March 2016 - BNC-all - ecn.purdue.edu

Birck Business Manager
by Anthrop, Heather L 10 Mar '16

10 Mar '16

I am happy to announce that Erica Cox has accepted the Birck Business Manager position. She will transition to the position effective April 1st.<x-apple-data-detectors://0> Erica comes to us from the ECE business office and prior to that she was in the English department business office. Please join me in congratulating Erica on joining our team! Donna Brown

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Dr. Dmitry Zemlyanov Colloqium - Thursday, March 10th @ 3pm in ARMS 1109
by Turner, Jaime J 09 Mar '16

09 Mar '16

[cid:image001.png@01D179F3.494C8360] Jaime Turner Birck Nanotechnology Center 1205 West State Street West Lafayette, IN 47907-2057 Email: jjturner(a)purdue.edu<mailto:jjturner@purdue.edu> Phone: 765-494-3509

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BNC E-news for the week of March 7th
by Anthrop, Heather L 04 Mar '16

04 Mar '16

100X Objective Outside the Cleanroom BIRCK CLOSURE E-Beam Lithography REFRIGERATOR Room Changes Seminar Travel iTap Shutdown 100X Objective Outside the Cleanroom A user asked if an optical microscope with 100X lens was available outside the cleanroom, which would be convenient for doing work on samples without gowning up. We have an Olympus microscope, now in the new J-Bay characterization lab, but it only goes up to 50X. We're getting quotes for a 100X objective and will compare the cost to the income generated by the scope on recharge to see if this purchase would be feasible. Thanks for the suggestion. BIRCK CLOSURE NOTICE: The BNC will be completely closed for one day on March 17, 2016 from 12:01AM to 11PM. Only people involved in the shutdown will be allowed in the building during the shutdown. All offices, laboratories, and the cleanroom will be closed. All chemicals and research inside fume hoods will need to be placed in closed containers prior to March 17, 2016 from 12:01AM This is an advance notice on March 17, 2016 from 6AM to 9PM Purdue Physical Facilities Department will complete an emergency power interruption to fix a high voltage power issue with our center's fire pump controller. Per safety codes, power to the fire pump transfer/control was hardwired directly from the electrical substation to the fire panel when the building was constructed. This wiring bypasses our main switchboard and provides no lockout point for the fire panel. Therefore, for safety reasons the electric substation providing power to the fire pump has to be shutdown. The affected substation also supplies power to one of the building's three, 3000 amp switchboards. Only essential personnel will have access to the building during the power outage since the building will be without fire protection during that time. All CLEANROOM and LABORATORY research work will be suspended and building access closed during power outage. All exhausted hoods, vacuum systems, and/or toxic gas systems will be shut down, and chemicals inside fume hoods will need to be placed in closed containers prior to March 17, 2016 from 12:01AM. General areas that will be affected by the power outage are listed below. - All chemical exhaust systems for the labs and cleanroom. - Building ambient temperature and humidity control o Offices o General laboratories o Cleanroom o Atrium - Office power and lighting (all computers will lose power) - Atrium power and lighting - Card reader system Systems NOT affected by the power outage are listed below. - Emergency power (on emergency generator power) - UPS power (on emergency generator power) - Equipment emergency power (on emergency generator power) - Cleanroom research equipment power (power from a different switchboard) - Laboratory research equipment power (power from a different switchboard) Please let me know if you have any questions or concerns. Mark Voorhis Building Manager Purdue University Birck Nanotechnology Center Office: (765) 494-3036 E-Beam Lithography A user suggested we purchase an additional 100KV EBL system for use in the cleanroom. We're actively pursuing options with EBL suppliers to understand our options and costs associated with a new and better system. In the meantime, the VB6 has enjoyed several months of good uptime, and we anticipate this will continue as several issues have been found and fixed over many months of service work and system improvements. REFRIGERATOR Everything from the refrigerator and freezer in the kitchen will be trashed at 8 AM March 18. Since the power is off we have no way to determine if the food stayed cold so it will have to be trashed. Take it home before the 17th if you do not want it trashed. Questions or concerns please see Nancy Black. Room Changes In order to accommodate Nano Days several meetings had to be moved to different locations. All the times are the same but please note the new room and building. Prof. Garimella Group Meeting - April 13 moved from BRK 1001 to MRGN 129 MicroICE Meeting - April 13 - moved from BRK 1099 to BRK 2290 Birck Orientation - April 14 - moved from BRK 1099 to BRK 2290 INDEX - April 14 - moved from BRK 1099 to BRK 1024 Prof. Qi Group Meeting - April 14 - moved from BRK 1201 to BRK 1290 Prof. Shakouri Group Meeting - April 14 - moved from BRK 1201 to BRK 2024 Prof. Shalaev Group Meeting - April 15 - moved from BRK 2001 to MANN 203 Yuchen Du Preliminary Exam - April 15 - moved from BRK 2001 to MRGN 206 Prof. Peroulis Group Meeting - April 15 - moved from BRK 2001 to MRGN 206 iLab Implementation Team - April 15 - moved from BRK 1001 to MRGN 206 Prof. Savran Group Meeting - April 15 - moved from BRK 1001 to DLRC 143A SMART Film Meeting - April 15 - moved from BRK 1001 to DLRC 143A Seminar Shunsuke Murai Seminar Monday, March 7th at 10:00 am in Birck 2001 Plasmonic Array to Tailor the Emission For more information please visit: https://nanohub.org/groups/bnc/calendar/details/1441 Travel As a reminder you must submit a trip request into Concur before traveling on official Purdue business. If you do not know how to do this please ask. iTap Shutdown WORK TO BE PERFORMED: From 12:00 AM to 1:00 AM on Friday, 3/11/16, the ITaP Data Networks Team will be performing scheduled work that will impact the campus Firewall System. AFFECTED CLIENTS: The following campus departments and services will be impacted by this maintenance: 1. AGIT 2. BBC (Bindley Bio Center) 3. BIO (Biology) 4. COP (College of Pharmacy) 5. ECN 6. General NAT Service for 10.X address space 7. IDAP 8. ITCR Labs and TIC sites 9. KRAN (School of Management) 10. ITIS NetMgmt 11. USDA 12. IT Video (STEW Broadcast studios) 13. VoIP 14. Wireless SCOPE OF WORK: Affected Client(s) should plan for a 1-minute network outage to occur at some point during the scheduled outage window for services running through the campus firewall. If you have any questions or concerns about this work please don't hesitate to let us know. Thanks much. Duane =============================== Duane Kyburz, Network Engineer Purdue University - ITaP, IT Infrastructure Services Data Networks Team Office: 765-496-8226 Mobile: 765-490-4755 Fax: 765-494-6620 Email: storm(a)purdue.edu<mailto:storm@purdue.edu>

