March 2017 - BNC-all - ecn.purdue.edu

3D3C Newsletter - February 2017
by Kwok, Tim 13 Mar '17

13 Mar '17

Dear All, Below please find the eighth issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center. The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news) The four sections in the newsletter are: 3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue 3D in focus – this section presents the current work on a specific 3D cell culture model or technique 3D in publications – this section brings a collection of recent publications on 3D cell culture 3D in meetings – this section includes a list of upcoming meetings related to 3D cell culture The newsletter will be available every two months. If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe. Please contact me if you have questions. Yours Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/3e8e050f-67f…] Volume 8, February 2017 [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/10f15e72-d4d…] 3D at Purdue 3D in Focus 3D in Publications 3D in Meetings 3D at Purdue The Savran Laboratory focuses on the development of novel platforms for rare cell detection and analysis. Especially in the context of the disease of cancer, isolation of rare cells from blood samples of patients is extremely important as it offers a glimpse into the tumor using a simple blood test. One important downstream application for such methods is testing of drugs in a personalized manner. One can isolate the tumor cells of a particular patient and find out which drugs are effective in killing these cells before trying drugs directly on the patient. However tumor cells are found in blood samples only in extremely small amounts. This is a challenge because one normally needs a lot of cells to test sufficient combinations and dosages of drugs. It is therefore important to culture the isolated cells and increase their number. While culturing the cells, it is important to mimic conditions that the cells experience in their natural environment as much as possible so that the resulting tests can correlate with realistic outcomes. This can best be achieved with 3D culture conditions where cells are suspended in a manner that surrounds them with tissue-like milieu, as opposed to 2D methods where cells attach to a flat surface, a condition that is not natural. We are collaborating with Drs. Lelièvre and Kwok to develop novel methods to culture single rare cells in a manner that allows high throughout analysis and testing. We expect that these studies will enhance our understanding of heterogeneity and pave the way to personalized therapy. Professor Çağrı A. Savran School of Mechanical Engineering School of Biomedical Engineering (by courtesy) School of Electrical and Computer Engineering (by courtesy) ________________________________ 3D in Focus Osteoarthritis is one of the leading causes of chronic disability for elderlies. One of the possible causes of the disease is synovial inflammation by macrophage activation. The objective of the article highlighted in this newsletter was to build and validate a 3D coculture system for osteoarthritis inflammation through paracrine interactions between chondrocytes and macrophages. Featured article: Satyavrata Samavedi, Patricia Diaz-Rodriguez, Joshua D. Erndt-Marino, and Mariah S. Hahn. A Three-Dimensional Chondrocyte-Macrophage Coculture System to Probe Inflammation in Experimental Osteoarthritis Tissue. Tissue Engineering: Part A 2017, 23: 101-114. In the transwell system, normal chondrocytes (NCs) cultured in the upper chamber and activated macrophages (AMs) cultured in the bottom chamber were both placed in a 3D environment by encapsulation within poly(ethylene glycol) diacrylate hydrogels. Increased levels of matrix metalloproteinases (MMPs) and proinflammatory cytokines that are associated with early stages of osteoarthritis were observed in NCs cells in the coculture system. Such increases were also detected in osteoarthritic chondrocytes (OACs) in coculture with AMs, although the levels of MMPs and proinflammatory cytokines were already enhanced in OACs due to the natural disease process. The OACs cultured in the presence of nonactivated macrophages demonstrated a lower increase in levels of MMPs and proinflammatory cytokines, indicating that AMs may intensify the abnormal matrix degradation and cytokine secretion in OACs. In addition, AMs cocultured with OACs expressed significantly more IL-1b and VEGF-A compared to AMs alone, suggesting that OACs may also intensify abnormal activation in macrophages. Advantages of the proposed model: Despite the primary location of the macrophages in the knee joint within the synovial membrane, in the previously reported models of in vitro osteoarthritis, only the chondrocytes were cultured in a 3D microenvironment; the macrophages were cultured in 2D as