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Dr.
Paul Hudson
Associate Professor
Department of Protein Science,
School of Engineering Sciences in Chemistry,
Biotechnology and Health,
KTH Royal Institute of Technology
Dr. Paul Hudson is associate professor in the School of Biotechnology at KTH Royal Institute of Technology in Stockholm, Sweden. He
is a graduate of UC Berkeley (2009 Ph.D. ChE) and NC State University (2004, B.S. ChE) and did post-doctoral training at KTH (2012). He is a Fellow of Science for Life Laboratory (2014), a national laboratory focusing on molecular biology, and a Novo Nordisk
Fonden Ascending Investigator (2020). His research is on metabolic engineering of CO2-fixing bacteria, with focus on Calvin cycle bacteria such as cyanobacteria. The research uses systems biology methods such as proteomics to study CO2 fixation metabolism,
and to guide engineering strategies for enhancing CO2 uptake and conversion. His group also develops synthetic biology tools in these strains. He is editor of the 2021 book Cyanobacteria Biotechnology (Wiley Advanced Biotechnology Series).
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Metabolite Regulation of the Bacterial Calvin Cycle
Revealed by Interaction Proteomics
Thursday, October 27, 2022
3:00 p.m. – 4:15 p.m.
Virtual (link below)
Metabolite-level regulation of enzyme activity allows microbes to quickly regulate metabolic pathways during environmental
shifts. This same regulation can complicate metabolic engineering strategies, as pathway flux becomes restricted. However, our knowledge of such regulations is limited for most microbial strains. Here I will describe our work using recently developed proteomics.
methods, such as limited proteolysis small molecule mapping (LiP-SMap) to identify metabolite-protein interactions in the proteomes of cyanobacteria and lithoautotrophic bacteria that fix CO2 using the Calvin cycle. Clustering analysis of the hundreds
of detected interactions showed that some metabolites interacted in a species-specific manner, such as interactions of glucose-6-phosphate in Cupriavidus necator and of glyoxylate in Synechocystis sp PCC6803. Metabolite interactions were tested
for their effects on enzyme activity with Calvin cycle enzymes fructose-1,6/sedoheptulose-1,7-bisphosphatase (F/SBPase). The Calvin cycle intermediate GAP activated both Synechocystis and Cupriavidus F/SBPase, indicating a feed-forward
activation of the Calvin cycle in both photoautotrophs and chemolithoautotrophs. I will discuss implications of these findings for metabolic engineering in these strains.
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