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NSAC Coffee hour
by Anthrop, Heather L 04 Mar '16

04 Mar '16

Please join NSAC today at 3:30 pm for coffee hour featuring Jiawei Zhou speaking about An Ultrasonically Controlled Power Management System (UCPMS) for Implantable Biomedical Devices.

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Reminder: Spring 2016 Social Event: Bowling at PMU Friday March 4 from 5pm to 7pm
by NSAC 03 Mar '16

03 Mar '16

Dear All, This is a gentle reminder that you are invited to a social event hosted by NSAC, OSA and SPIE Purdue Chapter! There will be food, bowling, and lots of fun. So please come to relax for a while. Where: Purdue Memorial Union (PMU) Rack n Roll bowling When: Tomorrow (Friday), March 4th, from 5PM to 7PM *Please let us know if you would like to attend by filling the form at the link: *http://bit.do/Bowling_at_PMU See you there! NSAC, OSA and SPIE Officers On Tue, Mar 1, 2016 at 12:58 PM, NSAC <nsac(a)purdue.edu> wrote: > Dear All, > > You are invited to a social event hosted by NSAC, OSA and SPIE Purdue > Chapter! There will be food, bowling, and lots of fun. So please come to > relax for a while. > > Where: Purdue Memorial Union (PMU) Rack n Roll bowling > When: Friday, March 4th, from 5PM to 7PM > > > *To confirm that you'll be attending, simply indicate so at the link: * > http://bit.do/Bowling_at_PMU > > See you there! > > > > NSAC, OSA and SPIE Officers > >