a monolayer. The significance of the present model is to culture both chondrocytes and macrophages in a 3D environment. Moreover, the authors of the present report investigated the phenotypic changes in macrophages in coculture with chondrocytes; these cells have not been a focus of study in previous work dealing with osteoarthritis. Furthermore, the use of diseased rather than normal chondrocytes may enable the modeling of the later stages of osteoarthritis progression; this seems an appropriate model for testing potential therapeutic approaches of the disease. Next possible steps: Osteoarthritis progression involves many steps and interactions among different cell types and extracellular matrices. The work in the highlighted article, however, examined only the interaction between chondrocytes and macrophages for synovial inflammation. Moreover, models that recapitulate various stages in osteoarthritis are still needed. This work shows like other models before that is possible to obtain interesting answers even when using cell types from two different species (human chondrocytes vs. mouse macrophages). Future studies using primary human macrophages in the coculture system will be important to confirm the interaction between chondrocytes and macrophages described here. ________________________________ 3D in Publications Recent publications on 3D culture (please click to access the list on our web page https://nanohub.org/groups/3d3cfacility) Review<https://nanohub.org/groups/3d3cfacility/news#reviews> The research articles and reviews are arranged in the following categories: Scaffold free/Scaffold Organ/Tissue/Cell Others Spheroids<https://nanohub.org/groups/3d3cfacility/news#spheroids> Organoid<https://nanohub.org/groups/3d3cfacility/news#organoid> Scaffold<https://nanohub.org/groups/3d3cfacility/news#scaffold> Hydrogel<https://nanohub.org/groups/3d3cfacility/news#hydrogel> Matrix<https://nanohub.org/groups/3d3cfacility/news#matrix> Microfluidics<https://nanohub.org/groups/3d3cfacility/news#microfluidic> Microfabrication<https://nanohub.org/groups/3d3cfacility/news#microfabrication> Bone<https://nanohub.org/groups/3d3cfacility/news#bone> Bone Marrow<https://nanohub.org/groups/3d3cfacility/news#bonemarrow> Breast<https://nanohub.org/groups/3d3cfacility/news#breast> Colon<https://nanohub.org/groups/3d3cfacility/news#colon> Heart<https://nanohub.org/groups/3d3cfacility/news#heart> Liver<https://nanohub.org/groups/3d3cfacility/news#liver> Lung<https://nanohub.org/groups/3d3cfacility/news#lung> Muscle<https://nanohub.org/groups/3d3cfacility/news#muscle> Nerve<https://nanohub.org/groups/3d3cfacility/news#nerve> Prostate<https://nanohub.org/groups/3d3cfacility/news#prostate> Endothelial cells<https://nanohub.org/groups/3d3cfacility/news#endothelialcells> Fibroblast<https://nanohub.org/groups/3d3cfacility/news#fibroblast> Stem Cells<https://nanohub.org/groups/3d3cfacility/news#stemcells> Stromal Cells<https://nanohub.org/groups/3d3cfacility/news#stromalcells> Cancer/Tumor<https://nanohub.org/groups/3d3cfacility/news#cancer> Screening<https://nanohub.org/groups/3d3cfacility/news#screening> 3D bioprinting<https://nanohub.org/groups/3d3cfacility/news#3dbioprinting> Imaging<https://nanohub.org/groups/3d3cfacility/news#imaging> ________________________________ 3D in Meetings Keystone Symposia - Engineered Cells and Tissues as Platforms for Discovery and Therapy Date: 9th to12th March 2017 Location: Boston, Massachusetts, United States Website: https://www.keystonesymposia.org/17K1 Contact: Phone: 1 800-253-0685; Email: info(a)keystonesymposia.org<mailto:info@keystonesymposia.org> Organized by: Keystone Symposium Cell Culture World Congress USA 2017 Date: 23rd to 24th May 2017 Location: San Diego, California, United States Website: http://go.evvnt.com/68105-0 Contact person: Freya Smale This event allows research scientists engaged in upstream and downstream bioprocessing to discuss novel techniques and techniques and technologies. Organized by: Terrapinn USA The 3rd Annual 3D Cellular Models: Engineering Predictive Preclinical Screening Models Date: 14th to 15th June 2017 Location: Boston, Massachusetts, United States Website: http://www.worldpreclinicalcongress.com/3D-Cellular-Models/ Organized by Cambridge Healthtech Institute The 2nd International Conference "Plant Cells In Vitro: Fundamentals and Applications" Date: 26th to 27th June 2017 Location: Vienna, Austria Website: http://viscea.org/index.php/plant-in-vitro Contact person: Alisher Touraev The Conference will discuss wide range of modern in vitro plant cell and organ culture technologies, fundamental aspects of plant cell totipotency, differentiation, regeneration, embryogeneisis, and practical applications of in vitro technologies. Organized by: Vienna International Science Conferences & Events Association (VISCEA)

