BME Seminar Distinguished Lecture Series Wednesday, October 10, 2018 9:30-10:20am MJIS 1001 Weldon School of Biomedical Engineering Purdue University? "New Experimental and Data Analysis Methods for Fluorescence Nanoscopy" Keith Lidke, Ph.D. Associate Professor of Physics and Astronomy University of New Mexico [cid:image001.jpg@01D45FC9.6FF501E0] Abstract: Light microscopy, and in particular fluorescence microscopy has the ability to image cellular components - down to single proteins - with high specificity in live or intact cells. The continuing development of contrast agents, e.g., fluorescent tags, and imaging modalities such as super-resolution, light-sheet, and hyperspectral microscopy are pushing the boundaries of achievable spatial-temporal-spectral resolution and multiplexing capabilities. These microscopy techniques produce large imaging data sets that are rich in information and these data sets are often not fully exploited. I will discuss several experimental efforts to improve throughput, multiplexing and spatiotemporal resolution in fluorescence nanoscopy as well as several Bayesian methods to extend resolution and extract kinetic parameters from super-resolution and single particle tracking data. ~BME Faculty Host: Fang Huang~ ***Coffee and juice will be provided at West Lafayette*** -- Fang Huang Assistant Professor Weldon School of Biomedical Engineering College of Engineering, Purdue University West Lafayette, IN 47907 office: 206 S Martin Jischke Dr West Lafayette, IN 47907 Room # 2025 Phone: +1 765 494 6216
