NSAC Coffee Hour talk tomorrow on Cryo TEM
Dear All, This year, Jacques Dubochet, Joachim Frank, and Richard Henderson— won the Nobel prize in Chemistry for their work on developing *Cryo-Electron Microscope*. I am happy to announce that our next NSAC Coffee hour topic would cover work by Christopher on Cryo TEM. Please see below for title and abstract of his talk. *Also, we have GSOGA funding, so we will have special snacks for tomorrow's coffee hour.* *What: NSAC Coffee Hour talk* ** *When: Friday, November 3rd, 2017 3:30 pm* *Where: BNC Atrium* *Speaker: **Christopher J Benjamin* Topic: Development of Techniques Using Novel Bioorganic Materials for Protein Structure Determination ABSTRACT: Single-particle reconstruction has grown significantly with the improvements in various data collection and computational strategies including CTF fitting, the use of vitrified samples and the utilization of ultra-sensitive direct electron detectors. Although these improvements have contributed significantly to the recent evolution of 3D reconstruction analysis, the way samples are prepared for electron microscopy has remained largely unchanged. We report the development of TEM grids that are modified with non-fouling coatings bearing surface grafted nitrilotriacetic acid substituents that promotes specific capture of protein targets for high-resolution TEM analysis. The utilization of these grids for specific adsorption of the targeted protein onto the grid surface results in well-controlled surface concentration enhancements and a days-to-minutes reduction in time required for the preparation of a purified sample for cryoEM analysis from an *E. coli* expression system. The selective and reversible capture of his-tag T7 bacteriophage, RplL, and GroEL from crude lysates, as well as purified nanodisc-solubilized his-malFGK2, on these NTA-modified grids with an exceptionally low level of adsorption by non-target proteins, has been observed. Our data illustrate the utility of these grids for selective capture from complex mixtures, detergent-solubilized membrane protein isolates, and expression systems yielding low copy numbers of the desired target in a manner that is well-suited for single particle reconstruction analysis. Also, please feel free to sign up for the Spring coffee hour slots in the Google sheet <https://mailtrack.io/trace/link/7fe51a6c721d6d80a12f5662bf5f73ed9de80dc0?url=https%3A%2F%2Fsecure-web.cisco.com%2F1rmAyqOB_LXn787ekSYOr1rB_ZSfisYeSx7VsOZKsCYZmWOxgIdopXXn0Wyu7qkXEvb9fHcms2fnHt8pkjrHKXhRsz-FBYfWvh5w3MwkwbmER0matVxIiklSxeXjrfjk5TTmrvhYfOT5T0iz41DTG2pUZfBh8AF7_ARaCqH1k0ZrvfHb4kMacI9kKcucLBHPUEbXCkCyHuW8glNf2ruVl3vnhLfBbsosN4eI9w4xXC4OfRPRGq7bs0-Tzp_CB6Q2F2oT1-GKpYPSWE4KSLY2_veObmUDHIGY-xuapEdUS-29c91ikOZCSPD-0Ba0-YE8Wc7JJZWwHXAIM2_rQRIvn2XHy4oZ-UjtaUKETvgscYdl83MXiBzTNRCFlxsotjALt%2Fhttps%253A%252F%252Fgoo.gl%252F7w24Au&userId=2154587&signature=89dfc57c0f626d8d> by