Thanks, Jackie Linnes for sharing this RFP - EMBRIO folks you may be interested: The Chan Zuckerberg Initiative<https://chanzuckerberg.com/> (CZI) has requested applications for collaborative research projects focused on measuring metabolism across organelles and cells. According to CZI, the ability to characterize temporally and spatially the broad molecular profiles, heterogeneity, and phenotypic diversity of organelles and cells and how they interact within and as systems are key to measuring human biology, understanding disease mechanisms, and finding treatments and cures. This request for applications (RFA) aims to accelerate innovative discoveries in metabolism and metabolic physiology in health and disease. Link to complete RFP<https://chanzuckerberg.com/rfa/measuring-metabolism-across-scales/>. Deadline: June 1 The long-term goal of this opportunity is to investigate the metabolic processes that maintain physiological homeostasis at the sub-cellular and cellular levels and understand how and when these normal processes go awry. This two-year funding opportunity is explicitly aimed at addressing the mechanisms of metabolism, including its dynamics and resolution, molecular drivers, and the effects of genetic and environmental risk factors on relevant sub-cellular and cellular properties and interactions, and precisely mapping metabolic changes and states across various scales using a broad range of technologies. The projects should aim to use diverse experimental systems beyond immortalized cell lines to ensure the broadest possible insights into cellular and organelle biology. These grants are not intended to support translational research, clinical trials, or drug development. Examples of potential areas within the scope of this RFA include, but are not limited to: profiling organelles and cells to understand their heterogeneity and metabolic regulation in maintaining homeostasis and how they dynamically evolve to adapt to all types of diverse stressors and metabolic conditions; using metabolic rewiring to study the state and fate of specialized cells (e.g., immune cells, adipocytes, neurons, hepatocytes, etc.) programmed by metabolic processes; using mechanistic studies to understand the function and dysfunction of metabolism at the systems physiology level and provide a framework for understanding the molecular and cellular basis of metabolic diseases; identifying, measuring, and studying molecular level metabolite-protein interactions at scale with high sensitivity and selectivity; developing techniques for metabolic tracing to help identify the origin and fate of metabolites and their contribution to cellular processes and interactions; and establishing metabolomics and/or proteomics methodologies (Mass Spectrometry- and non-Mass Spectrometry-based approaches) to enable high-quality spatial biomolecular analysis of individual organelles and cells. Applications for two types of grants are welcome: expanded and focused projects. The maximum budgets for proposed projects are $500,000 total costs for expanded projects and $250,000 total costs for focused projects. All project awards will be for a 24-month duration. Kim Jacobs Administrative Assistant, Herman B Wells Center for Pediatric Research Department of Pediatrics Indiana University School of Medicine 1044 West Walnut Street Indianapolis, IN 46202 Cell: 317.833.0324 kimjacob@iu.edu [cid:7b235d82-7a7f-46f5-abdc-b2a55e0c21a3] <https://www.purdue.edu/research/embrio/>