BME Master's Defense Announcement for Umm E Hani Abdullah (T. Kinzer-Ursem, advisor) Everyone is invited to attend the public presentation beginning at 11:00AM EST. Title: Deep brain stimulation in the Syngap1+/- mouse model of autism and intellectual disability Date: November 28, 2022 Time: 11:00AM EST Location: MJIS 2001 and Zoom (meeting info below*) Thesis Committee: Dr. Tamara Kinzer-Ursem (Major Professor) Dr. Maria Dadarlat Makin Dr. Karin Ejendal Abstract: Syngap1+/- haploinsufficiency is phenotypically characterized by autism spectrum disorder (ASD), intellectual disability (ID), and epilepsy. SynGAP as a protein, is also known as Synaptic Ras GTPase-activating protein, regulating the function in synapses. Treatment-resistant severe ASD in particular, presents concurrently with ID in 31% patients, where patients exhibit minimal speech formation, self-injurious behavior, and cognitive impairment. Previously, success in alleviating self-injurious behavior along with speech formation was demonstrated in adolescent patients undergoing deep brain stimulation (DBS) of the basolateral amygdala (BLA). In addition, SynGAP protein is an integral component of the post-synaptic density and its role in signaling pathway converges with other autism risk genes and consequently autism risk proteins. A critical gap exists in understanding how electrical stimulation as part of the Hebbian theory, can induce neuroplasticity. Therefore, we sought to develop a chronic DBS model in Syngap1+/- haploinsufficient mice that can be used to assess the behavior and proteomic correlates due to DBS in BLA. We optimized the DBS surgery and anesthesia requirements to ensure survival of Syngap1+/- mice as they are more susceptible to isoflurane anesthesia. Additionally, we optimized the DBS stimulation parameters in accordance with behavior responses from Syngap1+/- mice by performing multiple trials of DBS paremeters. These methods ensure attainment of local field potentials from Syngap1+/- mice - future directions include verifying the role of DBS in producing theta oscillations for the purposes of memory retrieval. *Hani Abdullah is inviting you to a scheduled Zoom meeting. Topic: Hani Abdullah's Master's Defense Time: Nov 28, 2022 10:00 AM America/Indiana/Indianapolis Join Zoom Meeting https://purdue-edu.zoom.us/j/98616521806?pwd=clUrVkhVa1plTE5kSDduZlJIREZaQT0... Meeting ID: 986 1652 1806 Passcode: 185740 One tap mobile +13092053325,,98616521806#,,,,*185740# US +13126266799,,98616521806#,,,,*185740# US (Chicago) Dial by your location +1 309 205 3325 US +1 312 626 6799 US (Chicago) +1 646 558 8656 US (New York) +1 646 931 3860 US +1 301 715 8592 US (Washington DC) +1 305 224 1968 US +1 253 205 0468 US +1 253 215 8782 US (Tacoma) +1 346 248 7799 US (Houston) +1 360 209 5623 US +1 386 347 5053 US +1 507 473 4847 US +1 564 217 2000 US +1 669 444 9171 US +1 669 900 6833 US (San Jose) +1 689 278 1000 US +1 719 359 4580 US Meeting ID: 986 1652 1806 Passcode: 185740