clicking here <https://mailtrack.io/trace/link/6f43651d269a915008cf6b4751984f0a88b2c4f8?url=https%3A%2F%2Fsecure-web.cisco.com%2F1rmAyqOB_LXn787ekSYOr1rB_ZSfisYeSx7VsOZKsCYZmWOxgIdopXXn0Wyu7qkXEvb9fHcms2fnHt8pkjrHKXhRsz-FBYfWvh5w3MwkwbmER0matVxIiklSxeXjrfjk5TTmrvhYfOT5T0iz41DTG2pUZfBh8AF7_ARaCqH1k0ZrvfHb4kMacI9kKcucLBHPUEbXCkCyHuW8glNf2ruVl3vnhLfBbsosN4eI9w4xXC4OfRPRGq7bs0-Tzp_CB6Q2F2oT1-GKpYPSWE4KSLY2_veObmUDHIGY-xuapEdUS-29c91ikOZCSPD-0Ba0-YE8Wc7JJZWwHXAIM2_rQRIvn2XHy4oZ-UjtaUKETvgscYdl83MXiBzTNRCFlxsotjALt%2Fhttps%253A%252F%252Fgoo.gl%252F7w24Au&userId=2154587&signature=756856486ac0b824> . [image: Inline image 1] Thank you. Sajid Choudhury President Nanotechnology Student Advisory Council (NSAC) https://engineering.purdue.edu/NSAC/ <https://mailtrack.io/trace/link/f64a86a3462f87cc1469f2cb5e83ddbf9bc95065?url=http%3A%2F%2Fsecure-web.cisco.com%2F1jBfhmTyJyjIvvoQGZyMPpPPtCZwljBCnvQtKcDtrJPrkuNeXGkLs1fb65CJqsCL3dd2_IDq54FmdQzDC9mWQp_N6BJW8-RCDhP3i98B1vyX1a0isF4vAiGzw_SgO1fDGr0w6BEl-TTAeKwxbmPOgeFreDRvJhvBNCyrLviPIdYzCCpgvSlInmD_YBv6fJ1Br9hBlpfHx-2iSRci8EW5Bii2IXzhEQeq4bQ5GkwIcarWXQny14-UsabONl4tjV0ViONTSQNWQymdM03pt7TbMSPgtmJCIIRmhV3lzh3yspUZPQR_ZErp-YuUS7Yma-bqaQc_pl3mv9GHvJR062FgJ8NdN5A-IPYlzjlYKatyFpc0%2Fhttp%253A%252F%252Fgoog_878781996%252F&userId=2154587&signature=9fa76079e6de911b> <https://mailtrack.io/trace/link/f64a86a3462f87cc1469f2cb5e83ddbf9bc95065?url=http%3A%2F%2Fsecure-web.cisco.com%2F1jBfhmTyJyjIvvoQGZyMPpPPtCZwljBCnvQtKcDtrJPrkuNeXGkLs1fb65CJqsCL3dd2_IDq54FmdQzDC9mWQp_N6BJW8-RCDhP3i98B1vyX1a0isF4vAiGzw_SgO1fDGr0w6BEl-TTAeKwxbmPOgeFreDRvJhvBNCyrLviPIdYzCCpgvSlInmD_YBv6fJ1Br9hBlpfHx-2iSRci8EW5Bii2IXzhEQeq4bQ5GkwIcarWXQny14-UsabONl4tjV0ViONTSQNWQymdM03pt7TbMSPgtmJCIIRmhV3lzh3yspUZPQR_ZErp-YuUS7Yma-bqaQc_pl3mv9GHvJR062FgJ8NdN5A-IPYlzjlYKatyFpc0%2Fhttp%253A%252F%252Fgoog_878781996%252F&userId=2154587&signature=9fa76079e6de911b> and Ph.D. Candidate, School of ECE, Purdue University On Tue, Oct 24, 2017 at 1:04 PM, Sajid Choudhury <sajidmc@purdue.edu> wrote:
[image: Inline image 1] We have NSAC Coffee hour this week, Mohamed Hagag from Peroulis group will give a talk on his research.
**
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Just a reminder that we have the NSAC coffee hour at 3:30 pm today. Please see the details below: On Thu, Nov 2, 2017 at 11:19 AM Sajid Choudhury <sajidmc@purdue.edu> wrote:
Dear All,
This year, Jacques Dubochet, Joachim Frank, and Richard Henderson— won the Nobel prize in Chemistry for their work on developing *Cryo-Electron Microscope*. I am happy to announce that our next NSAC Coffee hour topic would cover work by Christopher on Cryo TEM. Please see below for title and abstract of his talk.