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BNC E-news for the week of March 13th
by Turner, Jaime J 13 Mar '17

13 Mar '17

Seminars Seminar: Atom Probe Tomography and its Applications Matt Pietrucha and Dan Lawrence CAMECA (Madison, WI) March 22, 2017 @ 3:30pm in BRWN 1154 School of Nuclear Engineering, Purdue ALL-USER MEETING Did you miss the All-User Meeting? You have until Friday March 31st to go through the slideshow with your professor and hand in the signed paper work to BRK 1220. http://www.purdue.edu/discoverypark/birck/operations/safety.php has the slideshow and paperwork you need to fill out. NANODAYS Volunteers Needed!!! Volunteers can attend any or all of the following shifts: Wednesday: 4/5/17, 5:30 PM - 8:00 PM Thursday: 4/6/17, 8:45 AM - 11:30 AM 4/6/17, 11:15 AM - 2:00 PM Friday: 4/7/17, 8:45 AM - 11:30 AM 4/7/17, 11:15 AM - 2:00 PM Training is mandatory and will be provided in the Birck atrium. The schedule is: Tuesday: 3/28/17, 5:00 PM - 6:00 PM (Pizza Party) 6:00 PM - 7:30 PM (Training Sessions) Interested volunteers can sign up here. (http://tinyurl.com/ztb83wz) A full list of activities can be found here. (http://tinyurl.com/hzbkkna) Questions can be directed to volunteer coordinators Joon Park (park218(a)purdue.edu<mailto:park218@purdue.edu>), Justin Wirth (jcwirth(a)purdue.edu<mailto:jcwirth@purdue.edu>), and Sean Rinehart (srinehar(a)purdue.edu<mailto:srinehar@purdue.edu>). User Information Solvent Hood Use Please remember that only "Intrinsically safe" hotplate are allowed in solvent hoods. With the recent fire in a hood this is a major safety concern. Also housekeeping is also an issue. Too much "stuff" making it nearly impossible was also a contributing factor to the situation. Any standard hotplate found In the hoods will be removed fi they are not "intrinsically safe/ explosion proof. Stephen Jurss Safety Office

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Circle Drive in front of MRGN
by Totten, Mary J 09 Mar '17

09 Mar '17

Duhme Drive parking lot closed week of March 13 The Duhme Drive horseshoe parking lot east of the North Martin Jischke Drive and West State Street intersection will be closed for use beginning March 13 while its parking is reconfigured. Work is expected to be completed by the end of the week. Upon reopening, both sections of the parking lot will temporarily allow for two-way traffic with access available from First Street only. The entrances off West State Street will be closed in preparation for the State Street Redevelopment Project. Later this year, the parking lot will be permanently readjusted back to one-way traffic patterns, and access from both First Street and West State Street will be available upon completion. Motorists should seek alternate parking locations while work is performed and obey all posted signs. Questions may be directed to Don Petersen, managing director, at (765) 496-8335 or drpeter(a)purdue.edu.

