[Kwok, Tim]
d
Dear All,
Below please find the inaugural issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center. The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news).
There are three sections in the newsletter:
3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue
3D in publication – this brings a collection of recent publications on 3D cell culture
3D in meeting – this is a list of upcoming meetings related to 3D cell culture
The newsletter will be available every two months. If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe.
UPCOMING! We will organize a training workshop on the basics of standard cell culture in February 2016 and, most likely in March 2016, a training workshop on the basics of 3D cell culture. Detailed information will be distributed in January.
Please contact me if you have questions.
Yours Sincerely,
Tim Kwok
Facility Manager
3D Cell Culture Core (3D3C) Facility
Birck Nanotechnology Center
Purdue University
Volume 1, December 2015
I
have the privilege to begin the presentations of work from scientists involved in research related to cell culture models for this inaugural newsletter of the 3D Cell Culture Core (3D3C) facility. My interest in 3D cell culture dates back from my graduate
studies in the 90s when I realized by serendipity, as cells modified their arrangement on their own in my culture flasks, how differently cancer cells may behave when they are allowed to form nodules rather than being confined to spread flat over a plastic
surface. Therefore, for my postdoctoral training I joined the laboratory of Mina Bissell, a pioneer for the study of cell-extracellular matrix interaction and the use of 3D cell culture to reproduce tissue architecture, at the Lawrence Berkeley National Laboratory.
There I established different methods for 3D cultures of human non-neoplastic and tumor breast cells and studied the organization of the cell nucleus under these conditions. At Purdue University, for the purpose of improving the study of nuclear functions
in differentiation and cancer, my laboratory has developed methods to better mimic physiologically relevant tissue situations via perfecting cell cultures to reproduce the polarized feature of normal breast epithelia for low and high-throughput production.
Furthermore, in collaboration with engineers Teimour Maleki and Jim Leary at Birck, we designed the first breast-on-a-chip model with branched ductal structures at a time when interest for reproducing normal organs-on-a-chip focused mainly on tissues thought
to be important for drug development (e.g., liver, lung, kidney, intestine). This step allowed us to produce subsequently, in collaboration with the Ziaie group, the first published cancer-on-a-chip (we called it disease-on-a-chip) in which tumors grow surrounded
by their neighboring normal epithelium in curved tissue geometry similar to that of the ducts in which breast cancers arise. This model permitted the demonstration that tissue geometry has a significant influence on nuclear organization and drug sensitivity,
raising the issue of the legitimacy of drug screening models in which tumors are disconnected from major features of their organ context.
At Birck I have discovered a rich multidisciplinary environment to further develop 3D cell culture models both for standard systems using defined extracellular environments
and for more sophisticated organs-on-chip. It is my wish that interactions among engineers and biologists strengthen and multiply for the benefit of science and humanity.
Sophie Lelièvre, DVM, LLMPH, PhD
Professor, Basic Medical Sciences
Scientific Director, 3D Cell Culture Core (3D3C) Facility
3D
in Publications
Recent publications on 3D culture
(please click to access the list on our web page https://nanohub.org/groups/3d3cfacility):
The research papers and reviews are arranged in the following category:
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Scaffold free/Scaffold
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Organ/Tissue/Cell
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Others |
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3D
in Meetings
High-Content Analysis & Phenotypic Screening Congress
11th to 12th February 2016
San Diego, CA, United States of America
Website:
http://www.highcontentanalysis.com/
The High-Content Analysis meeting will focus on the next steps of technology development, including screening of 3D and physiologically relevant complex models, ultra-high resolution and high-throughput imaging, more advanced
image analysis and data management, and new assays and applications.
Organized by: Cambridge Healthtech Institute
Cell Culture World Congress 2016
23rd to 24th February 2016
Sofitel Munich Bayerpost, Bayerstrasse 12, München, 80335, Germany
Website:
http://atnd.it/36181-0
Contact person: Katy Scrivener
This is where pharma, biotechs, researchers & start-ups gather to develop new strategies and partnerships to advance biotherapeutic production, manufacture, and commercialisation.
Organized by: Terrapinn