*Also, we have GSOGA funding, so we will have special snacks for tomorrow's coffee hour.*
*What: NSAC Coffee Hour talk* ** *When: Friday, November 3rd, 2017 3:30 pm* *Where: BNC Atrium* *Speaker: **Christopher J Benjamin* Topic: Development of Techniques Using Novel Bioorganic Materials for Protein Structure Determination
ABSTRACT: Single-particle reconstruction has grown significantly with the improvements in various data collection and computational strategies including CTF fitting, the use of vitrified samples and the utilization of ultra-sensitive direct electron detectors. Although these improvements have contributed significantly to the recent evolution of 3D reconstruction analysis, the way samples are prepared for electron microscopy has remained largely unchanged. We report the development of TEM grids that are modified with non-fouling coatings bearing surface grafted nitrilotriacetic acid substituents that promotes specific capture of protein targets for high-resolution TEM analysis. The utilization of these grids for specific adsorption of the targeted protein onto the grid surface results in well-controlled surface concentration enhancements and a days-to-minutes reduction in time required for the preparation of a purified sample for cryoEM analysis from an *E. coli* expression system. The selective and reversible capture of his-tag T7 bacteriophage, RplL, and GroEL from crude lysates, as well as purified nanodisc-solubilized his-malFGK2, on these NTA-modified grids with an exceptionally low level of adsorption by non-target proteins, has been observed. Our data illustrate the utility of these grids for selective capture from complex mixtures, detergent-solubilized membrane protein isolates, and expression systems yielding low copy numbers of the desired target in a manner that is well-suited for single particle reconstruction analysis.
Also, please feel free to sign up for the Spring coffee hour slots in the Google sheet <https://mailtrack.io/trace/link/7fe51a6c721d6d80a12f5662bf5f73ed9de80dc0?url=https%3A%2F%2Fsecure-web.cisco.com%2F1rmAyqOB_LXn787ekSYOr1rB_ZSfisYeSx7VsOZKsCYZmWOxgIdopXXn0Wyu7qkXEvb9fHcms2fnHt8pkjrHKXhRsz-FBYfWvh5w3MwkwbmER0matVxIiklSxeXjrfjk5TTmrvhYfOT5T0iz41DTG2pUZfBh8AF7_ARaCqH1k0ZrvfHb4kMacI9kKcucLBHPUEbXCkCyHuW8glNf2ruVl3vnhLfBbsosN4eI9w4xXC4OfRPRGq7bs0-Tzp_CB6Q2F2oT1-GKpYPSWE4KSLY2_veObmUDHIGY-xuapEdUS-29c91ikOZCSPD-0Ba0-YE8Wc7JJZWwHXAIM2_rQRIvn2XHy4oZ-UjtaUKETvgscYdl83MXiBzTNRCFlxsotjALt%2Fhttps%253A%252F%252Fgoo.gl%252F7w24Au&userId=2154587&signature=89dfc57c0f626d8d> by clicking here <https://mailtrack.io/trace/link/6f43651d269a915008cf6b4751984f0a88b2c4f8?url=https%3A%2F%2Fsecure-web.cisco.com%2F1rmAyqOB_LXn787ekSYOr1rB_ZSfisYeSx7VsOZKsCYZmWOxgIdopXXn0Wyu7qkXEvb9fHcms2fnHt8pkjrHKXhRsz-FBYfWvh5w3MwkwbmER0matVxIiklSxeXjrfjk5TTmrvhYfOT5T0iz41DTG2pUZfBh8AF7_ARaCqH1k0ZrvfHb4kMacI9kKcucLBHPUEbXCkCyHuW8glNf2ruVl3vnhLfBbsosN4eI9w4xXC4OfRPRGq7bs0-Tzp_CB6Q2F2oT1-GKpYPSWE4KSLY2_veObmUDHIGY-xuapEdUS-29c91ikOZCSPD-0Ba0-YE8Wc7JJZWwHXAIM2_rQRIvn2XHy4oZ-UjtaUKETvgscYdl83MXiBzTNRCFlxsotjALt%2Fhttps%253A%252F%252Fgoo.gl%252F7w24Au&userId=2154587&signature=756856486ac0b824> .
[image: Inline image 1]
Thank you.
Sajid Choudhury President Nanotechnology Student Advisory Council (NSAC) https://engineering.purdue.edu/NSAC/
Ph.D. Candidate, School of ECE, Purdue University
On Tue, Oct 24, 2017 at 1:04 PM, Sajid Choudhury <sajidmc@purdue.edu> wrote:
[image: Inline image 1] We have NSAC Coffee hour this week, Mohamed Hagag from Peroulis group will give a talk on his research.
**
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participants (1)
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Sajid Choudhury