications and pre-print posts, as well as conference papers and posters. Access the Institute's Google Scholar page and full list of products on our website: https://www.purdue.edu/research/embrio/research/scholarly-products.php Brent T. Ladd, Senior Research Program Manager, EMBRIO Institute<https://www.purdue.edu/research/embrio/> Weldon School of Biomedical Engineering, Purdue University Office: Hall for Discovery Learning and Research, Ste. 203 207 S. Martin Jischke Drive West Lafayette, IN 47907 laddb@purdue.edu