Purdue University School of Chemical Engineering Dr. Giorgio Carta "Transport Phenomena in New and Improved Stationary Phases for Downstream Processing of Biopharmaceuticals" November 6, 2012 9:00-10:15 a.m. FRNY G140 Reception at 8:30 a.m. in Henson Atrium Abstract: Downstream processing of pharmaceutical proteins such as monoclonal antibodies (mAbs) is currently limited by capacity and rate bottlenecks. Binding capacity is limited by the large size of these biomolecules while their slow diffusion requires long processing times. The key to removing these bottlenecks is thus to devise new and improved stationary phases for protein chromatography where both capacity and mass transfer rates are increased without sacrificing mechanical strength so that more can be adsorbed faster. Composite materials, incorporating soft, charged polymers supported by a rigid structure have been introduced for this purpose in recent years and appear to be a significant step in the desired direction. But how do these materials achieve faster transport without comprising capacity? The combined use of macroscopic measurements, microscopic measurements using confocal laser scanning microscopy to track the movement of proteins within the chromatographic particles, and transport modeling provides mechanistic insight. We show that protein capacity is increased by multilayer binding while transport is enhanced by close-range biomolecular interactions within the charged polymers giving rise to a large diffusional driving force. We also address the large effects that subtle charge variants arising from post-translational modifications have on chromatography and transport in these materials. Mechanistic models and conditions under which these composite matrices show promise for various applications are illustrated and an outlook for the future is provided. Open Pore Matrix Polymer Grafted [cid:image005.png@01CDB915.9D507540] [cid:image006.png@01CDB915.9D507540] Bio: Giorgio Carta received his PhD in chemical engineering from the University of Delaware in 1984. Since then he has been a faculty member in the Department of Chemical Engineering at the University of Virginia, Charlottesville, Virginia, USA, where he is currently the Lawrence R. Quarles Professor. He currently teaches Mass and Energy Balances and Bioseparations Engineering. His research interests are in bioseparations with emphasis on adsorption, ion exchange and chromatography. Professor Carta has authored more than 125 refereed journal articles in the areas of mass transfer, adsorption, chromatography, and biocatalysis, is the holder of two US patents, a co-author of Section 16 Adsorption & Ion Exchange of Perry's Chemical Engineers' Handbook, 7th and 8th Editions, and co-author of the book "Protein Chromatography - Process Development and Scale-Up" published by Wiley-VCH in 2010. He has served on the Organizing Committee of the International Symposium on Preparative Chromatography - PREP annually since 1997 and as Chair of PREP2009, PRE2011, PREP2012, and PREP2013. He has also served on the Scientific Advisory Committee of the Fifth, Eighth, and Tenth, and Eleventh International Conference on Fundamentals of Adsorption - FOA, as Vice Chair of FOA9, and as Chair of AIChE's Area 2e Adsorption & Ion Exchange and Area 2g Bioseparations. He is currently serving on the editorial boards of the journals Separation Science & Technology and Adsorption, as senior editor of Biotechnology Journal, and as a Member of the Board of the International Adsorption Society. Professor Carta has been involved extensively in continuing education and professional development courses. He has served as lecturer in the ASME Bioprocess Technology Seminars annually from 1991 to 2002, as organizer and leader of the PREP Symposium Workshop on Mass Transfer in Liquid Chromatography annually since 1997, as a lecturer for the Society of Bioprocessing Professionals Annual Bioprocessing Institute from 2003 to 2006, as a lecturer in a biannual short course on Fundamentals of Protein Chromatography at BOKU, Vienna, and the organizer and director of a Short Course in Protein Chromatography held annually at the University of Virginia. He has also served as organizer and lecturer of in-house training programs in process chromatography for pharmaceutical, biotech, and chromatography media companies. Professor Carta is Fellow of AIChE, member of ACS, and Board Member of the International Adsorption Society, and a consultant for chemical, pharmaceutical, and process chromatography companies. Mrs. Karen Heide Forney Hall of Chemical Engineering Purdue University 480 Stadium Mall Drive West Lafayette IN 47907-2100 Phone: 765-494-4050 Fax: 765-494-0805