My apologies, today’s seminar is at
10:00am.
Reminder: BNC Virtual Faculty Seminar Series TODAY @ 10:00am.
ECE and Birck Joint Virtual Seminar
Krishna Jayant
Assistant Professor of Biomedical Engineering
November 12th,
2020 | 10:00am
Join Zoom Meeting:
https://purdue-edu.zoom.us/j/94730880530
Title:
Nanoscale electrical and optical neurotechnologies for mapping the sub-cellular neural code
Abstract: Single neuron computations span multiple scales – from synapse to dendrite to the soma. Mapping single neuron input-output electrical transformations as well as decoding their computational rules
remains a central question in neuroscience and a key need for a thorough understanding of both microcircuit function as well as behavioral output. In this talk I will present both published and ongoing work in which we exploit multimodal approaches to address
key challenges in the field of single neuron computation. In the first part of my talk, I will describe our work on mapping dendritic spine electrical dynamics. Dendritic spines, characterized by a small head (volume~0.01-0.1ìm3) and narrow neck (diameter~0.1ìm,
length~1ìm), are the primary site of excitatory synaptic input in the mammalian brain. Synaptic inputs made onto spines first integrate onto dendrites, and subsequently propagate towards the soma and axon initial segment, where they further integrate with
other inputs to determine overall action potential output. Elucidating the electrical properties of spines is thus paramount for understanding the first steps along this signal processing chain. I will describe our work using quantum-dot labeled quartz nanopipettes
(15-30 nm diameters) under two-photon visualization for targeted intracellular recordings from spines1 and small pre-synaptic terminals. I will show through detailed experiments that (i) spines receive large EPSPs (25-30mV), and (ii) estimated neck resistances
are large enough to influence electrical isolation (mean ~420 M?), and filter synaptic input as it invades the dendrite. I will then briefly describe the theoretical implications of these properties2 and describe key advances in amplifier design that can enable
previously unattainable experiments on spines4. I will then describe recent results from my lab using two-photon holography to stimulate spines in 3D to unravel dendritic spine co-operativity across dendrites. In the second part of this talk, I will
switch gears and describe a new method in which I combine the flexible property of these nanopipettes with microprisms to enable simultaneous two-photon calcium imaging and targeted intracellular electrophysiology from dendrites in vivo during behavior3. Last,
I will give a succinct overview of our recent work at Purdue, including two-photon compatible transparent nanoelectrode arrays for mapping dendritic activity from the surface of the awake-behaving brain. I will conclude by presenting a snapshot of key collaborative
projects at Purdue that highlight the integration of nanoscience and neuroscience.
1 Jayant, K. et al. Targeted intracellular voltage recordings from dendritic spines using quantum-dot-coated nanopipettes. Nature Nano, (2017).
2 Thibault Lagache, Krishna Jayant & Rafael Yuste,. Electrodiffusion modelling of spine voltage dynamics. J. Comp. Neurosci, 2019
3 Jayant, K. et al. Flexible nanopipettes for motion-insensitive intracellular electrophysiology in vivo. Cell Reports (2019).
4. Shekar, S., Jayant, K., et al, A miniaturized multi-clamp CMOS amplifier for intracellular neural recording, Nature Electronics, 2019
Bio: Krishna Jayant received his B.Tech degree in electrical engineering from the National Institute of Technology (NIT) Trichy, India in 2005, where as part of his bachelor’s thesis he worked on bio-inspired
optimization techniques. After brief research stints at IISc Bangalore (2005-2006), and the University of Bologna (2006-2007), both in the area of microelectronics, he joined Cornell University, Ithaca NY, where he received his M.S/PhD in electrical engineering
in 2014 working with Prof. Edwin C. Kan on Bioelectronics. He then joined Columbia University for postdoctoral research training with Profs. Rafael Yuste, Ken Shepard, and Ozgur Sahin in the field of circuit neuroscience, biophysics, and CMOS integrated neurotechnology.