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Vibration Alert - Demolition of Housing North of the BNC
by Voorhis, Mark L. 08 Mar '17

08 Mar '17

Dear BNC User: I was notified today that the demolition of buildings in front (north of) the BNC will begin next Monday (3/13/17). This is part of the Innovation and South Street project. The demolition is expected to continue for approximately 3-4 weeks. The contractor will be working from 7:00 am until 5:30 pm Mon - Fri. It is possible that the BNC may experience vibration during this demolition process. Please be aware of this possibility and organize your work accordingly. Also, please notify me at mvoorhis(a)purdue.edu<mailto:mvoorhis@purdue.edu> if you experience any vibration issues in your research as a result of this demolition. Thank you in advance for your understanding during this Purdue University Physical Facilities (PUPF) project. From: Denecke, Jacob M Sent: Wednesday, March 08, 2017 1:37 PM To: Voorhis, Mark L. Subject: PVIL Demolition | Next Week Look Ahead Mark, Per our discussion, here is a look ahead for next week: We expect that heavy demo will commence on or after 3-13-17 at the far East end in front of Birck and will progress East to West toward Airport road. The buildings are masonry and concrete floor plates on bar joists and will come done easily. In the process of bringing them down it is possible that large sections of masonry wall or the floor plates will impact with the ground causing vibrations. The work hours will be 7 am to 5:30 pm M-F and the possibility of Saturday work as well. Within each building is a slab on grade and also a basement which will have to be demolished and could also cause some vibrations. The basement areas and utility trenches will have to be backfilled in lifts and vibratory compaction equipment will be used and will cause vibrations. We are currently erecting silt fence and other erosion control measures and installing tree protection fencing and other site prep, asbestos abatement and utility disconnections from the buildings. I'll send you additional updates as they become available. Please let me know if you would like additional person(s) CC'd on these updates. Please direct all PVIL demolition communication through me. As always, if you have any questions, please do not hesitate to contact me. Best Regards, Jacob Denecke, P.E. Capital Project Manager Purdue University Capital Program Management Freehafer Hall of Administrative Services 401 S. Grant Street West Lafayette, IN 47907-2024 Ph: 765.494.6721 | Fax: 765.494.0918 | Cell: 815.762.2397

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Cell Line Database - 2nd Announcement
by Kwok, Tim 07 Mar '17

07 Mar '17

Dear Colleague, As mentioned in the email dated Feb 13, 2017, the 3D3C (3D Cell Culture Core) facility is setting up a database of cell lines available in the research laboratories of Purdue University with information on their use in 3D culture if applicable and on culture protocols. At the moment, information on more than 100 cell lines has been included in the database. We would like to thank those who contribute to the database already. For those who would also like to contribute, you can always input the cell line information through the following link, https://purdue.qualtrics.com/SE/?SID=SV_bkeXZ5Xef10qP3L or email the following information to me (kwokt(a)purdue.edu) Name of cell line Tissue of origin of the cell line Use with serum-free medium: Yes or No Use in 3D culture as well: Yes or No In the future, if you would like to know whether a specific cell line is available in the Purdue community, please feel free to contact me. I will search the database to identify the cell line(s) available, then contact the individual faculty who has the cell line(s) of interest, and upon her/his approval send the contact information to the requester. Hopefully, the database will help foster new collaborations among scientists at Purdue. Thank you for your participation. Yours sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University

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Dr. David Warsinger - Nanomanufacturing Preeminent Team Faculty Seminar - TODAY @ 9:30am in BRK 1001
by Turner, Jaime J 06 Mar '17

06 Mar '17

[cid:image001.png@01D29355.CE9B8E90] Jaime Turner Administrative Assistant to the Director Birck Nanotechnology Center 1205 West State Street West Lafayette, IN 47907-2057 Email: jjturner(a)purdue.edu<mailto:jjturner@purdue.edu> Phone: 765-494-3509