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3D3C Newsletter - Feb 2016
by Kwok, Tim 02 Mar '16

02 Mar '16

Dear All, Below please find the second issue of the newsletter of the 3D Cell Culture Core (3D3C) Facility of the Birck Nanotechnology Center. The newsletter is also available online (https://nanohub.org/groups/3d3cfacility/news) There are four sections in the newsletter: 3D at Purdue – this section highlights 3D cell culture-based research activity at Purdue 3D in discussion – this section presents the current work on a specific 3D cell culture model or techique 3D in publication – this section brings a collection of recent publications on 3D cell culture 3D in meeting – this section includes a list of upcoming meetings related to 3D cell culture The newsletter will be available every two months. If you do not wish to receive the 3D3C newsletter in the future, please reply “cancel” to unsubscribe. Please contact me if you have questions. Yours Sincerely, Tim Kwok Facility Manager 3D Cell Culture Core (3D3C) Facility Birck Nanotechnology Center Purdue University ________________________________ [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/3e8e050f-67f…] Volume 2, February 2016 [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/10f15e72-d4d…] 3D at Purdue 3D in Discussion 3D in Publications 3D in Meetings 3D at Purdue In the Ziaie Biomedical Microdevices Laboratory (ZBML), we combine the design concepts of MEMS/BioMEMS technology with modern rapid prototyping equipment to develop novel devices and microsystems that address important clinical problems. We use equipment such as laser engravers, cutter-plotters, and 3D printers to process and repurpose a multitude of materials which are exotic to traditional MEMS fabrication but invaluable for medical applications. Such materials include ultra-soft elastomers (for conforming to tissue/organs), a broad range of functional polymers with unique surface properties (for automatic response to changing microenvironments), as well as paper and fabrics (for creating inexpensive, wearable, and disposable diagnostics/therapeutics). 3D printing is particularly advantageous for creating structures with complex morphologies which mimic the intricate micro-architectures found in nature (e.g., micro-scales or micro flanges in insects and plants). One recent application of 3D design in our lab is a practical fabrication technique for creating polymeric microneedles of complex geometries for drug/vaccine delivery applications, Figure 1 [1]. This technique allows the creating of more sophisticated, slanted, or out-of-plane designs that allow for easier needle insertion using smaller shear force. We achieve this by coupling 3D printing technology with an isotropic shrinkage technique, which effectively enhances the current resolution limits of 3D printing by at least five fold. The resulting needles are sufficiently sharp to penetrate porcine skin and deliver loaded/embedded chemicals. [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/33a1d70a-3f9…] Figure 1: Photographs of polymeric microneedles. (a) and (c) show two 3D printed molds of different sizes; (b) and (d) show shrunk replicas of each mold after two gel-shrinking iterations. Tip radius of curvature (boxed in yellow) is (a) r = 212 µm; (b) r = 14.7 µm; (c) r = 56 µm; (d) r = 9.6 µm. (e) Final dissolvable polymeric needles. (f) Alternate complex 3D mold. We are also investigating the use of 3D architectures for cancer research in collaboration with Prof. Lelièvre. By combining the diverse but complementary expertise of both labs, we are engineering device platforms for culturing cancer cells in a 3D environment which closely mimics the human physiology in order to create improved tumor models for evaluating anti-cancer drugs. One example of this is the development of laser-machined hemi-microchannels as models of mammalian breast cancer cells, Figure 2 [2]. By culturing cells along the curved walls of a hemichannels, their physiology is able to more accurately mimic tumor development within the epithelial environment of mammary ducts, providing a framework for the design and test of anticancer