Dear Fellow Scientists, The 3D Cell Culture Core (3D3C) facility is proposing a first hands-on training workshop for basic 3D cell culture techniques on June 16 and 17, 2016. The purpose of 3D cell culture is to place cells in an environment in which they reproduce physiologically relevant features of real tissues. There is a wide range of techniques involved in the setup of the 3D cell culture. The workshop in June will cover some basic techniques for work with tumors or certain other tissues that require the formation of masses or spheroids; advanced and specialized techniques of 3D culture for other types of tissue architectures and coculture techniques will be covered via additional workshops in the future. For details on the content, prerequisite and registration regarding the “Basics of 3D Cell Culture” see below and the flyer attached. What is referred to ‘spheroid’ culture using gels is relatively simple to set up and is used in many studies for cancer research. The June workshop will cover the setup of spheroid culture using drip and thick embedding methods with EHS (engelbreth-holm-swarm)-derived gel, better known as Matrigel type of gel, as it is the most widely used gel at the moment, but the workshop will cover the use of gels (natural, like collagen I better suited for the study of invasive tumors for instance, and artificial) in general. It will also cover using serum-free medium, dislodging multicellular structures from hydrogel-based 3D culture and preparing 3D cultures for immunolabeling. The prerequisite for the workshop is sufficient knowledge in standard (2D) cell culture techniques and, of course, REM biosafety training and blood-borne-pathogen training. If you are interested in the workshop, please contact me for information and registration. The workshop is limited to SIX participants. The deadline for registration is May 20, 2016. In closing, each model can be tailored to the need of a particular study. Therefore, there is more than techniques involved; there is a way of thinking. At 3D3C we hope to trigger interest in this process and help you optimize ongoing and future research projects that make use of cell culture. Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/5432b8a7-5264...]