Dear All,

This year, Jacques Dubochet, Joachim Frank, and Richard Henderson— won the Nobel prize in Chemistry for their work on developing Cryo-Electron Microscope. I am happy to announce that our next NSAC Coffee hour topic would cover work by Christopher on Cryo TEM. Please see below for title and abstract of his talk.

Also, we have GSOGA funding, so we will have special snacks for tomorrow's coffee hour.

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What: NSAC Coffee Hour talk

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When: Friday, November 3rd, 2017 3:30 pm

Where: BNC Atrium

Speaker: Christopher J Benjamin

Topic: Development of Techniques Using Novel Bioorganic Materials for Protein Structure Determination

​ABSTRACT: Single-particle reconstruction has grown significantly with the improvements in various data collection and computational strategies including CTF fitting, the use of vitrified samples and the utilization of ultra-sensitive direct electron detectors. Although these improvements have contributed significantly to the recent evolution of 3D reconstruction analysis, the way samples are prepared for electron microscopy has remained largely unchanged. We report the development of TEM grids that are modified with non-fouling coatings bearing surface grafted nitrilotriacetic acid substituents that promotes specific capture of protein targets for high-resolution TEM analysis. The utilization of these grids for specific adsorption of the targeted protein onto the grid surface results in well-controlled surface concentration enhancements and a days-to-minutes reduction in time required for the preparation of a purified sample for cryoEM analysis from an E. coli expression system. The selective and reversible capture of his-tag T7 bacteriophage, RplL, and GroEL from crude lysates, as well as purified nanodisc-solubilized his-malFGK2, on these NTA-modified grids with an exceptionally low level of adsorption by non-target proteins, has been observed. Our data illustrate the utility of these grids for selective capture from complex mixtures, detergent-solubilized membrane protein isolates, and expression systems yielding low copy numbers of the desired target in a manner that is well-suited for single particle reconstruction analysis.​

Also, please feel free to sign up for the Spring coffee hour slots in the Google sheet by clicking here. 

Thank you.

Sajid Choudhury

President

Nanotechnology Student Advisory Council (NSAC)

https://engineering.purdue.edu/NSAC/

and 

Ph.D. Candidate,

School of ECE, Purdue University

On Tue, Oct 24, 2017 at 1:04 PM, Sajid Choudhury <sajidmc@purdue.edu> wrote:

We have NSAC Coffee hour this week, Mohamed Hagag from Peroulis group will give a talk on his research. 

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