therapies. [https://gallery.mailchimp.com/b7d6d1ffee56a866e499104cf/images/2d851ff5-a00…] Figure 2: Monolayer of cells on a smooth semicircular acrylic channel. SEM images (left) show the appearance of each type of hemichannel before seeding the cells. Nuclei are stained with DAPI (blue). Scale bars, 10 μm. We look forward to accelerating the pace of high-impact medical research by using such rapid prototyping fabrication technologies along with fruitful inter-disciplinary collaborations at Purdue. Babak Ziaie, PhD Professor, Electrical and Computer Engineering [1] M. Ochoa, J. Zhou, R. Rahimi, V. Badwaik, D. Thompson, and B. Ziaie, “Rapid 3D-print-and-shrink fabrication of biodegradable microneedles with complex geometries,” in Proc. Transducers - 2015 (Anchorage, AK), 2015. [2] P.-A. Vidi, T. Maleki, M. Ochoa, L. Wang, S. M. Clark, J. F. Leary, and S. A. Lelièvre, “Disease-on-a-Chip: Mimicry of Tumor Growth in Mammary Ducts,” Lab Chip, 2013. 3D in Discussion Research Paper: Wang JD, Khafagy ES, Khanafer K, Takayama S, ElSayed ME. Organization of Endothelial Cells, Pericytes, and Astrocytes into a 3D Microfluidic in Vitro Model of the Blood-Brain Barrier. Mol Pharm. Epub ahead of print DOI: 10.1021/acs.molpharmaceut.5b00805 The blood–brain barrier (BBB) consists of endothelial cells lining the brain capillaries system and surrounding astrocytic glial membranes. The barrier function is dependent on the tight junctions between the endothelial cells and the specific transport proteins in the cell systems. The BBB lets some substances, such as water, oxygen, carbon dioxide, and general anesthetics, pass into the brain. It also keeps out bacteria and other substances, such as many anticancer drugs. The BBB is the bottleneck in brain drug development and is the single most important factor limiting the growth of neurotherapeutics. The BBB can be measured by in vivo, in vitro, and in silico system (Bickel U, J Am Soc Exp NeuroTherapeutics, 2005, 15 - 26). An effective in vitro model for BBB will allow their use for noninvasive, rapid, economic, and reproducible screening of the BBB permeability of new drug candidates. However, the current in vitro BBB models fail to replicate the organization and restrictive behavior observed in vivo due to poor integration of the endothelial cells with supporting cells (pericytes and astrocytes) following the correct anatomical organization observed in vivo. The manuscript described an in vitro BBB model by co-culture of mouse brain microvascular endothelial cells, pericytes, with astrocytes in layered microﬂuidic channels forming three-dimensional models of the BBB. The model exhibits diﬀerent degrees of “restrictiveness” indicated by their high transendothelial electrical resistance (TEER) values, low permeability of [14C]-mannitol and [14C]-urea, and functional expression of the P-glycoprotein efflux pump. Based on the similarity in mannitol permeability across cell monolayers in the BBB model with the permeability reported for the BBB in vivo and the high functional expression of the P-glycoprotein eﬄux pump, the tri-culture BBB model described in this report represents a 3D in vitro model of the BBB that closely mimics its restrictive nature observed in vivo. The work described in the manuscript is a typical case for 3D tissue model development, the crossover of cell biology and engineering technology. 3D in Publications Recent publications on 3D culture (please click to access the list on our web page https://nanohub.org/groups/3d3cfacility) Review<https://nanohub.org/groups/3d3cfacility/news#reviews> The research papers and reviews are arranged in the following category: Scaffold free/Scaffold Organ/Tissue/Cell Others Spheroids<https://nanohub.org/groups/3d3cfacility/news#spheroids> Organoid<https://nanohub.org/groups/3d3cfacility/news#organoid> Scaffold<https://nanohub.org/groups/3d3cfacility/news#scaffold> Hydrogel<https://nanohub.org/groups/3d3cfacility/news#hydrogel> Matrix<https://nanohub.org/groups/3d3cfacility/news#matrix> Microfluidics<https://nanohub.org/groups/3d3cfacility/news#microfluidic> Microfabrication<https://nanohub.org/groups/3d3cfacility/news#microfabrication> Adipocyte<https://nanohub.org/groups/3d3cfacility/news#adipocyte> Bladder<https://nanohub.org/groups/3d3cfacility/news#bladder> Bone<https://nanohub.org/groups/3d3cfacility/news#bone> Bone