BME Master's Defense Announcement for Jacob Larsen (E. Nauman, advisor) Everyone is invited to attend the public presentation beginning at 11 AM. Title: Characterization of chemical and mechanical properties of porcine brain tissue in vitro Date and Time: Monday, April 10th at 11 AM Location: Zoom (https://purdue-edu.zoom.us/j/94322528882?pwd=cGRxZlMzUXo4ZHpRUm9oc1JlQmxkUT0...) Committee: Dr. Eric A. Nauman, Chair Dr. Vitaliy L. Rayz Dr. Yunjie Tong Abstract: Traumatic brain injury (TBI) is characterized by a violent or sudden blow to the head that causes tearing or bruising of the brain tissue and its supporting blood vessels. Determination of the mechanical properties of gray and white matter is critical for the creation of computational models of healthy and TBI-damaged brain tissues. Current in vivo methods to characterize brain tissue, such as 3D amplified MRI (aMRI) and magnetic resonance elastography (MRE), are highly vulnerable to motion artifacts and have limited techniques to exert mechanical loads on the brain. Therefore, in vitro testing was employed to estimate the chemical composition of gray and white matter using Fourier Transform Infrared (FTIR) spectroscopy and the stress responses of the brain tissues to high compressive deformations via unconfined compression. Attenuated total reflectance (ATR) was run in conjunction with FTIR spectroscopy to eliminate the need for sample preparation. Unconfined compression of gray and white matter samples was performed to 70% of the total sample height at a constant strain rate of 0.5 Hz. Results showed significant increases in the absorbances of white matter (p < 0.05) in the characteristic lipid and carbohydrate regions of the FTIR spectra when compared to gray matter. Within the initial 10% toe-region of the stress-strain curve, white matter is observed to absorb significantly greater compressive loads (p < 0.05) than gray matter. These results indicate an incomplete characterization of brain tissue; therefore, additional in vitro and in vivo methods are still necessary in combination to accurately characterize brain tissue mechanics in TBI and non-TBI patients.