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New program to improve fabrication quality at Birck - input requested by March 24
by Peroulis, Dimitrios 03 Mar '17

03 Mar '17

Dear all, In an effort to improve fabrication quality at Birck, we are starting a new program focused on process characterization, monitoring, and control. Essentially, we would like our engineering staff to help you achieve consistent and repeatable results by regularly running and characterizing your critical fabrication processes in the cleanroom. This could have multiple benefits: 1. Improved quality control and repeatability 2. Provides early warning for process and equipment problems 3. Effectively creating and maintaining process database and lab memory 4. Active engagement of engineering staff into research 5. Lowering fabrication cost and time for everyone This effort will be successful only with active participation of faculty and their research group. As a first step, we would like to invite each group to document the steps for a couple of critical processes they follow on a regular basis. For example, it could be a lithography process (document PR, recipes, measurement method), or an oxide deposition process (document sample preparation, deposition recipe, measurement method). In the beginning students/postdocs will be training engineering staff to run these processes. In the long run the staff members will run these on a regular basis and maintain a database. This will be a dynamic program with new processes being added and obsolete ones being retired on a regular basis. Priority will be given to processes that benefit multiple groups. Please send these processes to Ron and I by March 24, 2017 in a ppt format. After this, we will have a meeting to group these and discuss a plan to get started. We would very much welcome any thoughts and ideas you have on how to make this successful and improve everyone's fabrication results at Birck. Best, Dimitri ________________________________________________________________________________ Dimitrios Peroulis Professor, School of Electrical and Computer Engineering Deputy Director, Birck Nanotechnology Center Purdue University 1205 West State Street West Lafayette, IN 47907-2057 tel: (765) 494-3491 email: dperouli(a)purdue.edu<mailto:dperouli@purdue.edu> http://sites.google.com/site/peroulisteam/ ________________________________________________________________________________

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Today's NSAC Coffee Hour
by Aveek Dutta 03 Mar '17

03 Mar '17

Dear all, Please join us for today's NSAC coffee hour session in the Birck atrium at 3:30 pm. Details follow: Presenter: Punyashloka Debashis Title: Computation with Nanomagnets Regards, Aveek Dutta Graduate Research Assistant Birck Nanotechnology Center Department of Electrical and Computer Engineering Purdue University

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BNC E-news for the week of March 6th
by Turner, Jaime J 02 Mar '17

