This talk will introduce fluorescence lifetime imaging microscopy (FLIM), a microscopy tool for quantitative analysis of intravital microenvironments, and our "Instant FLIM" system that costs a fraction of existing multiphoton FLIM systems and achieves the highest imaging rate, depth-resolved, intravital imaging in highly scattering tissue. Our open-source image analysis and processing tools, including neural-network based image denoising, and our work developing the first theoretical framework for machine-learning based FLIM image processing and analysis will be presented.

Reminder to faculty at Purdue (and Partners - please identify any undergrads at your university you might want to send to Purdue to work in EMBRIO labs for the summer): Submit your EMBRIO project idea now to take advantage of the EMBRIO-SURF collaboration to bring excellent undergraduate students into your lab this summer. EMBRIO Institute will cover the faculty mentor portion, along with the $1000 housing requirement for external students. Please see the SURF program project submittal page linked above for details, and make sure to check the EMBRIO Institute box. Kay Kobak, SURF Asst. Dir., can help with any programmatic, student matching, or project submission questions: kkobak@purdue.edu 

 

Zhang, W., N. Kumar, J. Helwig, A. Hoerter, D. Umulis, A.S. Iyer-Pascuzzi, E. Pienaar, and C.J. Staiger. 202X. Local traveling waves of cytosolic calcium elicited by defense signals or wounding are propagated by distinct mechanisms. Science Signaling. Submitted 01-23-25. Also available on bioRxiv at https://doi.org/10.1101/2025.01.23.634554