Marrow<https://nanohub.org/groups/3d3cfacility/news#bonemarrow> Breast<https://nanohub.org/groups/3d3cfacility/news#breast> Colon<https://nanohub.org/groups/3d3cfacility/news#colon> Heart<https://nanohub.org/groups/3d3cfacility/news#heart> Liver<https://nanohub.org/groups/3d3cfacility/news#liver> Lung<https://nanohub.org/groups/3d3cfacility/news#lung> Muscle<https://nanohub.org/groups/3d3cfacility/news#muscle> Nerve<https://nanohub.org/groups/3d3cfacility/news#nerve> Prostate<https://nanohub.org/groups/3d3cfacility/news#prostate> Endothelial cells<https://nanohub.org/groups/3d3cfacility/news#endothelialcells> Fibroblast<https://nanohub.org/groups/3d3cfacility/news#fibroblast> Stem Cells<https://nanohub.org/groups/3d3cfacility/news#stemcells> Stromal Cells<https://nanohub.org/groups/3d3cfacility/news#stromalcells> Plant Cells<https://nanohub.org/groups/3d3cfacility/news#plantcells> Cancer/Tumor<https://nanohub.org/groups/3d3cfacility/news#cancer> Screening<https://nanohub.org/groups/3d3cfacility/news#screening> 3D bioprinting<https://nanohub.org/groups/3d3cfacility/news#3dbiprinting> Imaging<https://nanohub.org/groups/3d3cfacility/news#imagining> Quantitation<https://nanohub.org/groups/3d3cfacility/news#quantitation> 3D in Meetings EMBO|EMBL Symposium: Tumour Microenvironment and Signalling Conference 3rd to 6th April 2016 EMBL Advanced Training Centre, EMBL Meyerhofstrabe 1, Heidelberg 69117, Germany Website: http://atnd.it/39532-0 Contact person: Course and Conference Office This symposium brings together researchers from complementing fields to enhance our understanding of the communication between cancer cells and their microenvironment. Organized by: European Molecular Biology Laboratory, EMBL Heidelberg World Stem Cells and Regenerative Medicine Congress 2016 Conference 18th to 20th May 2016 London, United Kingdom Website: http://atnd.it/36213-0 Contact person: Katy Scrivener Europe’s market place for investment, commercial opportunities and collaboration. Focusing on commercialising cell therapies, stem cells for drug discovery, tissue engineering and organ regeneration. Time: £50 - £3070. Organized by: Terrapinn 2016 International Forum on Medical Physics, Biomedical Engineering and Biotechnology Conference 14th to 15th July 2016 Osaka, Japan Website: http://www.astfi.org/IFMBB2016/index.html Contact person: Christopher Zhu The conference aims to foster and conduct collaborative interdisciplinary research in state-of-the-art methodologies and technologies within Medical Physics, Biomedical Engineering and Biotechnology. Organized by: Asia Science and Technology Forum Institute EMBO|EMBL Symposium: Organoids: Modelling Organ Development and Disease Conference 12th to 15th October 2016 Heidelberg, Germany Website: http://atnd.it/39583-0 Contact person: Course and Conference Office This conference will bring together the leading researchers in organoid field to establish a new research community and reveal parallels between various tissue models. Organized by: European Molecular Biology Laboratory, EMBL Heidelberg

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Spring 2016 Social Event: Bowling at PMU Friday March 4 from 5pm to 7pm
by NSAC 01 Mar '16

01 Mar '16

Dear All, You are invited to a social event hosted by NSAC, OSA and SPIE Purdue Chapter! There will be food, bowling, and lots of fun. So please come to relax for a while. Where: Purdue Memorial Union (PMU) Rack n Roll bowling When: Friday, March 4th, from 5PM to 7PM *To confirm that you'll be attending, simply indicate so at the link: * http://bit.do/Bowling_at_PMU See you there! NSAC, OSA and SPIE Officers

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White Jetta 340 LOP lights are on
by Black, Nancy Lee 01 Mar '16

01 Mar '16

It has been reported to me that a White Jetta 340 LOP lights are on in our parking lot. Thank you, Nancy Black Secretary V Birck Nanotechnology Center West Lafayette, IN 47907-2057 Phone: 765-494-8675 Fax: 765-494-4731

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