02 Mar '17

Seminars Dr. David Warsinger Seminar Monday, March 6th @ 9:30am in BRK 1001 Title: Can we save lives with thermodynamics? Nanoengineering and Thermofluids for the Water-Energy-Food Nexus Link to abstract and bio: http://www.purdue.edu/discoverypark/birck/events/index.php?view=2260 Dr. Haimei Zheng Seminar Tuesday, March 7th @ 1:30pm in BRK 1001 Title: Materials Transformations and Dynamic Phenomena at Solid-Liquid Interfaces Link to abstract and bio: http://www.purdue.edu/discoverypark/birck/events/index.php?view=2261 Dr. Huan Hu Seminar Thursday, March 9th @ 9:30am in BRK 1001 Title: Advancing Nanomanufacturing for Crucial Healthcare Applications Link to abstract and bio: http://www.purdue.edu/discoverypark/birck/events/index.php?view=2262 Nanodevices for Bio-inspired Computing Julie Grollier Centre National de la Recherche Scientifique (CNRS) Thales Research and Technology Laboratory, Palaiseau, France. Tuesday, March 7th from 2:00 - 3:15 PM MRGN 121 It is once again our pleasure to extend a warm invitation to the 34th Herbert C. Brown Lectures in Organic Chemistry at Purdue University. Date: Friday, April 14, 2017 Time: 9:00 AM - 6:00 PM (EST) Location: Stewart Center (STEW) 218 This year's event promises to be an exciting day celebrating and honoring the career of Professor H.C. Brown. The program includes a diverse array of topics from the fields of synthetic and physical Organic Chemistry by a distinguished group of plenary speakers: Jeffrey Bode, ETH Zürich: "Protein Synthesis with the KAHA Ligation" Samuel Gellman, University of Wisconsin - Madison: "Impact of Backbone Modifications on the Information Content of Peptidic Foldamers" Kazunori Kataoka, The University of Tokyo: "Fantastic Voyage by Supramolecular Nanosystems: Challenge from Polymer Chemistry Toward Smart Targeted Therapy of Intractable Diseases" Scott J. Miller, Yale University: "Searching for Selective Catalytic Reactions in Complex Molecular Environments" All interested students, faculty, and scientists are welcome to attend. Registration is free, and includes lunch for all participants who register by Friday, April 7, 2017. We also encourage student participants to present their recent works at our poster session and competition, with prizes awarded for the best posters. For more information about the H.C. Brown Lectures, please visit our website at: https://www.chem.purdue.edu/hcbrownlectures/. We look forward to seeing you on April 14, 2017. ALL-USER MEETING Did you miss the All-User Meeting? You have until Friday March 31st to go through the slideshow with your professor and hand in the signed paper work to BRK 1220. http://www.purdue.edu/discoverypark/birck/operations/safety.php has the slideshow and paperwork you need to fill out. NANODAYS Volunteers Needed!!! Volunteers can attend any or all of the following shifts: Wednesday: 4/5/17, 5:30 PM - 8:00 PM Thursday: 4/6/17, 8:45 AM - 11:30 AM 4/6/17, 11:15 AM - 2:00 PM Friday: 4/7/17, 8:45 AM - 11:30 AM 4/7/17, 11:15 AM - 2:00 PM Training is mandatory and will be provided in the Birck atrium. The schedule is: Tuesday: 3/28/17, 5:00 PM - 6:00 PM (Pizza Party) 6:00 PM - 7:30 PM (Training Sessions) Interested volunteers can sign up here. (http://tinyurl.com/ztb83wz) A full list of activities can be found here. (http://tinyurl.com/hzbkkna) Questions can be directed to volunteer coordinators Joon Park (park218(a)purdue.edu<mailto:park218@purdue.edu>), Justin Wirth (jcwirth(a)purdue.edu<mailto:jcwirth@purdue.edu>), and Sean Rinehart (srinehar(a)purdue.edu<mailto:srinehar@purdue.edu>). User Information Dear Users of the FE SEM Hitachi S-4800 at BRK 1235, Unfortunately, approximately twice a week or even more, our SEM is down due one particulate repetitive mistake: assembling the sample holder. We are using a new base of a sample holder and therefore, please familiarize yourself how the SEM sample holder should be assembled. The simple cartoon is attached and the print out is on the wall of the SEM prep lab, BRK 1233. Please ask the SEM support staff if you have any question. The wrong assembly results in dropping the sample holder in the SEM chamber. It takes about 4 hours to make the SEM operational again and the other several hours to operate the SEM normally at high voltages. This compromises the research of the other user. Please aware that now your account might be billed for the staff time to recover the system and the lost SEM time. However, as a bonus for the wrong assembling the sample holder, we will place your "design" on the Wall of Shame in BRK 1233. Discovery Park Chief Scientist's Blog [cid:image005.jpg@01D29379.88DB8760]<https://www.purdue.edu/discoverypark/blog/> Discovery Park is pleased to announce the launch of the new Discovery Park Chief Scientist's Blog<https://www.purdue.edu/discoverypark/blog/>. In the blog, our Chief Scientist and Executive Director, Tomás Díaz de la Rubia<https://www.purdue.edu/discoverypark/blog/about-the-chief-scientist/>, will share his accounts of our initiatives and the impact our teams are having in and across our three strategic areas: Global Health, Global Sustainability and Global Security. We invite you to follow along for updates on all that's happening at Discovery Park and more information on how you can get involved.

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