Here, he was awarded the prestigious Kavli Post-Doctoral Fellowship for carrying out work at the intersection of nanoscience and neuroscience. As an Assistant Professor of Biomedical Engineering at Purdue, his laboratory focuses on elucidating the biophysical
features involved in neural computation and their behavioral relevance using a combination of nano-electrophysiology, optical microscopy, CMOS integrated systems, and modeling. His research is supported by the NIH Trailblazer Award, the HFSP young investigator
grant, and the Ralph E. Powe Junior Faculty Enhancement Award.
Upcoming BNC Virtual Faculty Seminars, Fall 2020:
|
Date |
Faculty |
Title |
|
9/10/20 |
Ali Shakouri, Director of Birck Nanotechnology Center |
Overview of SMART Industry Consortium |
|
9/17/20 |
Luna Lu, Professor of Civil Engineering - *Note time change* (10am - 11am): Co-Hosted with ECE |
Nanotechnology for Energy Harvesting and Sensing Applications |
|
9/24/20 |
Alex Ma, Assistant Professor of Physics and Astronomy |
Superconducting circuits for quantum simulation and quantum information sciences |
|
10/01/20 |
Hadiseh Alaeian, Assistant Professor of Electrical and Computer Engineering |
atom-Nanophotonics at low dimensions |
|
10/08/20 |
Jianguo Mei, Assistant Professor, Department of Chemistry
|
Semiconducting Polymers: A Journey from Scientific Curiosity to Technology Commercialization |
|
10/15/20 |
Chen-Lung Hung, Assistant Professor of Physics and Astronomy
|
Engineering atom-nanophotonics hybrid lattices |
|
10/22/20 |
Chi Hwan Lee, Assistant Professor of Biomedical Engineering and Mechanical Engineering |
Sticker-Like Flexible Sensors for Wearable Healthcare Applications |
|
10/29/20 |
Ryan Wagner, Research Assistant Professor, School of Mechanical Engineering |
Atomic force microscopy as a tool for multiphysics nanoscale characterization |
|
11/5/20 |
Arnab Banerjee, Assistant Professor of Physics and Astronomy |
Co-design towards exotic spin-based systems and devices |
|
11/12/20 |
Krishna Jayant, Professor of BME - *Note time change* (10am - 11am): Co-Hosted with ECE |
|
|
11/19/20 |
Xiulin Ruan, Professor, School of Mechanical Engineering |
First Principles and Machine Leaning Predictions of Thermal and Optical Properties, and the Developments of Radiative Cooling Paints |
Spring 2021 Schedule:
|
Date |
Faculty |
Title |
|
2/4/21 |
Yi Xie, Assistant Professor, School of Nuclear Engineering |
Degradation of Structural Materials and Fuels in Nuclear Systems |
|
2/11/21 |
Letian Dou, Assistant Professor, Davidson School of Chemical Engineering |
Organic-Perovskite Hybrid Quantum Wells, Heterostructure, and Optoelectronics |
|
2/18/21 |
Nikhilesh Chawla, Ransburg Professor, School of Materials Engineering |
4D Materials Science – Probing Microstructural Evolution Through Time-Resolved Imaging |
|
2/25/21 |
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|
3/4/21 |
David Cappelleri, Associate Professor of Mechanical Engineering |
Microrobotics Research Highlights |
|
3/11/21 |
Stylianos Chatzidakis, Assistant Professor, School of Nuclear Engineering |
|
|
3/18/21 |
Reading Day |
|
|
3/25/21 |
Tyler Tallman, Assistant Professor, School of Aeronautics and Astronautics |
|
|
4/1/21 |
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4/8/21 |
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4/15/21 |
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4/22/21 |
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4/29/21 |
Dead Week |
|
|
5/6/21 |
Finals Week |
Thanks!
Jaime Turner
Lead Administrative Assistant to the Director | Birck Nanotechnology Center
BRK | 1205 W State Street | West Lafayette, IN 47907
o: 765-494-3509 | m: 765-491-3064 | jjturner@purdue.edu
